Báo cáo khoa học: Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton reaction potx

... FMN and highly catalyzed nitroreductase, flavin reductase and ferric reductase activities. This is the first demonstration of nitroreductase activity of DrgA protein previously identified by a drgA mutant ... Agriculture Journal compilation ª 2007 FEBS 1327 Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable o...

Ngày tải lên: 30/03/2014, 09:20

... after the AG1478 addition and analyzed for caspase 3 activ- ity by using a fluorometric substrate-based assay. Each point is the mean of the triplicate samples, and the bar represents the standard deviation. ... JNK is a mediator of the apoptotic action of AG1478. JNK activity in cells is tightly controlled by both protein kinases such as MKK4 or MKK7 and pro...

Ngày tải lên: 18/02/2014, 13:20

... s )1 in the presence of a stoichiometric amount of 2¢,5¢-ADP, and this is accompanied by an increase in the amplitude of the absorbance change. This is consistent with the notion that the 2¢,5¢-ADP ... binding to the reductase domain is thought to induce significant changes in the reduction potentials of the FAD[34].WeconcludethatinthecaseofCPRthe observed eff...

Ngày tải lên: 23/03/2014, 17:22

... double strand and the annealing of the new duplex finally results in either the replacement of the original strand or the expulsion of the invading strand, according to the kinetics and thermodynamic situation. ... digested by proteinase K [69]. In all, these results lead to the conclusion that the transient nature of the interaction between RNA and protein...

Ngày tải lên: 14/02/2014, 19:20

... con- centrations of these proteins are compared. Membrane binding on- and off-rates are probably important specifications for the functions of protein C ⁄ APC as well as the other Gla proteins. Association with ... capacity is a measure of the Gla protein packing density to sites provided by the mem- brane at saturating protein concentrations. Of rele- vance for t...

Ngày tải lên: 18/02/2014, 06:20

... phosphorylation of this signal transducer. The main transducer of BMPs how- ever, Smad1, resided mostly in the cytoplasm and was not phosphorylated. As expected, addition of BMP to the medium of these ... BMPs and STAT3 has been identified in the case of differen- tiation of neural stem ⁄ progenitor cells (NSCs). Here, the neurogenesis versus astrocytogenesis decisi...

Ngày tải lên: 19/02/2014, 00:20

... angiogenesis and progression [1–3,7]. Both the cancer cells themselves and nontumor support cells, such as fibroblasts, are sources of VEGF [8]. In contrast, in the case of coronary artery disease, ... to hypoxia, but this natural response is not sufficient to prevent the further progression of heart disease [9–11]. It is therefore important to understand the mecha- nism...

Ngày tải lên: 19/02/2014, 12:20

... up 60–65% of the compositions of 5a and 5a-like protein and 55% of ApAFP. Of the other amino acids, Thr (13%) is the next most abundant, and there are very low amounts of aromatic and long- chain ... 103.6%). As the sample was warmed from 4 to 8 °C, there was a small decrease in the intensity of the spectrum; however, from 8to16°C there was a very large d...

Ngày tải lên: 20/02/2014, 01:20

... Akt family of kinases, as well as the cross-reg- ulation between them. l The specific role(s) of each one of the SRPK family members and the significance of the ‘spacer domain’ for the functional ... adding to the picture of SRPK function and importance. TcSRPK, the SR protein kinase of a protozoan, the parasite Trypanosoma cruzi, which displays trans- a...

Ngày tải lên: 06/03/2014, 01:20

... trig- gers the ubiquitination and degradation of the protein [35]. It has, however, been suggested that the ability of SNP to both stimulate IRP2 degradation and induce ferritin synthesis is accomplished ... in the 5¢-UTR of ferritin mRNA and the 3¢-UTR of transferrin receptor (TfR) mRNA. Such binding blocks the translation of ferritin, the iron storage prot...

Ngày tải lên: 07/03/2014, 12:20