Báo cáo khoa học: Perturbation of membrane microdomains in GLC4 multidrug-resistant lung cancer cells ) modification of ABCC1 (MRP1) localization and functionality docx

... (200 7) 1470–1480 ª 2007 The Authors Journal compilation ª 2007 FEBS Perturbation of membrane microdomains in GLC4 multidrug-resistant lung cancer cells ) modification of ABCC1 (MRP 1) localization and ... were obtained. MRP1, GM1 and caveolin-1 distribution within membrane fractions In order to determine the effect of MbCD on the integrity of...

Ngày tải lên: 30/03/2014, 09:20

... [22], we linearize the system of Eqn ( 3) in the vicinity of the steady state. We do this to investigate the stability of this state. In this way we obtain the in uence a small change in the concentration ... cycles in such a combination do not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the mag...

Ngày tải lên: 19/02/2014, 16:20

... interaction of the calcium binding troponin (troponin C) with bivalent cations and the inhibitory protein (troponin I). Biochem. J. 137, 145–154. 23. Hartree, E.F. (197 2) Determination of protein: a modification ... R.S. (199 7) Mapping of a second actin-tropomyosin and a second troponin C binding sites within the C terminus of troponin I, and their importance in the C...

Ngày tải lên: 20/02/2014, 11:20

... 323 58.7 ± 1.1 ()7 .6 %) 59.5 ± 1.1 ()6 .3 %) E2 56.0 ± 0.8 ()5 .6 %) 55.4 ± 1.3 ()6 .6 %) P1 NA 62.5 ± 1.8 ()1 .6 %) 60.7 ± 0.9 ()4 .4 %) P2 57.1 ± 1.1 ()3 .7 %) 55.7 ± 0.8 ()6 .0 %) In the case of the OS ⁄ NS ... ()3 .2 %) B1 K28 300 46.8 ± 0.6 ()2 0 %) 55.2 ± 0.5 ()6 .0 %) B2 323 55.9 ± 1.3 ()1 2 %) 61.6 ± 0.8 ()3 .0 %) B...

Ngày tải lên: 18/02/2014, 08:20

... equivalent weight of PE, PS, PtdIns, PG (Sigma), PtdIns(3)P (Cay- man, Ann Arbor, MI), PtdIns(4)P (Cayman), PtdIns(5)P (Cayman), PtdIns(3,4)P 2 (Cayman), PtdIns(3,5)P 2 (Sigma), PtdIns(4,5)P 2 (Cayman), and ... liposomes of PS ⁄ PC (black), PtdIns(4,5)P 2 ⁄ PC (green), PtdIns(3,4,5)P 3 ⁄ PC (brown), PG ⁄ PC (lime), PtdIns(3,5)P 2 ⁄ PC (cyan), PtdIns(3)P ⁄ PC (yellow), PtdIns(4)P ⁄ PC (b...

Ngày tải lên: 19/02/2014, 05:20

... activity of the RPTPa D2 domain is required for Src binding and activation It is well known that the membrane distal domains of RPTPs are involved in protein–protein interactions [16–18,20,28]. We investigated ... specificity of D1 in vitro [10,11]. In addi- tion, the D2 domains were shown to be involved in intermolecular and intramolecular interactions, as well as in hom...

Ngày tải lên: 15/03/2014, 10:20

... ± 1.5 pmol min )1 Æmg )1 of mitochondrial protein (n ¼ 5); after the addition of 5 mm glutamate and 5mm malate, 88.2 ± 5.7 pmol min )1 Æmg )1 of mitoch- ondrial protein (n ¼ 5); and after subsequent ... nmolÆmg )1 of mitochondrial protein. The combination of 3.5 lm Ca 2+ and H ⁄ R led to the substantial decrease in GSH of 1.27 ± 0.15 nmolÆmg )1 of...

Ngày tải lên: 16/03/2014, 22:20

... partitioning of Pgp into intermediate- density microdomains. A B Fig. 1. Sucrose density gradient partitioning of P-glycoprotein (Pgp) and markers of clas- sical lipid rafts. (A) CH R B30 cells ... colocaliza- tion of the two proteins by confocal fluorescence microscopy. We first examined the colocalization of GM 1 and caveolin-1 in CH R B30 cells. The individual staining p...

Ngày tải lên: 16/03/2014, 23:20

... of proline (< 1 M ), enhanced the rate and extent of reactivation of creatine kinase. In the limited instances in which wehave examined the effect of low proline concentrations (< 1 M ) ... m M dithiothreitol, pH 7. 6), or in buffer containing 3.4 M proline (B) or in buffer con- taining 1.6 M TMAO (C). Each line represents a single experiment. Fig. 6. Effect of...

Ngày tải lên: 23/03/2014, 15:21

... on P. aeruginosa [16–18]. These efflux pumps of P. aeruginosa consist of two inner membrane- associated components, MexB (MexD, MexF, and MexY) and MexA (MexC, MexE, and MexX), and an outer membrane ... we examined the accessibility of a-chymotrypsin to tyrosine 185 and 196 of OprM existing in the cell. Hence, P. aeruginosa cells were suspended in NaCl/ P i and dig...

Ngày tải lên: 23/03/2014, 21:21