Báo cáo khoa học: The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain ppt

... 2877 The domains carrying the opposing activities in adenylyltransferase are separated by a central regulatory domain Paula Clancy 1 , Yibin Xu 1,2 , Wally C. van Heeswijk 1,3 , Subhash G. Vasudevan 1,4 and ... C-terminal domains in these types of proteins [10]. Together, these observations suggested that the opposing activities of the two domains m...

Ngày tải lên: 30/03/2014, 08:20

... study are interesting because they indicate that both domains are capable of causing respiratory uncoupling. These data suggest that eppin acts as an antibacte- rial agent capable of killing Gram-negative ... [32–34]. WAP domains in other proteins, however, have been shown to have protease inhibitory activity. For example, the C-terminal domain of avian WAP domain and Kunitz...

Ngày tải lên: 23/03/2014, 07:20

... Instead of considering FAA only as a part of the osmo- and cryoprotective arsenal, they should also be regarded as main actors involved in the multiple regulatory pathways activated during cold acclimation ... d-pyrolline-5-carboxylate which, in turn, can be oxidized to Glu. Transamination gave rise to Ala and a- ketoglutarate. Such an increase in Ala contents is necessary to s...

Ngày tải lên: 16/03/2014, 11:20

... scores of the pooled summaries? Kazawa et al. calculated the score of a summary as a weighted linear sum of the manual scores. Applying regression analysis (Yasuda et al., 2003) is another method ... arbitrary summaries from the 10 summaries in a dataset were selected and ranked by Kazawa’s method. Then, Kazawa’s method was evaluated using “Precision,” which calculat...

Ngày tải lên: 17/03/2014, 04:20

... study, the catalytic domains are indicated by capital letters ( A and B) and the C-terminal peptides by lower case letters (a and b), so that the wild-type enzymes are abbreviated as Aa and Bb. The ... protein or alkaline phos- phatase allowed the formation of tetramers associated with an N-terminal fragment of ColQ [17]. However, the catalytic domains are also in...

Ngày tải lên: 07/03/2014, 03:20

... the sequences in the database is about 60%. Hits between the N- and C-terminal halves Each of the sequences in the domain database was used as query in a blast search against all domains in the ... uses a database con- taining the N- and C-terminal halves of all the sequences in ST3 and involves blast searches of each sequence in the database against the...

Ngày tải lên: 07/03/2014, 17:20

... domains. In one struc- ture, the catalytic cysteines face each other; in the other, the catalytic residues of the a domain face away from the a domain. The crystal structures also revealed the presence ... cysteines in the C-terminal a domain increased the K m to 30 lm, and loss of an additional cys- teine in the a domain resulted in an increase of...

Ngày tải lên: 16/03/2014, 04:20

... expression of the Nogo -A fragments The Nogo -A cDNA (designated KIAA 0886) was obtained from the Kazusa DNA Research Institute (Kazusa- Kamatari, K isarazu, Chiba, Japan). A DNA fragment encoding a 182 ... doma in organization o f the hum an Nogo -A protein. (A) T he do main o rganizat ion of human Nogo -A s howing the N -terminal stretch region Nogo -A( 567–748) and the...

Ngày tải lên: 16/03/2014, 16:20

... the barbed end and sides of actin filaments [15–18]. These actin-binding activities are conserved in the EVH2 domain [19,20]. On the other hand, the EVH1 domain binds to proteins containing the ... Pro-rich domains, also supporting the con- clusion that the EVH2 domain is the RAD51-binding domain in EVL. RAD51 and actin, which both bind ATP, may share a commo...

Ngày tải lên: 23/03/2014, 04:20

... according to the instructions provided with the kit: K29 9A: 5¢-GTTATTAAACGTGTTGCATATAATAAC GAG-3¢; DM: 5¢-ACTTACGTTATTGCACGTGTTGCAT ATAATAACGAG-3¢; hep2: 5¢-CTGAAAAATGATGGAG AGCTCACAGAGAGTAAGGGAACTTTG-3¢. The ... amino- terminal heparin-binding domain (ATD) overlaps with the ATP-binding catalytic subdomain II and the carb- oxy-terminal heparin-binding domain (CTD) is located be...

Ngày tải lên: 23/03/2014, 13:20