Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by ﬂow linear dichroism technique doc

... 5. Inhibition of topoizomerase II activity measured by the FLD technique. Comparison of the kinetics of inhibition of reaction of 8 lg of pUC19 DNA with 4 lg of topoisomerase II by etoposideand ... Catalytic transformations of supercoiled DNA as studied by ﬂow linear dichroism technique Alexander Gabibov1,2,3,*, Elena Yakubovskaya1, ... continuous assay to measure the kinetics of the scDNAtopoisomerization was performed. The development of such a technique was hindered because of multiple DNA species of intermediate topologypresent...

Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf

... obtained for SGAP and the catalytic mutant [36]. By contrast, the E131D replacement resul-ted in a decrease of four orders of magnitude in kcat,corresponding to an increase of 9 kJÆmol)1in the ... during catalysis was assessed by measuring the isotope effect at different pHs, forthe native enzyme and its catalytic mutant E131D. Theexact mechanistic role of Glu131 was explored by analyzing ... metalloenzymes [53–58]. Inhibition of SGAP by ﬂuoride and phosphate anions was investi-gated by determining the initial rates of the hydrolysis of Leu-pNA as a function of the inhibitor concentra-tion...

Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot

... conformation of ETNF-6mAbSequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc-ture. The heavy chain of ... fragment of  17 kDa. This fragment was digested to a smaller molecule of 15 kDa by scission of the Gln21-Ala22 bond. The intermediate product was furtherconverted into a fragment of 13.3 kDa by successive ... natural catalytic antibodies. The ﬁrst natural catalytic anti-body isolated from the serum of an asthma patientwas reported by Paul et al. [1]. The catalytic antibodycould enzymatically cleave vasoactive...

Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx

... presence of 0.5 mMNADPH. Site-directed mutagenesis of the catalytic tetrad of AKR1B1,composed of Tyr, Lys, His and Asp, revealed that the triad of Asp43,Lys77 and His110, but not Tyr48, acts as ... activity of AKR1B1increased with increasing pH, with an optimum of pH 8.5. The pKavalue of PGDS activity was calculated by Eqn (1) to be 7.94 ± 0.07. Nonenzymatic autode-gradation of PGH2was ... B(2001) Exploring the active site of yeast xylose reductase by site-directed mutagenesis of sequence motifs charac-teristic of two dehydrogenase ⁄ reductase family types.FEBS Lett 500, 149–152.29...

Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

... mgÆmL)1) of 0.97was used. For the isolated wild-type protein (without fusionpartner), A280 of 0.65 was used whereas, for the isolatedTrp mutants, A280 of 0.54 was used.Activation of glucokinase ... elements of the proteins were evaluated by the CD Neural Network algorithm [32]. The global confor-mational stability, as measured by thermal denaturation(5–90 °C), was determined by measuring ... wild-type hGK () by increasing concentrations of Glc. The solid lines represent the ﬁt of the data to two hyperbola as obtained by nonlinear regression analy-ses, giving Kdvalues of 4.8 ± 0.1...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... luciferase reporterassay of the IFN-b gene promoter using the various cells treated with M-pIC. The dual luciferase reporter assay was performed as describedin (A) except that poly(I-C) was added ... luciferase reporter assay were performed as described inFig. 1B. The relative level of the IFN-b gene promoter activity wascalculated as described in (A).Limited suppression of the IFN system by ... In contrast, the caspase recruitmentdomains (CARDs) of MDA5 or RIG-I recruit theCARD adaptor inducing IFN-b, Cardif (also known as IPS-1, MAVS, or VISA), which was recently identi-ﬁed as an...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... suggestive of a catalytic water molecule whichhas been replaced by the acetate moiety. The proteincoordinates zinc with the Ndatom of His12, one of theOdatoms of Asp15 and the Neatom of His113.His113 ... presence of a functional(i.e. zinc-containing) active site is associated with theextent of the backbone distortion of this residue, sothat a certain catalytic role of this Asp appears likely.Based ... temperature factors of theFig. 2. Assembly of the BcZBP hexamer. (A, B) Side and (C) top views of the BcZBP hexamer formed through the association of threedimers. Each dimer has a different color....

Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... promoter, was con-ﬁrmed by plasmid DNA NdeI digestion. Parental plasmidpHC1, lacking the G6PD-6PGL fragment was used as atransfection control. Plasmid DNAs were puriﬁed (PlasmidMaxi Kit, Qiagen, ... determine parasitaemia, about 10 000 erythrocytes were examined and the number of infected erythrocytes was reported as percentage of the total. Stage-speciﬁc development was assessed by counting ... parasitaemia) weretransfected by electroporation with 100–150 lg of puriﬁedplasmid DNA or 40 lg of dsRNA as described [19]. Theparasites transfected with pHC1 or pHC1G6 PD -AS weresubsequently cultured...

Tài liệu Báo cáo khoa học: "The Impact of Query Reﬁnement in the Web People Search Task" docx

... datasets forthis clustering task, with over 15 research groupssubmitting results in each campaign. Since the re-lease of the ﬁrst datasets, this task is becoming anincreasingly popular research ... of reﬁnements are the most successful. In our ex-periments we have considered the search resultsassociated to one individual as a set of relevantdocuments, and we have tested the ability of ... dataset consists of, at most, 100 web pages written in English andretrieved as the top search results of a web searchengine, using the (quoted) person name as query2.Annotators were asked...

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... alkylation of the DNA ladder by mitoDC-81 (Fig. 3B, lane 1).Analysis of the gel prior to electrotransfer showed that theextent of alkylation was proportional to the amount of DNA present and was independent ... was independent of the size of the DNA fragment (Fig. 3A, lane 1). As mtDNA in vivo is negatively supercoiled it was also important to compare the ability of mitoDC-81 to alkylate linear, relaxed-circular, ... forms, supercoiled, linear andrelaxed-circular plasmid DNA, in order of increasingapparent molecular weight (Fig. 3A). Comparison of theethidium ﬂuorescence (Fig. 3A) and the amount of DNA alkylation...

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