... 2553–2562 ª 2006 The Authors Journal compilation ª 2006 FEBS Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3 Isei Tanida, Yu-shin ... it is unclear whether murine Atg8L modification is mediated by human Atg4B, Atg7 and Atg3. Expression of Atg8L in HEK293 cells led to clea-vage of its C-terminus. In vitro, the C-terminus of Atg8L ... of Atg8L-II occurred (Fig. 5, lane 2).DiscussionHere, we have shown that Atg8L is a substrate of reac-tions mediated by human Atg4B, Atg7, and Atg3. Wefound that the C-terminus of Atg8L is...