Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by flow linear dichroism technique doc

... 5. Inhibition of topoizomerase II activity measured by the FLD technique. Comparison of the kinetics of inhibition of reaction of 8 lg of pUC19 DNA with 4 lg of topoisomerase II by etoposide and ... Catalytic transformations of supercoiled DNA as studied by flow linear dichroism technique Alexander Gabibov 1,2,3, *, Elena Yakubovskaya 1 , ... cont...

Ngày tải lên: 30/03/2014, 11:20

... obtained for SGAP and the catalytic mutant [36]. By contrast, the E131D replacement resul- ted in a decrease of four orders of magnitude in k cat , corresponding to an increase of 9 kJÆmol )1 in the ... during catalysis was assessed by measuring the isotope effect at different pHs, for the native enzyme and its catalytic mutant E131D. The exact mechanistic role of Glu131 was e...

Ngày tải lên: 19/02/2014, 02:20

... conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc- ture. The heavy chain of ... fragment of  17 kDa. This fragment was digested to a smaller molecule of 15 kDa by scission of the Gln21-Ala22 bond. The intermediate product was further converted i...

Ngày tải lên: 06/03/2014, 22:21

... presence of 0.5 m M NADPH. Site-directed mutagenesis of the catalytic tetrad of AKR1B1, composed of Tyr, Lys, His and Asp, revealed that the triad of Asp43, Lys77 and His110, but not Tyr48, acts as ... activity of AKR1B1 increased with increasing pH, with an optimum of pH 8.5. The pK a value of PGDS activity was calculated by Eqn (1) to be 7.94 ± 0.07. Nonenzymatic autod...

Ngày tải lên: 14/03/2014, 23:20

... mgÆmL )1 ) of 0.97 was used. For the isolated wild-type protein (without fusion partner), A 280 of 0.65 was used whereas, for the isolated Trp mutants, A 280 of 0.54 was used. Activation of glucokinase ... elements of the proteins were evaluated by the CD Neural Network algorithm [32]. The global confor- mational stability, as measured by thermal denaturation (5–90 °C), was de...

Ngày tải lên: 30/03/2014, 04:20

... luciferase reporter assay of the IFN-b gene promoter using the various cells treated with M-pIC. The dual luciferase reporter assay was performed as described in (A) except that poly(I-C) was added ... luciferase reporter assay were performed as described in Fig. 1B. The relative level of the IFN-b gene promoter activity was calculated as described in (A). Limited suppression of t...

Ngày tải lên: 18/02/2014, 16:20

... suggestive of a catalytic water molecule which has been replaced by the acetate moiety. The protein coordinates zinc with the N d atom of His12, one of the O d atoms of Asp15 and the N e atom of His113. His113 ... presence of a functional (i.e. zinc-containing) active site is associated with the extent of the backbone distortion of this residue, so that a certain catalyti...

Ngày tải lên: 19/02/2014, 00:20

... promoter, was con- firmed by plasmid DNA NdeI digestion. Parental plasmid pHC1, lacking the G6PD-6PGL fragment was used as a transfection control. Plasmid DNAs were purified (Plasmid Maxi Kit, Qiagen, ... determine parasitaemia, about 10 000 erythrocytes were examined and the number of infected erythrocytes was reported as percentage of the total. Stage-specific development was asses...

Ngày tải lên: 19/02/2014, 07:20

... datasets for this clustering task, with over 15 research groups submitting results in each campaign. Since the re- lease of the first datasets, this task is becoming an increasingly popular research ... of refinements are the most successful. In our ex- periments we have considered the search results associated to one individual as a set of relevant documents, and we have tested the abi...

Ngày tải lên: 20/02/2014, 09:20

... alkylation of the DNA ladder by mitoDC-81 (Fig. 3B, lane 1). Analysis of the gel prior to electrotransfer showed that the extent of alkylation was proportional to the amount of DNA present and was independent ... was independent of the size of the DNA fragment (Fig. 3A, lane 1). As mtDNA in vivo is negatively supercoiled it was also important to compare the ability of...

Ngày tải lên: 20/02/2014, 11:20