Báo cáo khoa học: Lipopeptide structure determines TLR2 dependent cell activation level potx

... 261–277. TLR2 recognition by lipopeptides U. Buwitt-Beckmann et al. 6364 FEBS Journal 272 (2005) 6354–6364 ª 2005 The Authors Journal compilation ª 2005 FEBS Lipopeptide structure determines TLR2 dependent ... the huTLR2 or muTLR2 were selected as a cellular test system. These cells express both TLR1 and TLR6. At first it should be noted that all bioactive LP used in our study were...

Ngày tải lên: 30/03/2014, 11:20

... crystal structures of transketolase, pyruvate oxidase and pyruvate decarboxylase. Structure 1, 95–103. Ó FEBS 2003 3D structure of indolepyruvate decarboxylase (Eur. J. Biochem. 270) 2321 Crystal structure ... The space group and cell dimensions were determined using the auto-indexing option of MOSFLM and by the analysis of pseudo-precession images [18]. Structure determination The...

Ngày tải lên: 20/02/2014, 11:20

... crystal structure of TaLcc1 does not have a chloride attached to the T2 copper, as observed in the crystal structure of MaL. The unique fea- ture of TaLcc1 and MaL as compared with other laccases structures ... three- dimensional structure of TaLcc1 shows both similari- ties to and differences from the analogous structures of the ascomycete laccase (asco-laccase) MaL, thus giv- ing a co...

Ngày tải lên: 14/02/2014, 18:20

... complex (observed structure factors F pep o ) with an apo structure of importin-a (70–529) (observed structure factors F apo o ). This structure is described in ref. [42]. The structure factors ... Inhibitor Cocktail; Roche Applied Science, Penzberg, Germany). The cells were lysed using a French pressure cell and the resulting cell debris was centrifuged at 39 000 g for 45 min a...

Ngày tải lên: 14/02/2014, 19:20

... analysis of structure and thermostability. J Mol Biol 286, 189– 205. 19 Cooper JB, McIntyre K, Badasso MO, Wood SP, Zhang Y, Garbe TR & Young D (1995) X-ray structure analysis of the iron -dependent ... macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 54, 905–921. 39 Murshudov GN, Vagin AA & Dodson EJ (1997) Refinement of macromolecular structures by t...

Ngày tải lên: 14/02/2014, 22:20

... for 6 h at 37 °C, and the cells were washed with new minimal medium (NMM) and finally resuspended in 5 mL NMM. The sec- ond culture was inoculated with 1 mL of washed cells in 100 mL NMM containing ... expression was induced by adding 1 mm isopropyl thio-b-d-galactoside. Cells were harvested 8.5 h after induction. After lysis of the cells the affinity purifica- tion with Ni-NTA was performed. Cr...

Ngày tải lên: 16/02/2014, 09:20

... common tertiary structure [4,13,14]. They fold into an a + b structure with a central five-stranded antiparallel b-sheet and an Keywords cytotoxic protein; NMR; ribonucleases; RNase T1; structure; ... cycles of NOE assignment and structure calculation by cyana ⁄ candid, a set of 20 structures that satisfy the experimental constraints was obtained. The Solution structure of hirsutellin...

Ngày tải lên: 18/02/2014, 08:20

... of the extracellular matrix, it seems more likely that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface ... vein endothelial cells (HUVEC P145) were purchased from Clo- netics (CC-2519). All subsequent cell manipulations were carried out according to the manufacturer’s instructions. Cells were a...

Ngày tải lên: 19/02/2014, 00:20

... between structure and function of a ⁄ b proteins. Q Rev Biophys 13, 317–338. 19 Whittington DA, Rusche KM, Shin H, Fierke CA & Christianson DW (2003) Crystal structure of LpxC, a zinc -dependent ... Taylor A & Lipscomb WN (1990) Molecular structure of leucine aminopeptidase at 2.7-A ˚ resolution. Proc Natl Acad Sci USA 87, 6878–6882. 21 Gouet P, Courcelle E, Stuart DI & Met...

Ngày tải lên: 19/02/2014, 00:20

... ifferent cell types u sed i n t he experiments. The binding sites on the surface of these cells may be unique. MOLT-3 and Jurkat cells are derived from lymphocytes, while sheep blood cells are ... Bierer, B.E. & Burakoff, S.J. (1988) T cells adhesion molecules. FASEB J. 2, 2584–2590. 2. Wang, J.H. & Reinherz, E. (2000) Structure basis of cell cell interactions in the i mmune syst...

Ngày tải lên: 19/02/2014, 13:20