Báo cáo khoa học: Gene expression waves Cell cycle independent collective dynamics in cultured cells doc

... Gene expression waves Cell cycle independent collective dynamics in cultured cells Masa Tsuchyia 1 , Sum T. Wong 2 , Zhen X. Yeo 3 , Alfredo Colosimo 4 , Maria C. Palumbo 4 , Lorenzo Farina 5 , ... safely interpreted as demonstrating cell cycle independent collective dynamics. In this case too, we adopted a completely unsupervised approach by calculating the P...

Ngày tải lên: 30/03/2014, 08:20

... background staining (thin lines). Cells were then acquired in a FACScali- bur and analyzed using CELLQUEST. (A) Histograms show the expression of the molecules studied in HepG2 cells cultured in the ... (thin lines) or with rab- bit Igs (thick lines) as first step in permeabilized cells. (C) Histo- grams show ferritin expression in nonpermeabilized (thin lines, – saponin)...

Ngày tải lên: 19/02/2014, 16:20

... alter some cellular processes in a cell type-dependent man- ner. For instance, an increase in the cell cycle timing could be expected following the downregulation of PCNA, coding for a protein involved ... alone affected the expression of a few genes, especially in IMR-90 cells, such as IGFBP3 (insulin-like growth fac- tor-binding protein 3) (3.3-fold decrease), ICAM1 (intercel...

Ngày tải lên: 07/03/2014, 05:20

... stress. Thirteen genes, which encode six protein families responsible for protein folding, are included in the UPR gene list. Among them, BiP (three genes), calnex- in (two genes), calreticulin (two genes), ... 3461 vesicle trafficking ⁄ transport, vacuolar protein sorting, cell wall biogenesis, and lipid ⁄ inositol metabolism. In comparison with those of yeast, the UPR genes of mamm...

Ngày tải lên: 07/03/2014, 21:20

... °C for 7 min. PCR was analyzed by electrophoresis in a 0.9% agarose gel. Single cell RT-PCR of F-11 cells F-11 cells were diluted in 5 · RT first strand buffer to a concentration of 1 cell per ... parental cell line N18TG2. This finding is of interest when VRL-1 is functionally studied in these cells. Additionally in this study we cloned VRL-1 from total RNA isolated from rat...

Ngày tải lên: 31/03/2014, 07:20

... transgene efficiently. Induction of toxin expression led to cell death and development of cardiomyopathies. Stringent control of transgene expression using rtTA has also been achieved in HeLa cells ... and screening systems in E.coli [104,105] and in S. cerevisiae [88] have lead to a profound understanding of how DNA binding, inducer binding and dimerization function in TetR. T...

Ngày tải lên: 23/03/2014, 21:20

... which contain a fifth nucleotide. J Biol Chem 227, 907–915. 2 Koonin EV (1996) Pseudouridine synthases: four fami- lies of enzymes containing a putative uridine-binding motif also conserved in dUTPases ... pseudouridines were present at positions 1911 and 1917 in the wild-type 23S rRNA in the 50S subunits and in the 70S ribosomes. 23S rRNA from a strain lacking RluD did not have pseudo...

Ngày tải lên: 18/02/2014, 16:20

... protein from BL21(DE3) cells (B) or uninduced cells containing the pRSET-mCyp4x1 (U), the 10 000-g pellet from cells induced for 3 h with 1 mm isopropyl thio-b- D-galactoside (P), and the proteins ... the Cyp4x1 protein in brain showed specific staining of neurons, choroids epithelial cells and vascular endothelial cells. These data suggest an important role for Cyp4x1 in the brai...

Ngày tải lên: 19/02/2014, 07:20

... once in the same pattern. Different from conventional sequential pattern mining method, in feature combination mining for SVM only contains a set of feature vectors each of which is independently ... The training of SVMs involves in minimize the following object (primal form, soft-margin) (Vapnik, 1995): ∑ = +⋅= n i ii yxWLossCWWW 1 ),( 2 1 )( :minimize α (1) The loss function...

Ngày tải lên: 20/02/2014, 12:20

... pro- teins have roles in ‘managing’ stress tolerance and in maintaining redox homeostasis [15–18]. It is also note- worthy that a study, using microarray to examine the gene expression in colonic ... per 10 6 cells) , while free choline and glycerophosphocholine both increased by about three-fold. The changes in the levels of water- soluble choline-containing metabolites observed...

Ngày tải lên: 07/03/2014, 06:20