Báo cáo khoa học: Irreversible cross-linking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H2O2 ppt

... Irreversible cross-linking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H 2 O 2 Sakihito Kitajima 1 , Taise Shimaoka 2 , ... isoform is irreversibly cross-linked to a tryptophan residue facing the distal cavity. Mutation of this tryptophan to phenylalanine abolished the cross-linking...

Ngày tải lên: 30/03/2014, 08:20

... for the THF:A ligand. The presence of Mg 2+ had little effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making it comparable with binding to ... Thus, to address the in uence of the reaction conditions on the binding of Fpg and OGG1 to damaged DNA, we determined K d values for binding under the same condit...

Ngày tải lên: 18/02/2014, 18:20

... between the aromatic pyrimidine ring and the indole group of Trp27 should to be weaker in comparison with the purinetrione inhibitors due to the smaller size of the pyrimidine ring. It has in addition ... conditions, these apparent values can be used for comparison of binding affinities of the inhibitors under study. The fitting of the binding isotherms of al...

Ngày tải lên: 07/03/2014, 11:20

... treatment, (b) correlation of the interaction with phosphorylation of Ser5 of the C-terminal domain hepta- peptide repeat, and (c) the ability of C-terminal domain kinase inhibitors to abolish it. Polymerase ... as chromatin remodelling factors [2]. The carboxy-ter- minal domain (CTD) of RNA pol II has been implica- ted in mediating interactions with other factors involv...

Ngày tải lên: 07/03/2014, 11:20

... expressed in 293T cells became phosphorylated, possibly at its Akt phos- phorylation motif, in the absence of kinase inhibitors, whereas the phosphatidylinositol 3-kinase inhibitor wort- mannin and the ... DNA binding activity of dHAND could be affected by Akt phosphorylation. The in uence of phos- phorylation by Akt on the DNA binding activity was examined by gel s...

Ngày tải lên: 07/03/2014, 16:20

... adenine ring of AdoCbl on those of the enzyme-bound AdePeCbl is not possible without cleavage of the Co-C bond. The adenine ring of the coenzyme would be accommodated to the adenine-binding pocket ... binding to the enzyme in the pres- ence of ethanolamine, although they showed red or blue shift of the a-band by less than 6 nm (Fig. 3J–L). The steady-stat...

Ngày tải lên: 07/03/2014, 21:20

... in the hydrophobic interior of lipocalins – but rather serve for cross-linking the N- and C-terminus to the b-barrel [5] – they are not as crucial for folding as is the case for immunoglobulins. ... and digoxigenin are bound at the bottom of the cleft that harbours biliverdin IX c in the BBP [24]. Thus, while the overall topology of the lipocalin, comprising t...

Ngày tải lên: 23/03/2014, 07:20

... for the hydrolysis by TOP of the peptides containing Ile, Val and Asp deviated from the linearity above 25 °C. The plot for the reaction of the peptide containing Ala with TOP was not linear in ... doi:10.1046/j.1432-1033.2002.03129.x Leu-enkephalin from the enkephalin-containing peptides [22], and the specific TOP inhibition increased Met-enke- phalin antinociception...

Ngày tải lên: 23/03/2014, 21:21

... As the capability of binding of insulin and the 4-kDa peptide to the 43-kDa protein is similar, in our previous paper we named the 4-kDa peptide as leginsulin. But the 4-kDa peptide is not insulin, ... were prepared by the Escherichia coli protein expression system. The assay of binding activity of the mutant 4-kDa peptides to the 43-kDa protein was carried o...

Ngày tải lên: 31/03/2014, 07:20

... successful metabolic engineering of freezing tolerance in plants should include such an analysis of the dynamics of metabolism to gain com- prehensive information about the effects of individual overexpression ... are reversibly interconnected. The rate of sucrose export to sink organs (r Suc fi Sinks ) was calculated as the difference between the spline-interpo- late...

Ngày tải lên: 14/02/2014, 22:20