... of1mLÆmin)1, with a linear gradient from 100% B to 43% Bwithin 45 min, and then to 0% within 1 min, with the finalconditions being maintained for another 24 min at a flowrate of 2 mLÆmin)1. ... trans-formant strains were named T3 and T4.The molecular integrity of carD was investigated in T3 and T4 strains, in two nonsectoring transformants,T1 and T2, and in the SF134 original strain. ... were investigated. As a reference, carB,coding for the phytoene desaturase, was analyzed in parallel. As shown in Fig. 3, carD mRNA levelsincreased about three-fold after 30 min of illumination,and...