Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx
... ª 2006 The Authors Journal compilation ª 2006 FEBS 2527 Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL 4 Alejandra Sa ´ enz 1, *, ... examine the interaction of KL 4 with POPG and PA as well as the effect of these lipids on the physical properties of the membrane. Effect...
Ngày tải lên: 30/03/2014, 11:20
... (a 1 /a 2 )andab regulatory subunit [9]. The enzymatic activity of all MATs studied depends on the redox state of the sulfhydryl moieties on the cysteine residues in the amino acid sequence of the protein ... one-tenth of the activity displayed by the wild type. However, the ability of C92S, C106S and C47S to cleave PPP i remained high, and in the case o...
Ngày tải lên: 08/03/2014, 08:20
... and was absent in the spectrum of the oxidized sample (data not shown). The axial signal with g^ = 1.93 and gk = 2.047 was assigned to a [4Fe–4S] cluster. These findings, together with those of ... amounts of MM0632. In contrast to the normal SODs, an excess of the protein from M. mazei caused reoxidation of reduced cytochrome c (Fig. 4). The enzymic activity of M...
Ngày tải lên: 28/03/2014, 23:20
Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf
... measuring the radioactivity of tritium, as described in Materials and methods (In the figure, the y-axis represents the cpm values of pellet). Black and hatched bars represent the radio- activities of ... expressed as a per- centage of the maximum releasable Ca 2+ using the fluorescence and the radioactivity of 45 Ca 2+ , and were compared with those caused by...
Ngày tải lên: 29/03/2014, 23:20
Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... with other cystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by k ass values in the range of 10 6 )10 7 m )1 , and high k diss values in the range of 10 )3 to ... rather non- selective inhibitors. An interesting feature of cystatin F is the 100-fold stronger inhibition of cathepsins F, L, and V than of cathepsin S. Cathepsin S is...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... min of reaction time, the yields of the trimer and the pentamer com- pared to the dimer were 34% and 30% for the wild- type, but 66% and 47% for the D392N mutant (Fig. 5B). Oligosaccharide synthesis ... adjusting the concentration of the enzymes to yield similar activity, the hydrolytic activities of the wild-type protein and of the D392N mutant were a...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc
... I in the presence of CI. More precisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig. 3B). The centers ... affinity of CI to the two operators, and the mecha- nism of action of CI. In the present study, we report the purification of /11 CI to near homogeneity...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf
... was positive for the Ghana, Png and Uganda sequences. Position 1278 was the only position of the alignment where both a-andb-O-GlcNAchavebeenpredictedintheGhana,Png and Uganda sequences. The Thr1503 ... enriched in the 19-kDa C-terminal fragments of MSP-1 and in the erythrocytic, GPI-linked CD59. The Triton X-100 soluble fractions of the gradient contained the bu...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... manner, the entropic contributions of nucleotide binding and the coupled oligomerization show opposing signs. In the case of hGBP1, release of water from the nucleotide binding pocket and the pro- tein ... by hGBP5, and the enzymatic hydrolysis of GTP leads to GDP rather than GMP. The nucleotide affinities of hGBP5 are grossly similar to those of hGBP1, but ther...
Ngày tải lên: 15/03/2014, 10:20
Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf
... Mge1p were used in the same concentration as DnaJ and GrpE. Specific activity values (upper) and ratio of the Hsp70 ATPase activity in the presence of the speci- fied ligands to the activity without ... that of DnaK. The twofold reduction of the K SV values estimated for mtHsp70 both in the absence and the presence of ATP (Table 2) supports the existenc...
Ngày tải lên: 16/03/2014, 22:20