Báo cáo khoa học: The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea pot

... fold of PsHO1 including position of the bound heme molecule. (B) Close up of heme bind- ing pocket. An asterisk indicates the space above the plane of the heme molecule, that is present in plant, ... cDNA sequences of PsHO1 indicating the location of intron-exon boundaries. The posi- tion of the mutation in pcd1 causing premature chain termination is in...

Ngày tải lên: 30/03/2014, 11:20

... remains uncer- tain. To summarize, analysis of individual amino acid substitutions, coupled with molecular modeling of protein structure, indicate that b-strand 7 of the a-crystallin domain is ... the remaining b-strands, with the exception of strand 6, are arranged in two antiparallel beta sheets within the a-crystallin domain. The inter- face between monomers of a di...

Ngày tải lên: 07/03/2014, 12:20

... found in the FAD49 PX domain, as in many PX domains, suggesting that the PX domain could bind to an SH3 domain of FAD49. To test whether the PX domain could interact with an SH3 domain in FAD49, ... lipid-binding activity of the PX domain in the resting state; phosphorylation of p47 phox releases the binding, resulting in the active state, i.e. open conformation...

Ngày tải lên: 16/03/2014, 04:20

... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... catalytic domain. Here, the data allowed identification of the core of the pro- tein in the N state and the evolution of the overall structure of the prote...

Ngày tải lên: 16/02/2014, 09:20

... and ERb was increased in both ERE1 and ERE2 in the presence of E2. Interestingly, the kinetics of E2-dependent binding of ERa and ERb to both ERE1 and ERE2 are differ- ent. The binding of ERa is ... absence of E2, and this binding was increased in the presence of E2 (Fig. 5A,B; compare 0 h and 6–8 h time points). These differences in the kinetic profiles of bind...

Ngày tải lên: 18/02/2014, 14:20

... process of the cotranslational insertion of the D1 protein into the thylakoid membrane, then one could imagine that the absence of PG leads to a drop in translational elongation of the D1 protein. Indeed anionic ... considerable decrease in the rate of translation of the D1 and apoCP47 subunits of the PSII core. The marginal amounts of PSII cores produced...

Ngày tải lên: 07/03/2014, 15:20

... to clarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium F-ATP ... c 10 complex in the recombinant c ring preparations suggests that the insertion of the last c subunit forms the limiting step in the assembly process of a...

Ngày tải lên: 07/03/2014, 21:20

... demonstrated that the serine protease inhibitor a-1 antitrypsin binds preprohepcidin within the cell during maturation. Furthermore, a-1 antitrypsin binds prohepcidin significantly in the plasma. This ... hepcidin is with the iron exporter molecule ferroportin. We were interested in whether we could identify new protein– protein interactions of preprohepcidin, prohepcidin and hep...

Ngày tải lên: 16/03/2014, 01:20

... two proteins inhibits the antiapoptotic activity of Bcl-2 by initiating the release of cytochrome c from mitochon- dria into the cytosol and increasing the activities of caspase 3 and caspase 7, resulting ... [44]. The interaction between Beclin 1 and its binding partners regulates the initial steps of auto- phagy. Beclin 1 also possesses a so-called BH3 domain (amino acid...

Ngày tải lên: 28/03/2014, 23:20

... [7,52,57]. Nevertheless, independently of the resulting effect of the thermal stress, all mechanisms point to the involve- ment of oxidative stress in the early stages of symbiosis breakdown. In fact, ... strengthens the involvement of a caspase-like activity, in animal tissue of A. viridis, avoiding interference by generic proteas- es. In the two animal cell l...

Ngày tải lên: 30/03/2014, 10:20