Báo cáo khoa học: A family of killer toxins Exploring the mechanism of ADP-ribosylating toxins pptx

... residues, vari- able oxidation of methionine and variable deamidation of asparagine and glutamine. Parent and fragment mass toler- ances were set to 1 Da. Up to two missed cleavages and half tryptic ... JD, Amanchy R, Kristiansen TZ, Jonnalagadda CK, Surendranath V, Niranjan V, Muthusamy B, Gandhi TK, Gronborg M et al. (2003) Development of human protein reference database as an initial...

Ngày tải lên: 07/03/2014, 06:20

... light adaptation of the eye. Light adaptation also induced the decrease of all-trans isomer both in the distal and proximal portions of the retina. In contrast, the increase of 11-cis ligand was ... using an ATTO AE6905C Image Saver, and quantified with NIH IMAGE program. The gel was then stained with Coomassie Brilliant Blue, and the protein content was measured via the...

Ngày tải lên: 17/03/2014, 03:20

... ethanol, sonicated and extracted overnight at +4 °C [23]. After centri- fugation, the pellet was discarded and a sample of the supernatant was withdrawn for radioimmunoassay of insulin and glucagon ... Both end-plate potentials of the ion trap were set at 1.5 V and the duration of the electron pulse was 100 ms. Data acquisition and handling Primary data analysis was perfor...

Ngày tải lên: 17/03/2014, 10:20

... activator of transcription 5A Yoshihisa Watanabe 1 , Masaya Ikegawa 2 , Yoshihisa Naruse 3 and Masaki Tanaka 1 1 Department of Cell Biology, Research Institute for Neurological Diseases and Geriatrics, ... suppresses the tran- scriptional activity of STAT 5A. Discussion We isolated a novel STAT 5A splicing variant from the mouse brainstem. The STAT 5A_ DE18 variant lacked th...

Ngày tải lên: 23/03/2014, 04:20

... given the more recent studies, a re-examination of the role of Ras in the development of these cells seems to be war- ranted. With regards to transcriptional targets of the Itk pathway, although the ... factors nuclear factor for activated T cells (NFAT), nuclear factor kappa-light chain enhancer of activated B cells (NFjB) and activator protein-1 [4]. A number of st...

Ngày tải lên: 28/03/2014, 22:21

... a manner similar to that of the catalytic glutamate of the ADPRT and this could be essential to the mech- anism and form part of the mechanism that has not yet been captured in a crystallographic ... M, Murata T, Nakayama K, Asakawa K, Ohara M, Komatsuzawa H & Sugai M (2001) Complete nucleotide sequence of a Staphylococcus aureus exfoliative toxin B plasmid and ide...

Ngày tải lên: 30/03/2014, 10:20

... or SYK themselves, is capable of transforming NIH-3T3 cells [70]. In addition, we and others have demonstrated that the activation and plasma membrane localization of the fusion construct are dependent ... F, Cazzola G, Consolini R, De Mattia D, Dell’Erba G et al. (2002) Clinical, immunological, and molecular analysis in a large cohort of patients with X-linked agammaglobulinemia:...

Ngày tải lên: 14/02/2014, 18:20

... 5¢-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab- start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3¢;Abstop, ... 5¢-CCTGCCGAGCTCCTATTA CACAACGCCACCAACCATCAG-3¢. The PCR solution was prepared in the buffer supplied with the enzyme, and contained Aba, Abb and Abcat 40 nm each, and the start and stop...

Ngày tải lên: 18/02/2014, 13:20

... the performance of the potential will only increase with the size of the structural database and as terms are added to the model to account for protein and RNA intra-molecular interactions that are ... Given the relative sparsity of the structural database, we investigated whether a reduced-atom representation would not lead to improved performance in the protein–RNA c...

Ngày tải lên: 18/02/2014, 16:20

... [46]. The appear- ance of the LIRs probably provides humans with an evolutionary advantage and contributes to the specia- tion of primates. Experimental procedures Identification of LIRs The human ... termed arms) that are approximately complemen- tary to each other. Generally, there is a spacer between the arms, and the full structure of an inverted repeat can form a s...

Ngày tải lên: 07/03/2014, 00:20