Báo cáo khoa học: Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms docx

... 25 39 Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix -PAS- A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated ... Fe(II) and Fe(II)–CO complexes of the bHLH -PAS- A domain of NPAS2 are shown inY. Mukaiyama et al. Characterization of bHLH -PAS- A of NPAS2FEBS Journal 27 3 (20 06) 25 28 25 39 ª 20 06 The Authors Journal ... Kineticstudies of DNA cleavage reactions catalyzed by an Characterization of bHLH -PAS- A of NPAS2 Y. Mukaiyama et al. 25 38 FEBS Journal 27 3 (20 06) 25 28 25 39 ª 20 06 The Authors Journal compilation ª 20 06...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... annotation of the DNAsequences ﬂanking the Jannaschia sp. CCS1 HYDJsrevealed an ORF encoding a putative allantoate amido-hydrolase, which is part of the urate catabolic pathwayin many organisms ... (1988)Stereo- and substrate-speciﬁcity of a d-hydantoinase and a d-N-carbamyl amino acid amidohydrolase of Arthrobacter crystallopoietes AM 2. Enzyme MicrobTechnol 10, 618– 625 . 12 Ogawa J & Shimizu ... thermosta-bility. J Bacteriol 185, 4038–4049. 20 Nanba H, Yajima K, Takano M, Yamada Y, IkenakaY & Takahashi S (1997) Process for producing d-N-car-bamoyl -a- amino acid. European Patent ApplicationNo....

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... Surfacearea calculations for the pentamer give a total surfacearea of  81 000 A ˚ 2 with 30% ( 24 000 A ˚ 2 ) as con-tact area. Thus, a large amount of the available surfacearea of the molecule ... vector between the Kpn1 and Nco1 sites using a forwardoligomer 5¢-CTTTATTTTCAGGGCGCCATGAAGCGCGCAAGACCGTCTGAA-3¢ and a reverse oligomer 5¢-AGCTCGAATTCG GATCCGGTACCTCAGAAGGTAGACAGCAGAACC-3¢. For ... Mol Biol 22 8, 22 0 24 2. 27 Anand K, Pal D & Hilgenfeld R (20 02) An overview on 2- methyl -2, 4-pentanediol in crystallization and in crys-tals of biological macromolecules. Acta Crystallogr DBiol...

Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

... (5¢-caGGATCCaagatagttgtgataaccga-3¢ and 5¢-caCTCGAGttagaacttattgacttggaa-3¢), wherecapital letters indicate the BamHI or XhoI restriction sites. The PCR was performed with the following parameters: (a) ... EhPGDH (Arg217, Asp241 and Lys263), but Glu269 was substituted with anuncharged amino acid Thr245 (in E. histolytica), similarlyto B. thetaiotaomicron PGDH (Ala253) and E. caudatumPGDH (Asn265), ... inEhPGDH (Arg55, Ser56, Asn1 02 and Asn2 72) . A substi-tution of Gln2 72 to Asn found in EhPGDH was also sharedby PGDH from E. caudatum and B. thetaiotaomicron.Arg 62/ Lys 62, which interacts with the...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... primer, 5¢-GAGAGAGGATCCATATGACAAAAACCGAGTTAC-3¢, NdeIsite; antisense primer, 5¢-GAGAGAAAGCTTCAAAACTGGGACAGTTG-3¢, HindIII site. The same method was used to amplify the codingsequence for the E.coliMGST, ... puriﬁcation,functional characterization, and projection structuredetermination. J Biol Chem 27 8, 22 199 22 209. 23 Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E,Nakamura Y, Miyajima N, Hirosawa M, Sugiura M,Sasamoto ... to the nucleo-tide sequence 10 655–11 080 in the E. coli genome with a GTG start. Sense primer, 5¢-GAGAGACATATGCCATCGGCCATTTTAAAG-3¢; antisense primer, 5¢ -GAGAGAAAGCTTCTAACGCAGGGAGAAAAC-3¢....

