Báo cáo khoa học: The molecular basis of heme oxygenase deﬁciency in the pcd1 mutant of pea pot

... fold of PsHO1 includingposition of the bound heme molecule. (B) Close up of heme bind-ing pocket. An asterisk indicates the space above the plane of the heme molecule, that is present in plant, ... cDNA sequences of PsHO1 indicating the location of intron-exon boundaries. The posi-tion of the mutation in pcd1 causing premature chain termination isindicated. Numbers refer to the ﬁrst and ... kDa in wild-typeseedlings. This band was completely absent in pcd1 seedlings consistent with the presence of the prematuretranslation termination codon in the mutant gene. The pcd2 mutant of pea...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... remains uncer-tain.To summarize, analysis of individual amino acidsubstitutions, coupled with molecular modeling of protein structure, indicate that b-strand 7 of the a-crystallin domain is ... the remaining b-strands, with the exception of strand 6, are arranged in two antiparallelbeta sheets within the a-crystallin domain. The inter-face between monomers of a dimer involves interactionbetween ... directly in the dimer–dimer interface. As a result of the spatial dis-position of monomers and their b-strand elements in the a-crystallin domain, the amino acid substitutionF112R introduces...

Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx

... found in the FAD49 PXdomain, as in many PX domains, suggesting that the PX domain could bind to an SH3 domain of FAD49.To test whether the PX domain could interact with anSH3 domain in FAD49, ... lipid-binding activity of the PX domain in the resting state; phosphorylation of p47phoxreleases the binding, resulting in the active state,i.e. open conformation [25,35,36].As FAD49 contains ... SH3domains. In fact, it has been reported that the PXdomain of p47phoxbinds intramolecularly to the SH3domain in the same protein, and that this intramolecu-lar interaction suppresses the lipid-binding...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chaincorresponding to the catalytic domain (C domain);and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... catalytic domain. Here, the data allowed identiﬁcation of the core of the pro-tein in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution ... hairpin TH8–TH9, which is responsiblefor the insertion of the T domain into the membrane. In the absence of the lipid bilayer, this hydrophobic part of the domain self-assembles, leadingto the...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

... and ERb wasincreased in both ERE1 and ERE2 in the presence of E2. Interestingly, the kinetics of E2-dependent binding of ERa and ERb to both ERE1 and ERE2 are differ-ent. The binding of ERa is ... absence of E2, and this binding wasincreased in the presence of E2 (Fig. 5A,B; compare0 h and 6–8 h time points). These differences in the kinetic proﬁles of binding of ERa and ERb suggestthat they ... wasobserved in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERbwas increased in both ERE1 and ERE2 of the HOXC13 promoter (Fig. 5A, lanes 1–4). The levels of E2-induced...

Báo cáo khóa học: A single mutation that causes phosphatidylglycerol deﬁciency impairs synthesis of photosystem II cores in Chlamydomonas reinhardtii pdf

... process of the cotranslationalinsertion of the D1 protein into the thylakoid membrane,then one could imagine that the absence of PG leads to adrop in translational elongation of the D1 protein. Indeedanionic ... considerabledecrease in the rate of translation of the D1 and apoCP47subunits of the PSII core. The marginal amounts of PSIIcores produced in these strains can not associate in LHCII–PSII supercomplexes. These ... content.Translation initiation of the psbAmRNA is not affected in the mf2strain The dramatic decrease in D1 synthesis in mf1 and mf2 strainsdid not correlate with any signiﬁcant changes in the accumulation of...

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... toclarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium F-ATP ... c10complex in the recombinant c ringpreparations suggests that the insertion of the last csubunit forms the limiting step in the assembly process of a functional oligomer.Upon closer inspection, the ... the size of the ring isnot changed by massive overexpression of subunit c in the E. coli host cells, indicating that intrinsic fea-tures of the monomeric unit determine the number of subunits...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... demonstrated that the serine protease inhibitor a-1 antitrypsin binds preprohepcidin within the cell during maturation. Furthermore, a-1 antitrypsin binds prohepcidinsigniﬁcantly in the plasma. This ... hepcidin iswith the iron exporter molecule ferroportin. We wereinterested in whether we could identify new protein–protein interactions of preprohepcidin, prohepcidinand hepcidin in vivo. For these ... explain the presence of prohormone in the circulation, as well as the post-translational regulation of the mature hormone level in the blood. In addition, the lack of cleavageprotection in patients...

Báo cáo khoa học: Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis pdf

... twoproteins inhibits the antiapoptotic activity of Bcl-2 byinitiating the release of cytochrome c from mitochon-dria into the cytosol and increasing the activities of caspase 3 and caspase 7, resulting ... [44]. The interaction between Beclin 1 andits binding partners regulates the initial steps of auto-phagy. Beclin 1 also possesses a so-called BH3 domain(amino acids 114–123) that mediates the interactionwith ... and other close Bcl-2 homologs, such asBcl-xL and Mcl-1 [45]. Mutation of the BH3 domain of Beclin 1 or of the BH3 receptor domain of Bcl-2 ⁄Bcl-xL abolishes their capacity to inhibit Beclin1-dependent...

Báo cáo khoa học: Oxidative stress and apoptotic events during thermal stress in the symbiotic sea anemone, Anemonia viridis potx

... [7,52,57].Nevertheless, independently of the resulting effect of the thermal stress, all mechanisms point to the involve-ment of oxidative stress in the early stages of symbiosisbreakdown. In fact, ... strengthens the involvement of a caspase-like activity, in animal tissue of A. viridis, avoiding interference by generic proteas-es. In the two animal cell layers, the effect of the twoinhibitors ... and the small subunit ﬁt between the two domains.Residues boxed are the component of substrate binding site. Asterisks indicate the His and Cys residues of the putative active site located in the...

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