Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt
Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt
... compilation ª 2006 FEBS 4209 The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme Steffen Kutter 1 , Georg ... July 2006) doi:10.1111/j.1742-4658.2006.05415.x The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis has been determined...
Ngày tải lên: 30/03/2014, 10:20
Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt
... near the limit of detection for this interaction, as successive dilution of the probe soon led to loss of sig- nal. The low yields of the AP probe and of nucleolin, together with the inevitable ... One way to understand these events might be to identify the extracellular ligands of the RPTPs in the neuromuscular system. For example, it is known that PTPd and...
Ngày tải lên: 19/02/2014, 05:20
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... The Authors Journal compilation ª 2006 FEBS (e) The reaction mixture is accessible. This allows the synthesis of proteins in the presence of other pro- teins provided in excess at the start of ... of the corres- ponding uniformly labelled sample. Not a single case of complete cross-peak overlap was encountered in the case of the C-terminal domain of the s sub...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... behavior of the minidomain may influence the state of the N-domain and may therefore modify the efficiency of the decod- ing process. To verify this hypothesis, we generated a series of mutant forms of ... However, these structures do not show the coordinates of the atoms in Fig. 6. Superposition of the representative NMR open conformer of the C-domain (red a...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf
... Interest- ingly, the high sequence homology of the N-terminal portion does not lead to conserved 3D structures for this section of the molecules because of polarity differences of the few varying ... responsible for cleavage of the leader peptide after the double glycine motif [16,17]. This cleavage occurs on the cytoplasmic side of the membrane during expo...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... and cofactor binding of EcIPDC. The enzymatic conversion of all substrates studied in this work (pyruvate, the native substrate of PDC, benzoylformate, the native substrate of benzoylformate decarboxylase ... present case. The analysis of the kinetic constants of the 4-substituted benzoylformates as substrates for EcIPDC demonstrates that the dependence of...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf
... Stereo views of the solution and crystal structures of DnaG-C. (A) Superposi- tion of the backbone atoms of residues 447–581 of the 20 NMR conformers of DnaG-C representing the NMR structure (Table ... predicted for the corresponding domain from B. stearothermophilus [10] and for the monomeric DnaG-C structure repor- ted here. These data and the uniformit...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
... at the binding site of one subunit changes the conformation of the o ther subunit, thereby completely abolishing reaction of SDTG with the second subunit. Analysis of the crystal structu re of ... are located at the end of t his helix. Conformational changes in these residues would then change the affinity for CDNB binding, which is supported by the finding that...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf
... protein. This kind of protein is exemplified by the Thermus thermophilus protein, PDB entry 2B3B [28]. The mode of binding the monosaccharide is completely different in terms of orientation of the ... a characteristic of this family of proteins. Conformational changes As described above, the two molecules in the asymmet- ric unit of our structure differ only sli...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf
... evolution of the primary structure of the N-terminal regions of TTR and the biological function of TTR. Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during ... efficiency of 70%. For analysis of K d values for T3 and T4 of all recombinant TTRs, analyses for chicken TTR purified from serum were always deter-...
Ngày tải lên: 23/03/2014, 10:21