Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

... 2617 Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA Andrey Kropotov 1,2,3 , ... 37–45. 18 Kropotov AV, Grudinkin PS, Pleskach NM, Gavrilov BA, Tomilin NV & Zhivotovsky B (2004) Downregulation of peroxiredo...

Ngày tải lên: 30/03/2014, 11:20

... considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is envis- aged. This is ... dispute over the metal content of the RNR of C. ammoniagenes is resolved. In the present study, our strategy was to establish the homologous expression o...

Ngày tải lên: 15/02/2014, 01:20

... maintaining the geometry of the active site through the contact among subunits [31]. Neighbouring subunits are necessary to prevent water penetration into the active site and provide rigidity to the ... (short-term) of poikilotherms to temperature very often leads to chan- ges in two main traits of some metabolic enzymes: quantitative and qualitative. The quantit...

Ngày tải lên: 19/02/2014, 02:20

... content by flow cytometry revealed that the inhibition of cell growth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig. 3B). In a total of seven separate ... receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modified proteins. These results a...

Ngày tải lên: 19/02/2014, 16:20

... Ca 2+ independent. Considering all the different pathways reported for the activation of Ras and ERK1 ⁄ 2 downstream of GPCRs, Ras-GRF1 could be one of possibly several GEFs involved in the activation of Ras and subse- quently ... Research Council of Norway, The Novo Nor- disk Foundation, Anders Jahre’s Foundation for the Promotion of Science, The Blix Family Found...

Ngày tải lên: 19/02/2014, 18:20

... expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showed proliferation of human T-cells bearing Vb1, V b2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differ- ences in the ... of several pathologies and because of the potential use of the toxins as biological weapons. Alteration of their MHC and TCR binding capacity by site direct...

Ngày tải lên: 07/03/2014, 16:20

... consist- ing of the ecdysone receptor (EcR) and ultraspiracle (USP) [7,8]. The 20E–receptor complex directly induces several early genes and the products of these genes activate late effector genes ... of the brains and analyzed by RT-PCR to assess the spatial distribution of gene expression. (C) RT-PCR analysis of EcR expression using RNA from the anterio...

Ngày tải lên: 16/03/2014, 13:20

... e determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic DNA ... fractionated on a 2% (w /v) agarose gel. The remainder of the PCR-amplified DNA was ligated into TOPO-Blunt TM vectorandusedtotransformTOP10cells (Invitr...

Ngày tải lên: 16/03/2014, 18:20

... for the negative controls. The resolution varied between 1 and 5 lmÆpixel )1 depending on the size of the section of the image. The Adobe photoshop program was used to produce merged images in the ... testis, shows expression of STK33 in lung and heart. However, GeneCards expression data for STK33 are close to the background level, which is fitting to the...

Ngày tải lên: 16/03/2014, 23:20

... the qor genes and their flanking regions. The plasmid pBG3a was digested with XhoIandDraIII to remove nptII. After the removal of the 3¢ overhang and the filling of the 5¢ overhang of the plasmid ... that cleaves 364-bp upstream of the start codon of qorM ,and DraIII, that cleaves 8-bp downstream of the stop codon of qorL. After removing the 3¢ overhang...

Ngày tải lên: 17/03/2014, 10:20