Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx

... shown that their N-terminus modulates their activity [36,37]. The current hypothesis involves domain–domain interac- tions between the N-terminus and other domains of the ATPase. Indeed, in vitro ... N-terminus of the ATPase. To further study the role of the Ccc2 N-terminus in the overall function of the ATPase in vivo, we engi- neered DMBDCcc2 (Fig. 1E), a p...

Ngày tải lên: 23/03/2014, 05:22

... Bacteriophage MS2 stability FEBS Journal 273 (2006) 1463–1475 ª 2006 The Authors Journal compilation ª 2006 FEBS 1475 Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage ... understood, and we have investigated these questions in this work. We sought to determine the role of protein–RNA and protein–prot...

Ngày tải lên: 30/03/2014, 11:20

... of the trypsin-sensitive region situated in the linker domain of Abcb1a. Fol- lowing trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation pro- ducts of ... E552 in NBD1 and E1197 in NBD2. To further characterize the role of these residues in catalysis, we created in Abcb1a the single-site mutants E552D, N and A...

Ngày tải lên: 07/03/2014, 06:20

... documents and find that the fea- ture vectors corresponding to some of these docu- ments (particularly the short ones) have all zeroes in them. In other words, none of the bigrams from the training ... one in which the author himself likes the movie, and in the other, the author’s cousin likes the movie. The over- generalization resulting from these “stemme...

Ngày tải lên: 17/03/2014, 04:20

... requirements of many text understand- ing applications to the problem of determining whether the meaning of a given textual assertion, termed hypothesis (H), can be inferred from the meaning of certain ... was mandatory for inference. In another 28%, references could optionally support the inference of the hypothesis. In the remaining 28%, refer- ences did not contri...

Ngày tải lên: 30/03/2014, 21:20

... performed by asking people to describe the process of getting themaelves and their husbands and children off to work in the morning. (Linde, in preparation) These "morning routines" are ... MAPS In describing apartment layouts, there is a minority strategy, used by 4% of the speakers (3 out of 72 cases of the data of Linde (1974)) describing the layo...

Ngày tải lên: 17/03/2014, 19:20

... reduced one and again the mass fitted to the sum of the two peptides ()2H + 1) containing the same cysteine residues, Cys35 and Cys83. Also, in the spec- tra of the oxidized and reduced protein the peptides containing ... found in the spectrum of the reduced protein. The mass of this peak can be assigned to the sum of two peptides containing two cystei...

Ngày tải lên: 23/03/2014, 07:20

... becomes acidic in the final step of PND [22,23]. DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and persisted until resorp- tion of the parental ... (Figs 1 and 3). According to the observation made by Lu and Wolfe [23], who used a combination strategy of 4,6-diamino-2-phenylindole (DAPI) staining for the det...

Ngày tải lên: 20/02/2014, 03:20

... anchoring the protein to the inner membrane of mitochondria [27]. Therefore, the N-ter- minal segment (containing the residues protruding into the mitochondrial matrix, the transmembrane helix and ... apo-Cu A site passing through Sco1 and Sco2 proteins (Fig. 5), but the molecular details of the role of Sco proteins in the copper insertion into the Cu A site...

Ngày tải lên: 14/02/2014, 18:20

... probes 16-doxylstearic acid in the interior of the micelle (A) and Mn 2+ in the aqueous buffer (B) selectively attenuate distinct regions of VpU- cyt, reflecting the topology and the dynamics of the micelle-associ- ated ... between helix 3 and the loop were strongly reduced in the presence of the doxyl-bearing fatty acid. Mn 2+ ions quenched the cros...

Ngày tải lên: 18/02/2014, 06:20