Báo cáo khoa học: Ca2+-binding allergens from olive pollen exhibit biochemical and immunological activity when expressed in stable transgenic Arabidopsis pdf

... recombin- ant allergens may offer the possibility of having available edible vaccines in the near future. In this study, two EF-hand-type Ca 2+ -binding allergens from olive pollen, Ole e 3 and ... clinical and scientific purposes, as well as for developing new ways of vaccination. Results Obtaining Arabidopsis transgenic plants containing Ole e 3 and Ole e 8 cDNAs Binar...

Ngày tải lên: 30/03/2014, 10:20

... hand, doesn’t seem to be required for actin-binding as calponin interacts with actin via its C-terminal domain [51]. Thus, with an IF protein-binding site residing in their N-terminal CH domains ... (1995) Utrophin actin binding domain: analysis of actin binding and cellular targeting. J. Cell Sci. 108, 63–71. 10. Gimona, M. & Winder, S.J. (1998) Single calponin homology domains are n...

Ngày tải lên: 07/03/2014, 15:20

... useful information. These include destina- tions and notices in transportation facilities, names of buildings and shops, explanations at sightseeing spots, and the names and prices of dishes in restau- rants. ... user specifies the beginning and ending of the character string to be recognized and translated. In Figure 3, circles on both ends of the string denote the user spec...

Ngày tải lên: 20/02/2014, 15:20

... domain fused to a DNA- binding domain (DBD) and a ligand-binding domain (LBD). The chimeric gene (transactivation domain– DBD–LBD) is expressed under the control of a con- stitutive promoter. In ... germinate and grow on the induction media for 20 days in the case of Arabidopsis and for 4 weeks in the case of tobacco, at 25 °C, under 16 h of light and 8 h of dark. Microscopy...

Ngày tải lên: 16/03/2014, 06:20

... region containing the charged acidic amino acids should produce a strain limiting the mobility of both the amino-acid-containing loops and the attached transmem- brane a-helices. These interactions ... twists in the retinal structure and changes in its environment induced by a bacteriorho- dopsin conformational change, which produces altered protein–retinal interactions [25], while th...

Ngày tải lên: 17/03/2014, 03:20

... shape. In aqueous buffer, the CD spectra of the GGN4 analogues, including the native GGN4, showed a strong negative band near 200 nm and a weak and broad band around 222 nm, indicating a predominantly random-coil ... structures in the 50% TFE/ water mixture (gray lines) was superimposed over that in 500 m M SDS micelles (black lines), by matching the backbone atoms in residues I2...

Ngày tải lên: 31/03/2014, 09:20

... 2011) doi:10.1111/j.1742-4658.2011.08043.x Starch-binding domains are noncatalytic carbohydrate-binding modules that mediate binding to granular starch. The starch-binding domains from the carbohydrate-binding module family ... 1185 Starch-binding domains in the CBM45 family – low-affinity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism Mikk...

Ngày tải lên: 06/03/2014, 00:21

... dye (attenuating both the J-band and the c-band), indicating that the dye binds in the EF-hand motif. Binding of Mg 2+ caused a decrease only in the c-band, without disturbing the J-band (Fig. 7A). ... upon binding with GDP-bound G-protein, whereas an increase in the J-band intensity was elicited upon interaction of Calnuc and GTP-bound G-protein (Fig. 8B). Interestingly, why G-prot...

Ngày tải lên: 07/03/2014, 00:20

... operon encoding a proline dehydrogenase and a proline perm- ease [23]. PutR has been suggested to facilitate the DNA binding of CRP by direct protein–protein inter- action or to induce a change in DNA ... SenR binding, EMSAs were done. For this purpose, a  100 bp fragment (containing or lacking binding site I) upstream of furS (up-furS2) was amplified from each of the correspond- ing pl...

Ngày tải lên: 07/03/2014, 10:20

... the Keywords affibody binding proteins; affinity chromatography; combinatorial protein engineering; in vivo imaging; peptide synthesis; phage display; protein chips; protein–protein interactions; selection; ... immunoglobu- lin-binding [Fc binding (IgG) and Fab binding (VHIII)] bacterial receptin have, for several decades, been widely used for the detection and purification of antibodies...

Ngày tải lên: 23/03/2014, 07:20