Báo cáo khoa học: Slow conformational dynamics of the guanine nucleotide-binding protein Ras complexed with the GTP analogue GTPcS pot

... between the corresponding T 2 relaxation times for the conformational states 1a and 1b of Ras( T35S)•Mg 2+ GTPcS. Complex of Ras Mg 2+ GTPcS with the Ras- binding domain of Raf-kinase Addition of the ... al. Conformational dynamics of Ras bound to GTPcS FEBS Journal 274 (2007) 1419–1433 ª 2007 The Authors Journal compilation ª 2007 FEBS 1421 Conformatio...

Ngày tải lên: 30/03/2014, 09:20

... A ˚ 2 higher than the average B factor of the subunit. In the subunits with bound NAD + , the B factor of the two residues is comparable with the average B value of the subunit. Nevertheless, the conformation ... [9]. With the exception of Gln193, these residues are located in the deep active-site cleft. The exact boundaries of the flexible loop differ a...

Ngày tải lên: 18/02/2014, 16:20

... treatment of the collected pools to determine the distribution of sialic acid. Next, we completed online MS ⁄ MS analysis of the major species; some of these species were further analyzed by TOF ⁄ TOF ... cRBCs. Defining the glycans present on the surface of cRBCs will allow either for the design of strategies to optimize the agglutination assay or the design of a...

Ngày tải lên: 14/02/2014, 19:20

... implications of the observed VpUcyt structure One of the most studied activities of VpU is the induc- tion of CD4 degradation in the endoplasmatic reticu- lum of infected CD4 + cells. Only the cytoplasmic domain ... calculation of preliminary protein structures [79]. radar runs in an iterative manner. The input of the first cycle consisted of the 3D NOESY data...

Ngày tải lên: 18/02/2014, 06:20

... considering the oxidation of the substrate by T. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida- tion of the substrate. For most of the substrates ... the C-termi- nus of the protein, the most C-terminal tryptic peptide, as compared with the theoretical tryptic pep- tide map of the deduced prote...

Ngày tải lên: 19/02/2014, 06:20

... Equal mobilities of the radioactive and the protein- containing bands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical cross-linking reaction was calculated as the ratio of the ... introduction of the TFMDPh group in close proximity to the oxoG residue has no effect on the recognition and binding of DNA duplexes by the Fpg...

Ngày tải lên: 20/02/2014, 11:20

... bond between the hydrogen of OH9 (H O 9) and the oxygen of OH7 (O H 7) which can contribute to the stabilization of the C7–C9 region of the macrolide. Structural analysis of nystatin A1 The spectral ... along the long axis of the polyene with the D -mycosamine front (indicated with an arrow). The bonds of the hydroxyl groups are represented with bold...

Ngày tải lên: 21/02/2014, 03:20

... shows the distribution of fapb6 transcripts in the distal region of the intestine (A), liver (B), ovary (C) and the adrenal homo- log of the fish kidney (D). Panel 2 shows the locations of the distal region ... localize the rat Fabp6 protein and in situ hybridization to localize Fabp6 transcripts to the enterocytes of the ileum, luteal cells of the ovary and...

Ngày tải lên: 07/03/2014, 06:20

... inde- pendent of the origin of the cloned cDNA and whether the enzymes are naturally located in mitochondria. However, the data do not demonstrate the proper tar- geting of the proteins to mitochondria. Therefore, ultrastructural ... assembly of the iron– sulfur units. It is of interest therefore to know whether the fold- ing of these two proteins around their iron...

Ngày tải lên: 07/03/2014, 10:20

... at the acidic C-terminus of the protein is not occupied. MS of the nonreduced protein showed a mass of 23 700.0 Da. The difference between the molecular masses of the reduced and nonreduced protein ... analysis of the noncross- linked spinalin of the first peak, only monomers are present (Fig. 4A). The size of the particles is in agree- ment with this...

Ngày tải lên: 07/03/2014, 12:20