Báo cáo khoa học: Mycobacterium tuberculosis H37Rv ESAT-6–CFP-10 complex formation confers thermodynamic and biochemical stability docx

... ESAT-6–CFP-10 complex formation confers thermodynamic and biochemical stability Akshaya K. Meher 1 , Naresh Chandra Bal 1 , Kandala V. R. Chary 2 and Ashish Arora 1 1 Molecular and Structural ... antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and...

Ngày tải lên: 30/03/2014, 11:20

... human lungs, such as Mycobacterium tuberculosis, Pseudomonas aeruginosa, Burkholderia cepa- cia and Bacillus anthracis. The genome of M. tuberculosis, the etiologic agent of tuberculosis, encodes ... concen- tration, the 70 kDa band disappeared, and two new bands of 45 and 26 kDa were seen on SDS ⁄ PAGE that corresponded to the mycobacterial Rv1771 pro- tein and the freed GST tag...

Ngày tải lên: 07/03/2014, 12:20

... Construction and purification of M. tuberculosis ClpC1 and its deletion mutants. (A) SDS ⁄ PAGE of purified full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2. (B) Full-length ClpC1 and deletion ... on ATP hydrolysis, and translocate folded and assembled complexes, as well as improperly folded and aggregated proteins for degradation by ClpP [15]. They also disaggregat...

Ngày tải lên: 23/03/2014, 06:20

... trimethylation and H3K36 dimethylation during transcription initiation and elongation phases respectively. EFs represent elongation factors. Biochemical functions of MLL K. I. Ansari and S. S. Mandal 1800 ... regulation and mixed lineage leukemia. Acknowledgements We thank Imran Hussain, Sahba Kasiri, Bishakha Shrestha, Saoni Mandal and other Mandal lab mem- bers for useful discus...

Ngày tải lên: 16/02/2014, 14:20

... high solubility and stability; and (c) foreignness to the affected host [8]. Additionally, allergens elicit an IgE response and a Keywords covalently locked conformation; EF-hand protein; protein ... conformation of Bet v 4. The introduced K25C ⁄ F60C double mutation promotes disulfide bond formation between the two anti- parallel b-strands, and thus crosslinks the first Ca 2+ -bindi...

Ngày tải lên: 06/03/2014, 11:20

... construct VP (verb phrase) from V and UP (a verb and a noun phrase), and that the other ts the rule to construct VP (verb phrase) from V. NP and PP (a verb. a noun phrase, and a prepositional phrase). ... the annotated tree. SEARCH-CANDIDATE-OF-HOUNS.sg: sg_mode: order(Z): rr_tn_sg: CANDIDATE-OF-NOUNS-t: UP-NP-TO-PNP: CANDIDATE-OF-NOUNS-Z; end_sg.SEARCH-CANDIDATE-OF-NOUNS...

Ngày tải lên: 24/03/2014, 01:21

... between the sandwiched riboflavin and H94 and residue 27 (distances b etween the planes of about 4A ˚ )[5]. In the CEOL and NORAP structures of the W27Y mutant and in all three ligand-bou nd structures ... affinity. This is believed to be due to a p -complex formation between the bound ligand and the adjacent tryptophan residue 27 [20]. In mutant proteins, namely W27Y and W27F, r...

Ngày tải lên: 30/03/2014, 15:20

... ferredoxin; Mycobacterium tuberculosis. Information available from the complete genome sequence of Mycobacterium tuberculosis [1] has promoted a wide investigation of new targets for drugs against tuberculosis [2]. ... of unbound FprA and ligand. (A) difference spectra of the complexes between FprA and NADP + (solid line) or NAD + (dashed line). (B) difference spectrum of the com...

Ngày tải lên: 08/03/2014, 23:20

... interactions, con- traindications and side effects, these are for information only and decision support capability does not exist. Systems with APACHE = Acute Physiology and Chronic Health Evaluation; ... support, in sev- eral ways. We compare the rates and types of MEs and the potential outcome of intercepted and non-intercepted errors. Materials and methods In April 2002, Univ...

Ngày tải lên: 25/10/2012, 10:39

... ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss the possibility that both could func- tion as molecular targets for the prevention and treatment of obesity and metabolic ... focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes- ity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and tre...

Ngày tải lên: 14/02/2014, 22:20