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... characteristics of leishmania AK2 incomparison with its mammalian counterparts and itsabsolute need of the enzyme for growth and survival of the parasite within host macrophages are required to allow the ... of AK in maintenance and regeneration of intracellular ATP levels as shown inother organisms [7] partial reversal of antileishmanial action of Ap5 A by ADP indicate that the enzyme might also ... Mannheim).AK assayEnzyme activity was assayed in the forward direction by the coupled reactions system [24 ] adding different concentra-tions of ATP and AMP as the substrates. The rate of NADH...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... region of the hazelnut LOX cDNA was ampliﬁedusing the following primers: 5¢-AAGATGAAACGTGAGACGG-3¢ (CA1); 5¢-GATGACATCTCCATGGAATAC-3¢ (CA2). The 3¢ region of the hazelnut LOX cDNAwas ampliﬁed ... Hazelnut seeds contain reasonable levels of linoleic and linolenic acid (about 10 and 0 .2% , respectively, of the total fatty acids) and hexanal and nonanal are among the most abundant volatile compounds ... with the following oligos: 5¢-GTATTCCATGGAGATGTCATC-3¢ (CA5); 5¢-AAAGTTTTTAGATTGAGACACTATTGGGAATT-3¢ (CA4). Actin cDNA wasampliﬁed using the following primers: 5¢-TGGTGTTATGGTTGGTAT-3¢ (ACTFOR);...

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... of the cognate tRNA (Eqn 2) .amino acid þ ATP $ aminoacyl-AMP + pyrophosphateð1Þamino acyl-AMP + tRNAaa$ AMP + aminoacyl tRNAaa 2 Most aaRS catalyse the formation of the aminoacyl-AMP ... the Institut Pasteur collection as the template in the presence of synthetic oligonucleotidespairs.Primer 1: 5¢-AAGAAGAAGCATATGTCACCGTGCCCGACCAGCTG-3¢Primer 2: 5¢-AAGAAGAAGCATATGACCGCCACGGAAACAGTCCGG-3¢ The ... of Mt-GluRS reveals that both the monomer and the dimer are catalyticallyAbbreviationsaaRS, aminoacyl–tRNA synthetases; ALA, d-amino levulinic acid; ALAS, d-amino levulinic acid synthase; ArgRS, arginyl–tRNA...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... lac1 PLAC1F(5¢-AGCTTTCATTCCCAGTGATTG-3¢)andPLAC1R(5¢-AACGAGCTCAAGTACAAATGACT-3¢) were designed accordingto our cloned cDNA (GenBank Accession No. AY2490 52) . The PCR cycle programme was: ... egg stage (day 21 ), elongation stage (day 22 ), and mature fruiting body (day 23 ). The results of RT-PCRanalysis of gene transcription in fungal mycelia grown onrice-straw and analysed at various ... mL of distilled water. After draining off anyremaining free water, the straw was mixed with lime(15 g) and wheat bran (15 g) and the material distributedinto cellophane bags and autoclaved at...

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... P763(5¢-TTCTCGAGACGCGTTATCGATAGAGAAATGTTCTGGC-3¢) and digested the PCR product with EcoRI and XhoI. The insert was synthesized with primers P764(5¢-AACTCGAGGCTAGTCTGCAGGAGCTCAAGCTTTCTAGAGAATTCA-3¢)andP765(5¢-TGAATTCTCTAGAAAGCTTGAGCTCCTGCAGACTAGCCTCGAGTT-3¢). ... E3 and E18 (5¢-ACAAAGAAGCCCTGTACTGAATGGTCTCAG-3¢), and exons 13 and 14 by primers E16 (5¢-CATTCAGTACAGGGCTTCTTTGTGTCTGTG-3¢)andE17(5¢-AAGAATTCTCATCCCCCCAGCCACAG-3¢). The full se-quence was ... second halfwas ampliﬁed by primers E 22 (5¢-GTGGGACATCAAAACGGATTCTGGGGGTCTG-3¢)andE23(5¢-CTTGCTTTTTTTCTCTCCCCACACGGTGAAC-3¢). PrimersE20 and E 22 contained two mutations eliminating transla-tion...

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