Báo cáo khoa học: A kinetic study of a ternary cycle between adenine nucleotides pptx

... presence of an smallamount of ADP and AMP due to a contamination of ATP and NADH standard solutions (data not shown).It can be seen that at the end of the reaction, peakscorresponding to ATP and ADP ... Authors Journal compilation ª 2006 FEBS 3613 A kinetic study of a ternary cycle between adenine nucleotides Edelmira Valero1, Ramo´n Varo´n1and Francisco Garcı a- Carmona21 Departamento de ... constants for a ﬁxed adenylate energy charge value and viceversa.AbbreviationsACS, S-acetyl coenzyme A synthetase; AEC, adenylate energy charge; AK, adenylate kinase; LDH,L-lactate dehydrogenase;...

Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... CT ex114 aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1IINP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... stable cys-teine protease from the latex of Ervatamia coronaria.Biosci Biotechnol Biochem 62, 1947–1955.15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M,Jagannadham MV & Dattagupta ... proteases [Erv -A, -B and –C (isolated from thelatex of Ervatamia coronaria in our laboratory) and papainfrom Carica papaya (Merck, Kenilworth, NJ, USA)], twoserine proteases [trypsin and chymotrypsin...

Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt

... Tat proofreading and TMAO reductaseactivity assaysThe signal peptide binding-dependent Tat proofreadingactivity of TorD can be isolated and assayed in vivo,asdescribed previously [5]. Strain ... TorD GTPase activity can be isolated by Cibacron Blue afﬁn-ity chromatography. (A) Unusual behaviour of TorD on CibacronÔBlue afﬁnity media. A sample of 0.5 mM metal afﬁnity chromatogra-phy-puriﬁed ... requirements of the classical GTPases [29,30]. Magnesium often func-tions as a phosphate ligand in canonical GTPases inaddition to a variety of proposed activities involved inthe actual hydrolysis reaction...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... 919628.36 Yamada Y (1983) Production of heat-resistantpolyphenol oxidase, Japanese patent 61115488.37 Yamada Y, Tawara Y & Yoshika H (1983) Production of heat-resistant polyphenol oxidase, Japanese ... JN, Tudela J, Varon R, Garcia-Carmona F & Garcia-Canovas F (1992) Analysis of a kinetic model for melanin biosynthesis pathway. J BiolChem 267, 3801–3810.4 Hearing VJ & Tsukamoto K ... rounds and tested for tyrosinase activity with a plate assay. In the assay plates, Trichoderma minimal med-ium [19] with 2% lactose as a carbon source, 1% potassiumphthalate as a buffering agent...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... University of Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcho-line receptor have a range of potential therapeutic applica-tions and are valuable probes ... mutational analysis has been carried out onImI, PnIA, PnIB and to a lesser extent on GID and MII.Analysis of ImI has revealed that Asp5-Pro6-Arg7 andTrp10 are important for biological activity ... (2-amino-D,L-dodecanoic acid, Laa) to eitherthe N-terminus (LaaMII) or to Asn5 (5LaaMII). TheN-terminal LaaMII was shown to have a tertiary structuresimilar to that of the native conotoxin and maintained...

Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... fraction).BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) wereloaded on to a separate gradient as mole-cular mass markers.K. Komaru et al. Novel aggregate formation of an alkaline ... hypophosphatasia patients. J Bone Miner Res13, 1827–1834.17 Cai G, Michigami T, Yamamoto T, Yasui N, SatomuraK, Yamagata M, Shima M, Nakajima S, Mushiake S,Okada S & Ozono K (1998) Analysis of ... H, Iimura T, Miyazaki H, OdaK, Shibata H, Yanagishita M, Takagi Y, Watanabe H,Shimada T & Oida S (1998) Expression of the mutant(1735T-DEL) tissue-nonspeciﬁc alkaline phosphatasegene...

Tài liệu Báo cáo khoa học: "An Empirical Study of Information Synthesis Tasks" doc

... but a good similarity metric musthave a good QARLA value: it is unlikely that a measure that cannot distinguish between manualand automatic summaries can still produce high-quality rankings of ... features of a goodsummary; then Lin and Hovy proposed a recall-based variation of BLEU, known as ROUGE. Theidea is the same: the quality of a proposed sum-mary can be calculated as a function of ... summarization task. BLEU is based in theprecision and n-gram co-ocurrence between an au-tomatic translation and a reference manual transla-tion.(Lin and Hovy, 2003) tried to apply BLEU asa...

Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx

... DCPIPassay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1. The singlemutants E1aR26 7A and E1aD27 6A and the multiplemutants E1aY28 1A/ R28 2A/ S28 3A ... 16-foldmolarexcessofdi-domainoverE1.Lane1:E1wild-type;lane2:E1wild-type + di-domain; lane 3: E1aF26 6A mutant; lane 4:E1aF26 6A mutant + di-domain; lane 5: E1aR26 7A mutant; lane 6:E1aR26 7A mutant + di-domain; lane 7: E1aD27 6A mutant; lane 8:E1aD27 6A ... Data were analysed and ﬁtted to a Michaelis–Menten curve using theSIGMA PLOTsoftware.Temperature-dependence of catalytic activityThe dependence of the catalytic activity of E1 on tempera-ture...

Tài liệu Báo cáo khoa học: The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase doc

... acids.Materials and methodsMaterialsChemicals were obtained from the Sigma-Aldrich ChemicalCo. or Merck and were of at least analytical grade or higher.Reagents were also supplied by: Amersham ... DAOCS and all mutants were puriﬁed to at least85% purity (as judged by SDS/PAGE and gel ﬁltrationanalyses) using a combination of anion-exchange and gelﬁltration chromatographies. The DNA sequences ... cosubstrateswith mutant enzymes.Mutants can be grouped into those that affect apparentKmvalues, those that affect kcatvalues and those thataffect both Kmand kcatvalues. In the case of...

Tài liệu Báo cáo khoa học: "DESIGN AND IMPLEMENTATION OF A LLXICAL DATA BASE " docx

... yet another example of the "historicist approach" typical of classical transformational generative grammar, which assumes that synchronic processes recapitulates many of the diachronic ... involving a large lexical data base is what I would call "practical requirements" and has to do with the complexity of the task of creating a lexical entry. It can roughly be viewed as a ... just a particular set of grammatical features such as category, gender, number, person, case, etc. A lexeme contains all the information shared by all the flectional forms of a given lexical...

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