Báo cáo khoa học: Modular metabolic control analysis of large responses The general case for two modules and one linking intermediate docx
Báo cáo khoa học: Modular metabolic control analysis of large responses The general case for two modules and one linking intermediate docx
... 2006 The Authors Journal compilation ª 2006 FEBS 201L. Acerenza and F. Ortega Metabolic control analysis of large responses Modular metabolic control analysis of large responses The general case ... measure the changes in the intermediate and the demand rate and, second, to perturb the demand module and measure the changes in the intermediate and the supply rate.This is the way that the experiments ... test the validity of the novel relationshipsderived here. The control analysis for large changes presented inthis work is, in our view, a completely general analysis for the case of two modules...
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Báo cáo khoa học: Modular metabolic control analysis of large responses in branched systems – application to aspartate metabolism potx
... used.Here, the general theory of modular MCA for large responses is developed to include the analysis of branched systems. This formalism enables the analysis of systems with three modules and one explicit intermediate. ... Ortega F (2007) Modular metabolic con-trol analysis of large responses. The general case for two modules and one linking intermediate. FEBS J 274,188–201.22 Saltelli A, Ratto M, Andres T, Campolongo ... on the flux control pattern for large responses. Fig. S3. Contributions of the inhibitions of the AK and HSDH activities (of bifunctional AK-HSDH) byThr to the flux control pattern for large responses. Doc....
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... foundbetween the site of the no. tag D tag and a poly(dA)among the cDNA of the gene (Hs. 436980). The blastresults of another 11 sequences in the GenBankTable 1. Overview of all tags analyzed with the ... product of no. 10.We further optimized the PCR annealing tempera-ture, as well as the cycle number, for each of the weak-band tags. Moreover, these bands were obviouslyclearer than the pervious ones ... 5¢-end and 3¢-end of all cDNAs,respectively, and after 25 cycles, the entire cDNAswere largely amplified for the next experiment.Figure 2 shows the amplified cDNAs. As can beseen, the length of the...
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Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf
... manner, the actual composition of the spots depends on the com-position of the lysates, the lysis buffer and the ‘affinity’ of the individual proteins for the solid phase. Varia-tions in lysis and ... correlationbetween the activation state, on the one hand, and the biological or disease state, on the other hand.Small molecules that modulate the activity of signal-ing proteins are useful tools for dissecting ... for the two most relevant parametersthat determine the potential of a compound as a drug,namely (a) the on-target activity as the primary mea-sure for pharmacological efficacy and (b) the off-targeteffects,...
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Báo cáo khoa học: "On-Line Semantic Analysis of English Texts" ppt
... (3) their cores are such that they are identical, or either is a member of the other in the sense of a list member, or the left- or right-hand member of either core is a member of the other. ... templates The fourth formula is the proper qualifier for the first, and, if such had been found for the second and third, they would have appeared in place of the NILs in the fifth and sixth ... by any method, then an ambiguity resolution for the words of the text can be read from it, and the cor- rectness or otherwise of the resolution is some test of the adequacy of the original representation....
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Báo cáo khoa học: Sulfoquinovosylmonoacylglycerol inhibitory mode analysis of rat DNA polymerase b pdf
... interfaces of the SQG and fatty acid separately. We then combinedthese two interaction interfaces and identified the SQMG interaction interface of the 8-kDa domain. Analysis of the SQG interface with the ... amount of SQG or MA (i.e. I ⁄ P ¼ 10)did not inhibit the ssDNA binding activity of the 8-kDa domain of pol b (Lanes 3 and 4 of Fig. 3C). On the other hand, a mixture of SQG and MA inhibited the ... ratio of 1, and it disappearedat the ratio of 0.1, suggesting that one molecule of SQMG(C14:1) competed with one molecule of M13ssDNA and subsequently interfered with the binding of DNA to the...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
... to the scheme suggested by Duggleby [13,14]in which E ¢ indicates the inactive form of the enzyme, and J1, J2 and J3are the rate constants for inactivation of the respective forms of the ... in the course of the continuouslymonitored reactions were analysed as described in the Materials and methods. For the reactions in the absence of fatty acids, or in the presence of oleate and arachidonate, ... kp¼k2:k3k2þk3 and the equilibrium associa-tion constant for the product Ki¼kÀ3k3. Although the Km and the kpderived for Model I and Model II have differentalgebraic form, their meaning within the...
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Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt
... seen as band doubling for T)11 and T+2. Therefore, the measured contribution of theseforms to the integrated intensity of these two bases wasused in the calculation of the contribution of individualbases ... factor,oxki,Mg⁄oxki,with the largest values of 4.4 for T+1 and 3.5 for T+2, and much smaller, in the range 1.6–2.3, for the most reactive groups including T)3, T)4, T)8 and T)11. For the least reactive, T)9, the ... of oxEa for the oxidation of T+1 and T+2, which are located close to the activecenter of RPo, correlate with the lower reactivity of these bases. For the reactions carried out in the pre-sence of...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... essential for eukaryotic DNAreplication and are the most likely candidates for the replicative DNA helicase responsible for unwindingDNA at the replication forks [1–3]. Consistent withtheir primary ... X-100 used for cell fractionation and then incubated at 37 °C for 15 min in the same buffer before fixation (Figs 9 and 10).Cells were washed with NaCl ⁄ Pi and then permeabilized and blocked ... identicalcell for the experiments in Fig. 5. Cells were selected underphase-contrast illumination, and each was brought to the center of the field of the microfluorometer. First, the intensity of DAPI...
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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... separately for the numerator and the denominator of Eqn (5). Basedon the SD of the numerator and the denominator the SEM of qhwas computed, assuming statistical independence of the two. The time-dependent ... contained5 lL of SYBR Green PCR Core Kit (Bioke, Leiden, The Netherlands), 3 pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and 3 lL of cDNA template ... starvation. Data were normalizedto the mean of the control gene PDI1 and the steady-state samples of the nitrogen starvation experiments. The error bars represent the SEM of three independent experiments...
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