Báo cáo khoa học: Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum pdf

... gliadain in maturing and germinating wheat seeds. (A) Northern blotting analysis of gliadain in maturing and germinating seeds. Aliquots of 10 lg of total RNA were loaded into each lane. Maturation stage ... and days after imbibition. (C) Western blotting analysis of gliadain in germinating seeds. Anti-gliadain serum was raised against GST–progliadain. Numbers indicate...

Ngày tải lên: 30/03/2014, 08:20

... C-terminal adenylation domain A 4 again is predicted to activate l-arginine, displaying 60% identity to the characterized A- domain of MycC. Interestingly, A 1 and A 4 inherit a highly identical (90%) ... 1813–1815. 33 Aoyagi T, Wada T, Iinuma H, Ogawa K, Kojima F, Nagai M, Kuroda H, Obayashi A & Umezawa H (1985) In uence of angiotensin-converting enzyme inhibitor, foroxym...

Ngày tải lên: 16/02/2014, 09:20

... h + A 30 min PAGE Pipet to plate, add A beads and incubate +16 h D 1 h Autoradiography Add D beads and incubate D A O 2 Read AlphaLISA si g nal at 615 nm HSE Fig. 1. Comparison of EMSA and TransLISA ... TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses Kristiina A. Vuori 1 , Johanna K. Ahlskog 2 , Lea Sistonen 2 and Mikko Nikinmaa 1...

Ngày tải lên: 18/02/2014, 14:20

... recognition. In recent years, a number of studies have inves- tigated integrating emotions and music in certain media applications. For example, Ishizuka and Onisawa (2006) generated variations of theme ... We also integrate the sentiment-detection system with a real-time rule-based harmonic music and animation generator to display streams of messages in an audiovisual fo...

Ngày tải lên: 20/02/2014, 05:20

... further data collec- tion projects conducted in a similar manner. 1 Introduction Automatic question answering (QA) is an alternative to traditional word-based search engines. Instead of returning a ... multiple answers and corresponding articles, but also certain additional information that we believe is essential to enhance the usability of the data. In addition to providing a...

Ngày tải lên: 20/02/2014, 09:20

... a probability to every way in which a source e can be mapped into a tar- get f. Once the parameters of the language and translation models are estimated using traditional maximum likelihood and ... trans- lation memory and a statistical-based translation model. Our results show that an automatically derived transla- tion memory can be used within a sta- tistical framework to oft...

Ngày tải lên: 17/03/2014, 07:20

... pre- sented in Fig. 3C suggest increases in cytosolic [Ca 2+ ] in response to CsA and its analogs. Both the intra- cellular Ca 2+ chelator BAPTA and the extracellular Ca 2+ chelator EGTA caused significant ... FL500 fluorescent plate reader. Statistical analysis Data were analyzed using prism 4 for Windows (GraphPad Software, Inc., San Diego, CA). Two-way ANOVA was used for assessment of...

Ngày tải lên: 30/03/2014, 08:20

... Ltd), a SLC3 9A1 4-specific forward primer: 5¢-CCCACTCAGTAGCTGTGT-3¢,5¢-CAATGCTGGCAT GAGCAT-3¢ or 5¢-CTTCTTGGGGAAACATG-3¢, and a reverse primer: 5¢-CCAGCATAATGGAGAAGC-3¢,5¢-AA CTGGACCCTAAGCCTA-3¢ ... to a Marathon cDNA adapter by T4 DNA ligase. The PCR for 5¢-RACE was performed using the for- ward primer AP-1: 5¢-CCATCCTAATACGACTCACTAT AGGGC-3¢ and a SLC3 9A1 4-specific reverse primer:...

Ngày tải lên: 30/03/2014, 16:20

... 16 Wakabayashi H, Matsumoto H, Hashimoto K, Teragu- chi S, Takase M & Hayasawa H (1999) Inhibition of iron ⁄ ascorbate-induced lipid peroxidation by an N-ter- minal peptide of bovine lactoferrin ... of tyrosine sulfate containing peptides can be challenging because of possible hydrolysis of the sulfate residue occurring during synthesis, in Table 1. Eugenin, and mammalian an...

Ngày tải lên: 19/02/2014, 16:20

... transcription complexes. Abbreviations ABLIM, actin-binding LIM protein; ABP, actin-binding protein; ANF, atrial natriuretic factor; AR, androgen receptor; CARM1, coactivator- associated arginine methyltransferase ... transfection data indicate that a -actinin-4 potentiates transcrip- tional activity by MEF2. Second, overexpression of a- actinin-4 decreases the interaction of MEF 2A a...

Ngày tải lên: 07/03/2014, 01:20