Báo cáo khoa học: Charging of tRNA with non-natural amino acids at high pressure potx

... Charging of tRNA with non-natural amino acids FEBS Journal 273 (2006) 3014–3023 ª 2006 The Authors Journal compilation ª 2006 FEBS 3023 Charging of tRNA with non-natural amino acids at high pressure Malgorzata ... at high pressure. Applica- tion of MS provides evidence that charging occurs at the hydroxyl group of the 3¢-end ribose. Results TRNA...

Ngày tải lên: 30/03/2014, 10:20

... aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig. 3) suggest that excess photorespiratory ammonium was detoxified, in part, in the form of amino acids for export out of ... observations indicate that the GS ⁄ GLU1 Fd-GOGAT and GS ⁄ GLT NADH-GOGAT cycles are involved in nonphotorespi- ratory ammonium assimilation. In air, the highest glu- tamine ⁄ gl...

Ngày tải lên: 30/03/2014, 01:20

... reaction at such a high pH of 7.2 was reported to have a much higher rate, so that a considerable amount of Arg -tRNA was not left unreacted. The ratio of Arg -tRNA with the cyclic form of the Arg moiety ... ami- noacyl -tRNA. In the aminoacylation reaction of class I aaRSs with aminoacyl-AMP, the 2¢-OH group of the ribose of A76 tRNA attacks the carbonyl carbon atom...

Ngày tải lên: 18/02/2014, 11:20

... course of ChiB degradation of a chitosan with F A ¼ 0.65. The graph shows the degree of scission (a) as a function of time. The slope of the line at low a is 1.8 · 10 )3 min )1 ,andat higher ... extreme limits of the possible variation in chemical composition of an oligomer with a given DP, while the enzymatic degradation of chitosans results in oligomer fractions with...

Ngày tải lên: 07/03/2014, 16:20

... products of the ATT-template. Fig. 7. Effect of high DAPI concentration on template-directed Klenow synthesis. Denaturing gel analysis showing the effect of high DAPI concentration on the elongation of ... presence of (b) random-template, (c) AAT-template and (d) ATT-template at 2 : 1 drug/template molar ratio. The experiments were performed at 37 °C, in 100 m M NaCl and 10 m...

Ngày tải lên: 16/03/2014, 23:20

... temperature at which 50% of the protein is unfolded, and the reciprocal value of dT is a relative measure of cooperativity of the transition. A. Bekesi et al. RNA-dependent conformational states of ... an abundance of UDE mRNA as well as of domain V of 23S rRNA. These results, together with the character- istics of the two distinct conformational states, suggest putative...

Ngày tải lên: 22/03/2014, 16:21

... could be demon- strated (data not shown). These negative results indicate that 42Sp50 is either not in direct contact with the LEs or that it associates with a different region of the corresponding ... vegetal localization complexes that are formed with the corresponding signal sequences in the untranslated regions of the mRNAs and with a number of different so-called localizati...

Ngày tải lên: 23/03/2014, 03:20

... harvest of cells, the concentration of JH III in the lysate was maintained at 100 lm during subse- quent processing. For immunoprecipitation, the lysate was incubated overnight at 4 °C with rabbit ... presence of 1 lm 20-OHE or the dose range of JH III alone, and the activation ratio in relation to the solvent (EtOH) treatment calculated. As shown in Fig. 2, when the data for ac...

Ngày tải lên: 23/03/2014, 13:20

... arginine indi- cated that arginine promoted the formation of its native conformation, compared with other amino acids [10], and that arginine effectively suppressed the aggre- gation of denatured lysozyme ... form of RCG or glutathione indi- cated that prouroguanylin was converted to the native conformation more efficiently in the case of RCG, compared with glutathione. The find...

Ngày tải lên: 06/03/2014, 00:21

... cell association of uPA after release and/ or the level of activity. In fact, it has been shown previously that cell association of uPA-generating activity enhances the rate of formation of active ... experiments, 0.2% of SBTI was also incorporated in both the stacking and separating gels to prevent degradation of the incor- porated gelatin substrate by trace amounts of trypsi...

Ngày tải lên: 08/03/2014, 08:20