Báo cáo khoa học: The reconstitution of mammalian prion infectivity de novo pot

... biology of yeast and fungus supports this idea strongly, the direct proof of the prion hypothesis in mammals, specifically the reconstitution of the disease-associated isoform of the prion protein (PrP Sc ) ... biochemical nature of the prion infectious agent, and describes potential strategies and challenges rela- ted to the de novo generation of prion...

Ngày tải lên: 30/03/2014, 09:20

... net diffusion of K + from the cis chamber to the trans chamber. The reversal potentials of the two clearly identified conductance levels were indistinguishable, at )20.5 ± 1.5 and )20.6 ± 6.7 mV for the ... polymerization to F-actin on the trans side. None of the additions affected CLIC5 in the absence of actin, and neither G-actin nor F-actin mod- ified control bilayers...

Ngày tải lên: 30/03/2014, 04:20

... when-adverbials determine the position of the reference time independently from the speech time. Depending an which tense they are combined with they can either specify a reference time that precedes the ... how the calculus can be used for the interlingual analysis of the tense forms of verbs. I. The time axis model. The model is a temporal structure <time,&...

Ngày tải lên: 24/03/2014, 05:21

... as a nother identification of the complex at the position o f the histochemical stain in the left panel. Antisera against the SDHC subunit of complex II and against the Rieske protein of complex ... formation of supercomplexes and the effect of the absence of intact complex I, but the results are too preliminary in this regard. Heterologous expression of the E...

Ngày tải lên: 19/02/2014, 16:20

... nown of the mechanisms controlling the process of histone replacement. Two h ints from the literature could help to shed light on the underlying molecular events: one is the massive synthesis of ... that of chroma- tin condensation [59], reinforces the h ypothesis of a direct link between histone acetylation, th eir replacement and nucleus condensation. The m echanisms...

Ngày tải lên: 07/03/2014, 16:20

... 2¢-deoxy- ribonucleotides and are essential for de novo DNA biosynthesis and repair, regulating other enzymes in the DNA synthesis pathway via control of the nuc- leotide pool [1]. Of the four known classes of ... plot of L-ADP inhibition of dGTP-dependent D-ADP reductase in the presence of 2 mMD-ATP. (B) Secondary plot of the slope values of A. (C) Lineweaver- B...

Ngày tải lên: 07/03/2014, 17:20

... ‘intermediate’. The evolution of hemocyanin subunits follows the widely accepted phylogeny of the Hexapoda and provides strong evidence for the monophyly of the Polyneoptera (Plecoptera, Dermaptera, ... more widespread occurrence of hemocyanin in Hexapoda [14,22]. We decided to investigate a broad range of hexapod orders for the presence of hemocyanin mRNA (Table 1). T...

Ngày tải lên: 18/02/2014, 08:20

... that the effect of these additives on the chaperone action of aB-crys- tallin is dependent on the target protein used, and therefore the results highlight the need to assess the activity of chaperone ... further enhanced by it (Fig. 1C). The sig- nificant decrease in the amount of precipitation in the presence of high concentrations of Arg-HCl in the absence of...

Ngày tải lên: 18/02/2014, 16:20

... Indeed, there is evidence that the tails of these histones follow the path of the linker DNA [5]. They are crosslinked first to each other first and subse- quently to the core histones [19]. The ... proximities of the nucleosomes in the fibre. There is a considerable amount of mononucleosomes and much less di- ⁄ trinucleosomes in the products. The mononucleosomes eviden...

Ngày tải lên: 18/02/2014, 18:20

... accordance with this, the depend- ence of the fluorescence of the (+)-trans adduct on its position relative to the target dC was revealed in the case of the formation of the complexes of M.SssI with ... of the DNA double helix into the M.HhaI active-site pocket [45]. The flipped out cytosine forms contacts with the catalytic loop of the enzyme from the DN...

Ngày tải lên: 19/02/2014, 00:20