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Báo cáo khoa học: DG-based prediction and experimental confirmation of SYCRP1-binding sites on the Synechocystis genome pot

Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

... solutecompletely restricts concerted motions of groups on largertime-scales. The influence of DGP addition on the order parameters of RdDg is of the same magnitude as the effect of ligandbinding reported ... a final concentration of  4mM.In one sample, DGP (potassium salt) was added to a finalconcentration of 100 mM, while in the other sample KClwas added to the same concentration. The pH was ... denaturingreaction would be an effective stabilizer.This leads us to consider the possible effects of soluteaddition on the activation energy of the denaturing process and we look to the results of the...
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Báo cáo khoa học: DG-based prediction and experimental confirmation of SYCRP1-binding sites on the Synechocystis genome pot

Báo cáo khoa học: DG-based prediction and experimental confirmation of SYCRP1-binding sites on the Synechocystis genome pot

... DG-based prediction of DNA binding sites by SYCRP1FEBS Journal 275 (2008) 4786–4795 ª 2008 The Authors Journal compilation ª 2008 FEBS 4795 DG-based prediction and experimental confirmation of SYCRP1-binding ... sites. Figure 3 shows the procedure for the DDG-based prediction. The binding affinity of SYCRP1 to a fragment of 16 bp is estimated as the sum of the DDG values at each position. The window of ... shown. The posi-tions of slr1667 and slr1668 are shown at the top; the arrows rep-resent the actual binding site of the slr1667–slr1668 operon. The binding site upstream of the operon has the...
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Báo cáo khoa học:

Báo cáo khoa học: "Age Prediction in Blogs: A Study of Style, Content, and Online Behavior in Pre- and Post-Social Media Generations" ppt

... 2:841–848.Scott Nowson and Jon Oberlander. 2006. The iden-tity of bloggers: Openness and gender in personalweblogs.James W Pennebaker and Lori D Stone. 2003.Words of wisdom: language use over the life ... 1979 and 1982, and the social networking sites, MySpace and Facebook coincide with the later shifts of 1982-Figure 5: The impact of social media technologies: The arrows correspond to the years ... Introduction The evolution of the internet has changed the way that people communicate. The introduction of instant messaging, forums, social networking and blogs has made it possible for people of...
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Báo cáo khoa học:

Báo cáo khoa học: "Trace Prediction and Recovery With Unlexicalized PCFGs and Slash Features" pptx

... Co-indexation accuracy on section 23. The first column shows the category and type of the trace. If the filler category of the filler is dif-ferent from the category of the trace, it is added infront. The ... gen-erated by the parser.4.2 Co-IndexationTable 3 shows the accuracy of the parser on the co-indexation task. A co-indexation of a trace and a filler is represented by a 5-tuple consisting of the category ... of the category and the string position of the trace,as well as the category, start and end position of the filler. A co-indexation is judged correct if the treebank parse contains the same 5-tuple.For...
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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

... conditions.In FprA, modulation of ionic interactions present in the native conformation of the protein by monovalentcations has been shown to result in stabilization of acompact conformation [9]. ... for the cation stabilizedcompact state of FprA. These observations suggestthat the stabilization of a compact conformation of native FprA at low GdmCl concentration is due tointeraction of the ... purityavailable.MethodsOverexpression and purification of FprACloning, overexpression and purification of the FprA wascarried out as described earlier [7]. The ESI-MS and SDS ⁄ PAGE of the purified FprA showed that the prepar-ation...
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Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt

Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt

... phosphorylatethree translation factors and two enzymes involved in the regulation of glycogen and trehalose synthesis,thereby coordinately controlling translation and sugarflux [8]. Further experiments ... against S6 and the GST-tag. (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 inPaskin+ ⁄ + and Paskin) ⁄ )MEFs. (D) Immu-noblot analysis of the phosphorylation status of p70S6K ... that the eukaryotic elongationfactor 1A1 (eEF1A1) is phosphorylated by PASKINat T432 [18]. However, the role of this modification intranslational control awaits further investigation. Inthe...
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Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

Báo cáo khóa học: O-acetylation and de-O-acetylation of sialic acids in human colorectal carcinoma docx

... suggesting that the level of O-acetylation is as high as80% in normal colonic tissue [3]. On the other hand, areduction in the level of sialate O-acetylation in colon cancerhas been demonstrated ... alteration related toprogression of colorectal cancer [8]. These observations,naturally, raise many questions concerning the occurrence and enzymatic processes involved in the O-acetylation and de-O-acetylation ... acids.Sialic acids consist of a family of acidic nine-carbon sugarsthat are typically located at the terminal positions on avariety of glycoconjugates. The largest structural variations of naturally...
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Báo cáo khoa học: Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase/helicase activities of hepatitis C and related viruses ppt

Báo cáo khoa học: Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase/helicase activities of hepatitis C and related viruses ppt

... shown).Attention was then directed to the effects of the variousanalogues on the ATPase activity of the HCV enzyme,monitored by release of 33Pfrom[c-33P]ATP. At an ATPconcentration corresponding ... and, given the bulkiness of the bromine atoms, these are primarilyresponsible for the hydrophobic interactions with the apolarchains of CK2. The only polar interaction, mediated by apair of ... [20] strong substrate/product inhi-bition of the unwinding reaction by the HCV enzyme wasobserved when the DNA substrate concentration exceeded15 pM(not shown). The responses of the helicase...
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Báo cáo khoa học:

Báo cáo khoa học: "A Comparison and Semi-Quantitative Analysis of Words and Character-Bigrams as Features in Chinese Text Categorization" potx

... than the curves for the CE collection because of sparseness; The CE collection is more sensitive to the additions of terms that come with the increase of dimensionality. The CE curves in the ... close to the word itself. For instance, “作曲” and “曲家” are sub-bigrams of the word “作曲家(music composer)”, both the bigrams and the word are on the top of the lists. Theretofore, the bigram ... orthogonal. This assump-tion might not affect the effectiveness of the models, but the semantic redundancy and com-plementation among the feature terms do impact on the classification efficiency...
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Báo cáo khoa học: Molecular organization and force-generating mechanism of dynein pot

Báo cáo khoa học: Molecular organization and force-generating mechanism of dynein pot

... clarify the mechanism of the coordination, modulating the taildomain could be one of the mechanisms of regulation of dynein motility [94].In addition, dynein shows large variation in the mode of ... model The observations described above pose the model of dynein force generation, which is the most widely citedone: on binding of ATP to AAA1, the orientation of the docked linker on the head ... toidentify the optimal alignment between the hydropho-bic heptad repeats in the two strands of the coiled-coilstalk. Alterations in the phase of the heptad repeats in the CC1 changed the affinity of the...
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