Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptide

Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-afﬁnity thrombopoietin antagonists and agonists pot

... 95 Grafting of thrombopoietin- mimetic peptides into cystine knot miniproteins yields high-afﬁnity thrombopoietin antagonists and agonists Sebastian Krause 1, *, Hans-Ulrich Schmoldt 2, *, Alexander ... concept of grafting peptides with desired biological activities onto the structural scaffold of small disulﬁde-rich inhibitors with cystine knot (CK) f...

Ngày tải lên : 30/03/2014, 10:20

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Báo cáo khoa học: Study of synthetic peptides derived from the PKI55 ppt

... peptides. The level of PKC a, b 1 , b 2 and f isoforms was also studied. Results Synthesis of peptides derived from PKI55 and their inhibitory effect on rat brain PKC A series of peptides was synthesized ... the enzyme activity of a, b 1 , b 2 , c, d and f, but not of e PKC isoforms (Fig. 1). Peptides 5, 8 and 9 were tested in vitro at a concentration of 6 lm on the...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... mode of entry of HGV ⁄ GBV-C into target cells is not known. Elucidation of the mechanism of the fusion of envel- oped viruses with target membranes has attracted considerable attention because of ... Turbidity (A 436 ) of dispersion of egg PtdCho ⁄ brain PtdSer SUVs in the absence (solid line) and presence of the E2(7–26) (black) and W-E2(279–298) (grey) peptides at...

Ngày tải lên : 07/03/2014, 17:20

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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf

... The speciﬁc activities of the 5·-RNase HI and 6·-RNase HI proteins were 65% and 43%, respectively, of that of the wild-type protein, and 93% and 66%, respectively, of that of the 4·-RNase HI protein (Table ... entire structure (A), N and C represent the N- and C-termini of the protein, and a and b represent the a helix and b strand, respectively. The side-chain...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt

... 1, 2.5 lg of GST-ExoS(wt); lane 2, 1.25 lg of GST-ExoS(wt); lane 3, 0.75 lg of GST-ExoS(wt); lane 4, 2.5 lg of GST-ExoS(L426A); lane 5, 1.25 lg of GST-ExoS(L426A); lane 6, 0.75 lg of GST-ExoS(L426A); ... 2.5 lg of GST-ExoS(D427A); lane 8, 1.25 lg of GST-ExoS(D427A); lane 9, 0.75 lg of GST-ExoS(D427A); lane 10, 2.5 lg of GST-ExoS(L428A); lane 11, 1.25 lg of GST-ExoS(L428A...

Ngày tải lên : 19/02/2014, 08:20

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Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... nucleotides 262– 237 of the reverse strand of the b3Gal-T5 coding sequence; lower strand primer, 5¢-CAGGTTCAGGGGGAGGTGT GGGAG-3¢, corresponding to nucleotides 31–8 of the reverse strand of the SV40 polyadenylation ... oligosaccharides formed in BxPC3 cells (j in lower part, and A, B, and C of upper part) andT5ASclone(h in lower part, and A, B, and C of upper part) metaboli...

Ngày tải lên : 19/02/2014, 12:20

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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... the C-terminus of TnrA, and may play a role in the regulation of TnrA activity and its proteo- lysis [15]. To test this assumption, various truncations of TnrA (lacking six, 20 and 35 amino acids ... proteins, puriﬁcation of GlnK-ST and GS-ST, and glutaralde- hyde crosslinking assays. This supplementary material can be found in the online version of this article. Please no...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... the synthesis and insertion of the metal centre [6,7]. Two of the products of the hyp genes, namely HypA and HypB, are involved in nickel insertion [8,9]. From the other four hyp gene products, HypF and ... analysis of mutant variants of HypE demonstrates that the carb- amoylation of th e C-terminal thiol and the d ehydration of the S-carboxamide to the thiocyanate are i...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

... C. Aalberse and Ronald van Ree CLB Department of Immunopathology and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, the Netherlands The mature ... (Fig. 6A). A band with s imilar molec- ular mass to that of the prosequence appeared with increasing intensity on addition of increasing doses of nDer p 1. Surprisingly, no cle...

Ngày tải lên : 17/03/2014, 11:20

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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

... alone and in complex with RNA, and to prepare Fig. 9. Determination of the RNA content of RNA–UDE by real- time RT-PCR. The RNA content of RNA–UDE was isolated and reverse-transcribed, using a random ... process nucleic acids are often capable of binding to both RNA and single- stranded or double-stranded DNA, producing altered biological functions, depending on the type of...

Ngày tải lên : 22/03/2014, 16:21

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