Báo cáo khoa học: A kinetic study of a ternary cycle between adenine nucleotides pptx

... presence of an small amount of ADP and AMP due to a contamination of ATP and NADH standard solutions (data not shown). It can be seen that at the end of the reaction, peaks corresponding to ATP and ADP ... Authors Journal compilation ª 2006 FEBS 3613 A kinetic study of a ternary cycle between adenine nucleotides Edelmira Valero 1 , Ramo ´ n Varo ´ n 1 and Francis...

Ngày tải lên: 30/03/2014, 10:20

... CT ex 114 aa 208 aa 24 aa BamH 34 aa Start Stop Xho1 N -Pro Protease domain Protease domain CT - ex 380 aa 1 II NP 114 aa 208 aa BamH1 34 aa Start Stop Xho1 SS SS G SSS S N-Pro 356 aa III MSTLFII S ILLFLAS F SYAMDI S TIEYKYDKSS AWRTDEEVKEIYELWLAKHDKVY SG LVEYEKRFEIFKDNLKFIDEH NSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCG SCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHG...

Ngày tải lên: 14/02/2014, 14:20

... Tat proofreading and TMAO reductase activity assays The signal peptide binding-dependent Tat proofreading activity of TorD can be isolated and assayed in vivo,as described previously [5]. Strain ... TorD GTPase activity can be isolated by Cibacron Blue affin- ity chromatography. (A) Unusual behaviour of TorD on CibacronÔ Blue affinity media. A sample of 0.5 m M metal affinity chromatogra...

Ngày tải lên: 16/02/2014, 09:20

... 919628. 36 Yamada Y (1983) Production of heat-resistant polyphenol oxidase, Japanese patent 61115488. 37 Yamada Y, Tawara Y & Yoshika H (1983) Production of heat-resistant polyphenol oxidase, Japanese ... JN, Tudela J, Varon R, Garcia- Carmona F & Garcia-Canovas F (1992) Analysis of a kinetic model for melanin biosynthesis pathway. J Biol Chem 267, 3801–3810. 4 Hearing VJ &a...

Ngày tải lên: 19/02/2014, 06:20

... University of Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcho- line receptor have a range of potential therapeutic applica- tions and are valuable probes ... mutational analysis has been carried out on ImI, PnIA, PnIB and to a lesser extent on GID and MII. Analysis of ImI has revealed that Asp5-Pro6-Arg7 and Trp10 are important for bi...

Ngày tải lên: 19/02/2014, 12:20

... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al. Novel aggregate formation of an alkaline ... hypophosphatasia patients. J Bone Miner Res 13, 1827–1834. 17 Cai G, Michigami T, Yamamoto T, Yasui N, Satomura K, Yamagata M, Shima M, Nakajima S, Mushiake S, Okada S & O...

Ngày tải lên: 19/02/2014, 17:20

... but a good similarity metric must have a good QARLA value: it is unlikely that a measure that cannot distinguish between manual and automatic summaries can still produce high- quality rankings of ... features of a good summary; then Lin and Hovy proposed a recall- based variation of BLEU, known as ROUGE. The idea is the same: the quality of a proposed sum- mary can be ca...

Ngày tải lên: 20/02/2014, 15:20

... DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1. The single mutants E1aR26 7A and E1aD27 6A and the multiple mutants E1aY28 1A/ R28 2A/ S28 3A ... 16-fold molarexcessofdi-domainoverE1.Lane1:E1wild-type;lane2:E1 wild-type + di-domain; lane 3: E1aF26 6A mutant; lane 4: E1aF26 6A mutant + di-domain; lane 5: E1aR26 7A mu...

Ngày tải lên: 20/02/2014, 23:20

... acids. Materials and methods Materials Chemicals were obtained from the Sigma-Aldrich Chemical Co. or Merck and were of at least analytical grade or higher. Reagents were also supplied by: Amersham ... DAOCS and all mutants were purified to at least 85% purity (as judged by SDS/PAGE and gel filtration analyses) using a combination of anion-exchange and gel filtration chromatographies. The...

Ngày tải lên: 20/02/2014, 23:20

... yet another example of the "historicist approach" typical of classical transformational generative grammar, which assumes that synchronic processes recapitulates many of the diachronic ... involving a large lexical data base is what I would call "practical requirements" and has to do with the complexity of the task of creating a lexical entry. It can r...

Ngày tải lên: 22/02/2014, 09:20