Báo cáo khoa học: Physico-chemical properties of molten dimer ascorbate oxidase doc

... 70–81. Probing ascorbate oxidase unfolding routes E. Nicolai et al . 5204 FEBS Journal 273 (2006) 5194–5204 ª 2006 The Authors Journal compilation ª 2006 FEBS Physico-chemical properties of molten dimer ascorbate oxidase Eleonora ... therefore this dimeric intermediate has been defined as a molten dimer (MD) state [15]. The structural properties of this partially unfo...

Ngày tải lên: 30/03/2014, 10:20

... DNA as a homodimer. The CTDs of k CI [1] and LexA [18] (i.e. the struc- tural homologs of /11 CTD) are involved in the homodimerization of these repressors. Sequence align- ment of /11 CI and ... involved in the dimerization of LexA CTD were also present in the CTD of /11 CI (data not shown). The CTDs of two /11 CI monomers may therefore be responsible for the formation of a d...

Ngày tải lên: 07/03/2014, 00:20

... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in prox- imity of the N-terminus of the b 3 ‘open’ TM helix. Overall evaluation of the ... (involving the swing-out of the b 3 hybrid domain [6]) taking place without the requirement of full separation of the TM helices. Since then, crystallographic structures of the...

Ngày tải lên: 18/02/2014, 04:20

... of cytoplasmic composition of anionic li- pids in membrane binding of K-Ras was evaluated by studying the effect of ATP depletion, which inhibits inward movement of PS, with consequent loss of plasma ... the role of electrical properties of the plasma membrane in the subcellular distribution of K-Ras. In particular, we investigated (a) the role of surface charge on inner...

Ngày tải lên: 19/02/2014, 00:20

... (uninhibited enzyme) to 100% (the lowest activity of the enzyme). Dimerization of cysta- tin F leads to a loss of inhibitory activity. (C) Immunoblot of cystatin F run on nondenaturing PAGE. Cystatin ... suggest that dimerization of cystatin F is linked to disulfide bond formation, which is responsible for the loss of inhibitory activity of the pro- tein. We also noticed that mon...

Ngày tải lên: 19/02/2014, 17:20

... thank Prof. Steve C. Fry, Institute of Cell and Molecular Biology, Univer- sity of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, Ruhr University of Bochum, Germany, for critical reading of the ... quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 33 Laemmli UK (1970) Cleavage of structural proteins dur- ing the assembly of the...

Ngày tải lên: 20/02/2014, 01:20

... one of a few major Myb-interacting proteins. The moderate basal level of sumoylation was greatly enhanced by cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing ... to the importance of the c-Myb–Ubc9 interaction. We conclude that SUMO-1 conjugation of c-Myb is not only a phenomenon induced under favourable conditions of overexpression of...

Ngày tải lên: 21/02/2014, 00:20

... amongst arthropod phenoloxidases, but has not been reported previously simply because of a lack of studies. The inhibition of activated hemocyanin by typical inhibi- tors of phenoloxidase was similar ... [10,11]. The production of o-quinones by phenoloxidase is the first step in mela- nin biosynthesis [7,12,13]. The structure of phenol- oxidases involves two different types of mo...

Ngày tải lên: 07/03/2014, 05:20

... level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data summar- ized in Table 1, it can be seen that some of the ... of the monomeric form of SV-IV is approximately doubled by the addition of 5.4 m M SDS (Fig. 1), whereas in the 1–70 region, the a-helix content increases from 2–3% to  23% in the p...

Ngày tải lên: 07/03/2014, 14:20

... role of Hsp20. However, investigation of the biochemical properties of isolated Hsp20 lag behind. Indeed, the biochemical properties of rat Hsp20 were only briefly characterized in the reports of ... summa- tion of the elution profiles of the isolated 3D mutant of Hsp27 and isolated wild-type Hsp20 is presented on curve 1. Experimental elution profiles of the mixture of t...

Ngày tải lên: 07/03/2014, 15:20