Báo cáo khoa học: Ca2+ rise within a narrow window of concentration prevents functional injury of mitochondria exposed to hypoxia ⁄reoxygenation by increasing antioxidative defence pdf

... permea- bilization of the mitochondrial membrane are involved in mitochondrial damage. Our data demonstrate that hypoxia ⁄ reoxygenation and extramitochondrial Ca 2+ cause functional damage of isolated rat liver ... Ca 2+ rise within a narrow window of concentration prevents functional injury of mitochondria exposed to hypoxia ⁄ reoxygenation by increa...

Ngày tải lên: 30/03/2014, 11:20

... (25 kDa), ovalbumin (43 kDa), BSA (67 kDa) and Blue Dextran (2000 kDa). BSA was removed from the standards at pH 4.0 because of oligomerization, which leads to an abnormal molecular mass value. Building ... they are available for self-interaction. This may lead to oligomerization or aggregation. At last, we provide data on the usefulness of H ⁄ D-exchange mea- surements to analyze i...

Ngày tải lên: 16/02/2014, 09:20

... with H3K4me2 epitopes in enzymatic assays. By contrast, in the investigation by Patel et al. [35], MALDI-TOF MS was used to quantitate the degree of H3K4 meth- ylation, which shows an accumulation of the ... interaction to the KIX or CREB-binding domain of CBP [31]. The KIX domain of CBP is a structural platform that is capable of binding several different families of tr...

Ngày tải lên: 16/02/2014, 14:20

... reports that there is protease contamination of some commercially available bacitracin preparations [31], we were loathe to fractionate the material to ensure that there was no prote- ase contamination, ... rhodanese was also decreased by bacitracin. The decrease in the noncatalyzed rate (31%) was similar to that of the PDI-catalyzed reaction (a 32% decrease). These results Fig....

Ngày tải lên: 16/02/2014, 14:20

... a- galactosidase A variants (causing another lysosomal storage disease) were shown to be folding and trafficking mutants [16] before this was explored as a possibility in GD. Galactose administration increased ... administration increased Q279E a- galactosidase A residual activity in patient derived cells; thus, galactose was demonstrated to be first active-site-directed pharmacolog...

Ngày tải lên: 18/02/2014, 16:20

... molecular mass value of HAD was measured; instead of an adduct increase, or no change, the value had unexpectedly decreased from 22 417.0 Da to 22 413.2 Da, a loss of 4 Da. MS ⁄ MS of these molecular ... from deamidation of RNase A over increasing time periods. Deamidation of any one of the 17 Asn and Gln sites of RNase A produces a 1 Da increase in the mass, –CO-N...

Ngày tải lên: 18/02/2014, 16:20

... cellular proliferation in histological material is a valuable component of conventional histopathologi- cal analysis and may be of major prognostic import- ance [56]. Proliferation in immunohistochemical sections ... reduction assay A stock solution of PMA (Sigma) at 160 lm was prepared in dimethylsulfoxide, and stored at )20 °C in the dark. To stimulate HL60 cells to differenti...

Ngày tải lên: 19/02/2014, 00:20

... Ichimiya S, Nakagawara A, Sakuma Y, Kimura S, Ikeda T, Satoh M, Takahashi N, Sato N & Mori M (2000) p73: structure and function. Pathol Int 50, 589–593. 23 Levrero M, De Laurenzi V, Costanzo A, ... DNA binding to DNA treatment with cisplatin and the ability of the particular p53 target site to accommodate the cisplatin IACs. The higher the probability of formation of the cispl...

Ngày tải lên: 19/02/2014, 05:20

... amide oxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similar to what is observed in VPRK. Both PRK and VPRK have calcium bound at Ca3. SPRK also ... Bajorath J, Muller A, Raghunathan S, Singh TP, Hinrichs W & Saenger W (1991) Inhibition of pro- teinase K by methoxysuccinyl-Ala-Ala-Pro-Ala-chloro- methyl ketone: an x-ray study at...

Ngày tải lên: 19/02/2014, 07:20

... this alignment that all PAS-annotated A. thaliana proteins also contain a PAC motif, and conversely that all PAC- annotated A. thaliana proteins contain a PAS domain. Therefore, in the case of A. ... of all A. thaliana sequences that are either annotated as a PFAM PAS domain or as a PFAM PAC motif. Regions of sequences that have an amino acid sequence similarity >35%, are...

Ngày tải lên: 19/02/2014, 12:20