Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt

... Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis Laura Caldinelli, Gianluca Molla, Mirella S. Pilone and Loredano ... structure, retains flavin binding, and shows an increased sensitivity to trypsin of a specific site (Arg289) belonging to the loop preceding the b-strand F5 of the...

Ngày tải lên: 30/03/2014, 11:20

... cross-wise and diploids were selected on plates lacking leu- cine and tryptophan. Diploid cells were resuspended and dropped in equal amounts on to plates lacking histidine and adenine with increasing ... data in yeast suggest that the Alr1 protein acts as a Mg 2+ -permeable ion channel [14]. Using in vitro chemical cross-linking and in vivo split-ubiquitin assays to analyz...

Ngày tải lên: 19/02/2014, 06:20

... mutations in this interface can in uence DNA binding as well as effector binding, albeit both ligand binding sites are not in direct contact to these altered residues. This finding represents a ... of DNA binding and 4-ddma- atc dependent corepression in TetR i2 . V99T, on the other hand, shows partial DNA binding, corepression by atc and induction by 4-ddma-atc when introdu...

Ngày tải lên: 20/02/2014, 01:20

... sites in proteins with xenon or krypton. Proteins 30, 61–73. 52. Rubin,S.M.,Lee,S Y.,Ruiz,E.J.,Pines,A.&Wemmer,D. (2002) D etection and characterization of xenon -binding s ites in proteins ... structures o f proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2. Pro...

Ngày tải lên: 07/03/2014, 16:20

... structures. At 10 min, punctate staining diminished and vacuolar staining in- creased, and finally punctate staining was almost lost with predominant vacuolar staining at 30 min. Com- pared with ... for lipid binding and a putative coiled-coil motif for pro- tein–protein interaction. In this study, Osh6p was demonstrated to bind a pool of phosphatidylinositol phosphates (PtdInsP) i...

Ngày tải lên: 07/03/2014, 21:20

... used in this evaluation were trained with observation data from the Lancaster Treebank training set. The training set and testing set are sub-divided from the corpus in proportions of 2 3 and 1 3 . ... micro-average F -measure against the number of training sentences during text chunking (A: MHHMM, B: HHMM and C: HMM) The first finding is that the size of training data dramaticall...

Ngày tải lên: 08/03/2014, 02:21

... interactions between FadD29 and PKS15 ⁄ 1 on the one hand, and FadD26 and the FasI system on the other hand. Further inves- tigations, including protein interaction studies and structural studies, ... with [1- 14 C]propionate, a precursor known to be incorpo- rated into methyl-branched fatty acyl-containing lipids, including DIMs. Lipids were then extracted from the recombinant stra...

Ngày tải lên: 15/03/2014, 11:20

... their actin -binding domains. To date, none of the actin -binding domain sequences registered in databases is present in hhLIM or CRPs. The goals of this study were to define the actin -binding properties of ... domain plays a central role in bundling of F-actin. Taken together, these data identify hhLIM as an actin -binding protein that increases actin cytoskeleton stability by pr...

Ngày tải lên: 16/03/2014, 06:20

... of domains in DMPK and homologous kinases, such as the myotonic dystro- phy kinase-related Cdc42 -binding kinase (MRCKa ⁄ -b- c) [1,2], ROCK-I ⁄ -II [3] and Citron kinase [4], placed DMPK in the ... clear. Activation of Citron kinase and ROCK-I is mediated by RhoA binding to a Rho -binding domain located in the C-terminal part of the coiled-coil region 1[4,8,19]. In this res...

Ngày tải lên: 16/03/2014, 13:20

... ndogenous peroxidase activity, cells were treatedwithNaCl/P i containing 3% h ydrogen peroxide and 10% methanol for 15 min; nonspecific binding with PKCa and PKCe was blocked by incubation for 30 min with ... roles in the regulation of APP processing and sAPPa release in SH-SY5Y cells – the former being involved predominantly i n the response to direct activation of the kinase and...

Ngày tải lên: 23/03/2014, 13:20