Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
... Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme Urszula Kosinska 1 *, ... posi- tion in the substrate complex from that in the complex with the feedback inhibitor, having moved in closer to the 5¢-OH of the substrate to form a hydrogen bon...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc
... locates on the long loop region followed by Nb4, and the side chain of Val200 faces toward the inside of the c atalytic cleft [39]. Figure 4 shows the h ypothetical structure of the active site of S. ... not shown). These results indicate that the two recombinant a-glucosidases a re identical to their parent enzymes. Substrate specificities of chimeric enzymes Th...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khóa học: Interaction between the 2¢)5¢ oligoadenylate synthetase-like protein p59 OASL and the transcriptional repressor methyl CpG-binding protein 1 potx
... [12]. The products of these three genes are known, respectively, as the small (p42/p46), the medium (p69/p71) and the large (p100) forms of OAS [13], all of which are enzymatically active. The ... prey library from human leukocyte cDNA fused to the trans- activating domain of GAL4. The yeast strain L40 was used for screening the library, enabling selection of b...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... 3. The catalytic efficiencies of 2 for both enzymes were the highest among the synthetic substrates. The value of V max /K m of 2 for the E. freundii enzyme was 1.7-times higher than that for ... °C. The enzyme solution was lyophilized and then used for synthesis of oligosaccharides without further purification. The crude enzyme preparation catalysed the...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx
... [18] and an inacti- vated peptide deformylase [19]. The last of these is very labile under oxygen stress, resulting in the oxidation of the catalytic Fe 2+ center of the deformylase into the inactive ... regions 1–21 and 1–31, the former originating by incomplete cleavage of a Glu-Val bond (Fig. 2). No evidence for cross-linking of the heme with other peptides o...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Schizosaccharomyces pombe positive cofactor 4 stimulates basal transcription from TATA-containing and TATA-less promoters through Mediator and transcription factor IIA ppt
... coding strand of the )35 to +6 region of Ad-MLP. (B) EMSAs used 32 P-labelled sin- gle-stranded DNA from the coding strand of the )35 to +6 region of Ad-MLP as the probe. The double- (dsDNA) and single-stranded (ssDNA) ... the stimulation of promoter escape as well as initiation in the presence of an activator. Therefore, we investigated the effect of PC4 on...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
... reason for the loss of one thioether bond from the mitochondrial cytochromes c of euglenozoans is a longstanding puzzle. It could be a means of altering the structure of these cytochromes c and ... details of the heme attach- ment. Notably, this similarity includes the stereochemistry of the covalent heme attachment to the protein. The structure has impli...
Ngày tải lên: 23/03/2014, 04:21
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc 1 complex may occur due to the binding of ISP and ... 1909 to the 500 kDa bc 1 sub -complex. Therefore, the pres- ence of both Qcr9p and Bcs1p is required for the inser- tion of ISP into the bc 1 sub -comple...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... molecule, the position of the guanine base and most of the phosphate groups of the first nucleotide (Gp) corresponded well with the mononucleotides in the crystal structures. The same was true for the ... and the base of the third nucleotide are located in the vicinity of Thr61 and Arg67–Thr69. In the second cluster, the ribose and the base of...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx
... steps per conformer was performed during the cyana structure calculations. In the final cycle of the cyana protocol, 100 conformers were generated and further refined using the amber 9 software package ... Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (JST), by a Grant-in-Aid for Scientific Research from the Japan...
Ngày tải lên: 18/02/2014, 14:20