Báo cáo khoa học: Solution structure of an M-1 conotoxin with a novel disulfide linkage pdf

... the dyana algorithm. The outcome was a set of 20 structures with a mean global rmsd of 0.56 ± 0.16 A ˚ and a mean global heavy atom rmsd of 1.30 ± 0.28 A ˚ . Structural refinement was carried ... Solution structure of an M-1 conotoxin with a novel disulfide linkage Wei-Hong Du 1,2, *, Yu-Hong Han 3,4, *, Fei-juan Huang 1, *, Juan Li 2 , Cheng-Wu Chi 3,4 a...

Ngày tải lên: 30/03/2014, 09:20

... ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio 1 , Chiara Gasparetti 2 , Martina Andberg 2 , Harry Boer 2 , Anu Koivula 2 , Kristiina Kruus 2 , Juha ... Paloheimo M, Valtakari L, Puranen T, Kruus K, Kallio J, Ma ¨ ntyla ¨ A, Fagerstro ¨ m R, Ojapalo P & Vehmaan- pera ¨ J (2006) Novel laccase enzyme and use thereof. Europ...

Ngày tải lên: 14/02/2014, 18:20

... have determined the structure of HtA by NMR methods. The structure consists of one a- helix, a helical turn and seven b-strands that form an N-terminal hairpin and an anti-parallel b-sheet, with ... were analyzed in a semi-automated iterative manner by cyana 2.1 [38]. The NOE coordinates and intensities used as input for automated analysis were generated auto- matically by...

Ngày tải lên: 18/02/2014, 08:20

... that protein family. In addition, we show that both its fold and potential surface partially resemble the structural features of the antimammalian scorpion a- neurotoxins and of the human antibacterial ... distance data by dynamical simulated annealing from a random array of atoms. Circumventing problems associated with folding. FEBS Lett. 239, 129–136. 31. Laskowski, R .A. , Rullm...

Ngày tải lên: 20/02/2014, 11:20

... duplex at molar ratios of 1 : 1.2 and of 1 : 2.4. Spectra analysis NMR data were analysed and processed with the computer programs XWINNMR , AURELIA [22], and AUREMOL [23] (Bruker, Karlsruhe, Germany). ... rmsd values given in Table 1. Analysis of the Ramachandran plot shows that the dihedral angles / and w for the secondary structure elements are all found in the most favored or th...

Ngày tải lên: 21/02/2014, 00:20

... physiologi- cal substrate remains a mystery [17]. Structurally, RICH protein consists of three regions: a glutamate- and aspartate-rich N-terminal domain, a catalytic phosphodiesterase domain, and a C-terminal isoprenylation ... catalytic domains of RICH and CNPase share a pair of conserved sequence motifs H-X-(T ⁄ S)-X (Fig. 1B) with three other groups of enzymes: fungal...

Ngày tải lên: 07/03/2014, 10:20

... chain protons of Leu86 and protons of amino acids Ala14, Val55 and Leu81 are observed, none of which are seen for the wild-type protein. Analysis of the backbone dihedral angles F and Y of mutant ... -type and mutan t form of HPr are visualized by a comparison of t he co rrespondin g backbo ne dihedral angles. Values for the wild-type and the mutant protein are indicated by w...

Ngày tải lên: 07/03/2014, 16:20

... Both mastoparan and D-somatostatin blocked refolding. Fig. 6. NMR titrations of 2 mM mastoparan by human PDI-bb¢ protein and a plot of the magnitude of changes in mastoparan proton chemical shifts ... NMR or X-ray crystallogra- phy. The overall shape of full-length human PDI has been investigated by small-angle X-ray scattering and shown, at low resolution, to adopt a flat annular...

Ngày tải lên: 16/03/2014, 04:20

... Watson and Crick b ase pair formation between antiparallel strands. The model iso ADNA duplex is characterized by the s ame value of slide, X-displacement and the helical parameters, as ADNA. On the ... Sundaralingam 3 and Narayanarao Yathindra 1 1 Department of Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India; 2 Department of Chemical Sciences, TIFR...

Ngày tải lên: 16/03/2014, 18:20

... violations greater than 0.5 A ˚ and r.m.s.d. for bond and angle deviations from ideality of less than 0.01 A ˚ and 5°, respec tively. Structure calculations for mouse aMT All structure calculations ... Solution structure of Cu 6 metallothionein from the fungus Neurospora crassa Paul A. Cobine 1 , Ryan T. McKay 2, *, Klaus Zangger 2, †, Charles T. Dameron 3 and Ian M. Armitage...

Ngày tải lên: 23/03/2014, 13:20