Báo cáo khoa học: Mutant recombinant serpins as highly specific inhibitors of human kallikrein 14 ppt

... scissile bond of the RCL and obtained specific inhibitors towards hK2. Based on this approach, we developed highly specific recombinant inhibitors of human kallikrein 14 (hK14), a protease correlated ... FEBS Mutant recombinant serpins as highly specific inhibitors of human kallikrein 14 Loyse M. Felber 1,2 , Christoph Ku ¨ ndig 1,2 , Carla A. Borgon ˜...

Ngày tải lên: 30/03/2014, 11:20

... ‘pseudo-dyad’ of J-ACTX-Hv1c is topologically similar to that of ChTx (Fig. 1G), although the overlay is not as good as with the dyad of the K V channel blockers BgK and agitoxin 2 [4]. How- ever, as we ... hyperpolarization [12]. It has been suggested that activators and blockers of BK Ca channels may have application as neuroprotectants or as therapeutics in certain diseas...

Ngày tải lên: 16/03/2014, 06:20

... than increased, the expression levels of the recombinant protein (data not shown). Similar, negative effects of proteasome inhibitors on recombinant protein expression (reduction of luciferase and ... 44/750 and 1/750 variants. A decrease of more than 80-fold in the secretion of recombinant protein was associated with the deletion of the C-terminal part(s) of the protein...

Ngày tải lên: 07/03/2014, 15:20

... increase in A s or a large increase in A ss occurred, depending on the isoform. The large increase in A s was occasionally associated with a smaller increase in A ss . The average value of the fast ... tested VGSC isoforms. Results Screening of peptides for robustness of the slowing of the inactivation process both in mammalian isoforms and in one insect isoform As only a limited am...

Ngày tải lên: 15/03/2014, 10:20

... negative Ras expression plasmid, in which the V12 mutant of H-Ras is driven by the RSV promoter were provided by X. Fang (MD Andersen Cancer Institute, Houston, TX, USA) [47]. Cell culture, plasmid ... Each assay included a standard curve of five serial dilutions of a known concentration of cDNA and a non- template control. All assays were performed in triplicates. The expression lev...

Ngày tải lên: 30/03/2014, 16:20

... inhibitor, as a result of its inhibitory effect on glucose hexokinase [16,20,21], it was suspected that the observed decrease in reporter activity was caused by a depletion of ATP in these cells. As ... GSL of the NCCIT cell was Gb3 (Fig. 6B). After 2DG treatment, the level of Gb3 markedly decreased, which was followed by an accu- mulation of LacCer and the appearance of anoth...

Ngày tải lên: 30/03/2014, 01:20

... biological profile of genes having species -specific LIRs. We have three sets of test genes, which include the human orthologs having human -specific LIRs, the human orthologs having human- and chimpanzee -specific ... basically constructed by dinucleotide repeats. In the case where TG + TA + CA > 80% of the arm of an LIR, it was considered as TG ⁄ CA-rich; in the case whe...

Ngày tải lên: 07/03/2014, 00:20

... because of the complete lack of annotated data. DLD09’s insight was to make use of negative polarity items (NPIs), which are words or phrases that tend to occur only in negative con- texts. The reason ... (a) “NPI context” was defined as the part of the sentence to the left of the NPI up to the first comma, semi-colon or beginning of sentence; (b) to encour- age the discovery of...

Ngày tải lên: 17/03/2014, 00:20

... example as markers of education, but socio- linguistic issues are beyond the scope of this paper. There is no satisfactory synchronic reason for the existence of the gaps. The grouping of gaps ... removing lemmas with low attestation. We calculated the probability of the 1sg inflection by dividing the number of 1sg forms by the total number of non-past forms. The subset...

Ngày tải lên: 17/03/2014, 04:20

... distribution of bovine tryptases In order to ascertain the expression of one or both tryptase isoforms (see Results) in different bovine tissues, tryptase cDNAs were prepared as described above from mRNAs isolated ... feature of tryptases seems to be their occurrence as a multigene family: in humans, at least four homologous tryptase cDNAs (tryptases a and bI–III) have been isolated...

Ngày tải lên: 17/03/2014, 09:20