Báo cáo khoa học: Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton reaction potx

... FMN and highly catalyzed nitroreductase, ﬂavin reductase and ferric reductase activities. This is the ﬁrst demonstration of nitroreductase activity of DrgA protein previously identiﬁed by a drgA mutant ... Agriculture Journal compilation ª 2007 FEBS 1327 Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton reaction Kouji Takeda1, Mayumi Iizuka1, ... FAD and recombinant DrgA protein for ferric reductase activ-ity, and the same reaction mixture was used the addition of 200 lMH2O2for the Fenton reaction. The ﬁnal concentration of the...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... after the AG1478 addition and analyzed for caspase 3 activ-ity by using a ﬂuorometric substrate-based assay. Each point is the mean of the triplicate samples, and the bar represents the standarddeviation. ... JNK is a mediator of the apoptotic action of AG1478.JNK activity in cells is tightly controlled by both protein kinases such as MKK4 or MKK7 and protein phosphatases such as MKPs. MKP-1, the ... at the indicated time points after the AG1478 addition and analyzed forcaspase 3 activity by using a ﬂuorometric substrate-based assay.Each point is the mean of triplicate samples, and the...

Báo cáo khoa học: Interﬂavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx

... s)1in the presence of a stoichiometric amount of 2¢,5¢-ADP, and this is accompanied by an increase in the amplitude of the absorbance change. This is consistent with the notion that the 2¢,5¢-ADP ... binding to the reductase domain is thought toinduce signiﬁcant changes in the reduction potentials of the FAD[34].WeconcludethatinthecaseofCPRtheobserved effects of the binding of coenzyme and coenzymefragments ... and drugs [13–17].CPR is related to a number of mammalian diﬂavinreductases including the isoforms of nitric oxide synthase[18], methionine synthase reductase [19] and protein NR1Correspondence...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... double strand and the annealing of the new duplex ﬁnally results in either the replacement of the original strand or the expulsion of the invadingstrand, according to the kinetics and thermodynamicsituation. ... digested by proteinase K[69]. In all, these results lead to the conclusion that the transient nature of the interaction between RNA and protein is a prerequisite for the mode of action of (these) ... Stress-ing the notion of transient interactions between StpA and RNA even further is the fact that the protein is dispensable after the refolding of an RNA moleculehas occurred, and can be digested...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... con-centrations of these proteins are compared.Membrane binding on- and off-rates are probablyimportant speciﬁcations for the functions of protein C ⁄ APC as well as the other Gla proteins. Associationwith ... capacity is a measure of the Gla protein packing density to sites provided by the mem-brane at saturating protein concentrations. Of rele-vance for this discussion is the variation of membranebinding ... crystal structures of the Gla domain of prothrombin and factor VIIa, the placement of the seven Ca2+in relation to their Glaligands is almost identical in the two proteins [12]. The conformational...

Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx

... phosphorylation of thissignal transducer. The main transducer of BMPs how-ever, Smad1, resided mostly in the cytoplasm and wasnot phosphorylated. As expected, addition of BMP to the medium of these ... BMPs and STAT3 has been identiﬁed in the case of differen-tiation of neural stem ⁄ progenitor cells (NSCs). Here, the neurogenesis versus astrocytogenesis decisionduring early differentiation is ... identiﬁed as BMPs. The primary induction of PGCs is driven byBMP4 [44], whereas the number of PGCs is guided byBMP2, BMP4 and BMP8b in synergistic action [45]. As demonstrated by in vitro culture assays...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... angiogenesis and progression [1–3,7]. Both the cancer cells themselves and nontumor support cells, such as ﬁbroblasts, are sources of VEGF [8]. In contrast, in the case of coronary artery disease, ... to hypoxia, but this natural response is notsufﬁcient to prevent the further progression of heart disease[9–11]. It is therefore important to understand the mecha-nisms of VEGF regulation ... shown). As expected the CSD-containing cytoplasmic complexesbinding to the CSD site 1 (and CSD site 2) contained a protein of 50 kDa, consistent with the size of the CSD protein, dbpB (also known as...

Tài liệu Báo cáo khoa học: Hyperactive antifreeze protein in ﬂounder species The sole freeze protectant in American plaice docx

... up 60–65% of the compositions of 5a and 5a-like protein and 55% of ApAFP. Of the otheramino acids, Thr (13%) is the next most abundant, and there are very low amounts of aromatic and long-chain ... 103.6%). As the sample was warmed from 4 to 8 °C, there was a smalldecrease in the intensity of the spectrum; however, from8to16°C there was a very large decrease in the ampli-tude of the signal ... further change in the signal as the sample was warmed from 13 to 30 °C.To investigate whether the denaturation of thisextremely thermolabile protein is reversible, CD wasmeasured while the...

Báo cáo khoa học: Serine-arginine protein kinases: a small protein kinase family with a large cellular presence potx

... Akt family of kinases, as well as the cross-reg-ulation between them.l The speciﬁc role(s) of each one of the SRPK familymembers and the signiﬁcance of the ‘spacer domain’for the functional ... adding to the picture of SRPKfunction and importance. TcSRPK, the SR protein kinase of a protozoan, the parasite Trypanosoma cruzi,which displays trans- and cis-splicing and was cloned and characterized ... essentialfunction of the kinase in the germline development and embryogenesis of this organism [14]. The underly-ing mechanism for this function has not yet been eluci-dated, although the ﬁnding that...

Báo cáo khoa học: Iron regulatory protein-independent regulation of ferritin synthesis by nitrogen monoxide pot

... trig-gers the ubiquitination and degradation of the protein [35]. It has, however, been suggested that the ability of SNP to both stimulate IRP2 degradation and induceferritin synthesis is accomplished ... in the 5¢-UTR of ferritin mRNA and the 3¢-UTR of transferrin receptor (TfR) mRNA. Such binding blocks the translation of ferritin, the iron storage protein, and stabilizes TfR mRNA,whereas the ... It is likely that S-nitrosylation of a protein( s) involved in the activation of ferritintranslation is the mechanism underlying our ﬁndings,therefore further research is needed to delineate the players...

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