Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

... properties of PLC-d1 and the PLC isoforms at the membrane-associated state through the PS-dependent structural and func- tional alterations of the PH domains. Although the a2-helix located in the b5 ... compilation ª 2006 FEBS 187 Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology dom...

Ngày tải lên: 30/03/2014, 10:20

... in the Q 0 band [25,26]. The splitting of these bands has been regarded as a lowering of the heme symmetry in HbA-CO [17,27], due to nonperpendicular iron–ligand bond above the xy plane of the ... the interactions between the heme and the surrounding globin, being influenced by the attached ligand and thereby by the spin state of the iron atom [11]. According...

Ngày tải lên: 23/03/2014, 12:20

... binding of the methionine sulfoxide residue at the S 1 ¢ subsite of the active centers of NE and Pr3. The fact that the S 1 ¢ subsite of Pr3 is significantly smaller than that of NE [21] might then explain ... oxida- tion of M51 and M89 of M25L elafin and M63L trap- pin, respectively. On the other hand, oxidation of M25L elafin and M63L trappin did not signific...

Ngày tải lên: 19/02/2014, 07:20

... catalysis. The isoalloxazine ring system is located at the ‘base’ of the pocket. The active site is on the re face of the isoalloxazine for the noncovalent form of the enzyme and on the si face for the ... about the mode of access of dioxygen to the active center, the locus of the O 2 interaction with the reduced flavin, or – in the cases of flavo...

Ngày tải lên: 07/03/2014, 00:20

... composed of an a -domain and a b -domain, both of which are common in the previ- ously reported structures for other c-type lysozymes. The a -domain is composed of four a-helices (A–D), and the b -domain ... residue exists in the b -domain, and is oriented to the interface between the two domains. Therefore, the interface of BSL2 is less susceptible to unfolding...

Ngày tải lên: 23/03/2014, 04:21

... of south-east Asia, parts of Africa, and Southern and Central America. The Aedes aegypti mosquito, which prefers to feed on humans, is a carrier of the dengue virus and is commonly found on the ... to hybridize with the probe sequences. Figure 5 shows the overlapping expression patterns for two pairs of genomes for the set of 216 probes. The distribution of th...

Ngày tải lên: 23/03/2014, 11:20

... characterization of their expression using Northern analysis and in situ hybridiza- tion. The pattern of DAT RNA expression in the caterpillar CNS was shown to differ from that of OAT and thus, unlike the ... sequence of 3.8 kb was assembled by joining the two RACE sequences at a region of overlap in the center of the original 612 bp sequence. Two primers were then...

Ngày tải lên: 23/03/2014, 20:22

... day 9, and 9.5-fold on day 12. The level was retained to day 15. Similarly, the band shift efficiency in the H 2 O 2 -cultures increased by 6.7-fold of the control in 15 days and reached the plateau ... plateau of 10.7-fold activation on day 20 (Fig. 4A). Presence of the NF-jB p65 protein in the shifted complex was confirmed by detection of a super-shift band using a...

Ngày tải lên: 24/03/2014, 04:21

... aspects of life. Some of these cofactors, such as vitamin B 12 and vitamin H (biotin), catalyze a small but nevertheless important set of biochemical reactions. Other cofactors, on the other hand, ... (RnfG and RnfD) via an ester linkage between the hydroxyl group of a threonine and the ribitylphosphate side chain of the cofactor was discovered [46]. On the other han...

Ngày tải lên: 28/03/2014, 22:20

... aspects of life. Some of these cofactors, such as vitamin B 12 and vitamin H (biotin), catalyze a small but nevertheless important set of biochemical reactions. Other cofactors, on the other hand, ... (RnfG and RnfD) via an ester linkage between the hydroxyl group of a threonine and the ribitylphosphate side chain of the cofactor was discovered [46]. On the other han...

Ngày tải lên: 28/03/2014, 22:21