Báo cáo khoa học: Thermal unfolding and aggregation of actin

Báo cáo khoa học: Thermal unfolding and aggregation of actin Stabilization and destabilization of actin ﬁlaments doc

... whereas opening of the cleft leads to significant destabilization of G -actin. Thermal unfolding of F -actin filaments Polymerization of G -actin to F -actin The use of DSC allows very clear probing of the changes ... properties of actin filaments. Biophys J 82, 321–334. 40 Bertazzon A & Tsong TY (1990) Effects of ions and pH on the thermal stability of...

Ngày tải lên : 30/03/2014, 04:20

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Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... the thermal unfolding of non- actin- bound Tm in the presence of F -actin, and peak 3 corresponds to the thermal unfolding of free Tm dur- ing re-heating after complete irreversible denaturation of ... thermal unfolding of smTm, Tm5a, and Tm5b complexed with F -actin. DSC experiments with Tm–F -actin complexes were performed in the presence of excess of Tm, i....

Ngày tải lên : 19/02/2014, 07:20

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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... width of 1 nm, response time of 4 s, and scan speed of 50 nmÆs )1 . Isothermal guanidine hydrochloride denaturation studies Denaturant-dependent equilibrium unfolding studies were done by CD and ... 2007 FEBS Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the...

Ngày tải lên : 16/03/2014, 10:20

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Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt

... we noticed excellent chaperone-like activity of MTP. MTP prevents chemical and thermal aggregation of other proteins and can enhance the extent of refolding of fully unfolded substrate enzymes. Because ... results of investigation of chaperone-like activity of MAP2 using dithiothreitol-induced aggregation of insulin at 25 °C. Here, the inhibition of aggregation i...

Ngày tải lên : 23/03/2014, 12:20

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Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... widespread and central class of sexual development factors in ﬁsh Amaury Herpin and Manfred Schartl Physiological Chemistry I, University of Wuerzburg, Germany Introduction The phenomenon of two ... Considering that dmrt1bY has all the features of the master regula- tor of testicular differentiation in medaka (see [13] for review) and because of the discovery of sex-chromo...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy in living cells doc

... Subcellular localization and enzymatic activities of GAPDH– citrine and PGK–cerulean. (A) Expression of GAPDH–citrine and PGK–cerulean. Localization of endogenous GAPDH and PGK in HeLa cells was ... tetrameric structure of GAPDH is schematically shown on the right. The distances between the N- terminal amino acids of subunits O and P, subunits O and Q and subunits O a...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... sum up some of the available data and mechanisms identiﬁed in leukemic stem and leukemic tumor cells and link this informa- tion with the known functions of mixed lineage leukemia and certain ... for example, HoxA7 and HoxA9. The transforming capac- ity of the tested MLL fusion constructs was also dependent on the presence of Meis1 and Pbx proteins, as well as on the prese...

Ngày tải lên : 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: The two Caenorhabditis elegans metallothioneins (CeMT-1 and CeMT-2) discriminate between essential zinc and toxic cadmium docx

... CeMT-1 and CeMT-2. When equimolar amounts of Zn(II) and Cd(II) are used (0.65 lm each), 93% of Zn(II) is bound to CeMT-1, and 85% of Cd(II) is bound to CeMT-2. With a 10-fold excess of MTs and the ... Deconvoluted ESI mass spectra of the various metalloforms of Caenorhabd- itis elegans MTs. Holo zinc (A) and cadmium (B) species of CeMT-1; zinc (C) and cadmium (D) s...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc

... structure and evolution of the DNAzymes. The regulating effects of RS and RNase on the DNA cleavage of DRc DNAzyme After characterizing the DNA-cleaving and RNA- cleaving activities of DRc DNAzyme, ... 2¢,3¢-cyclic phosphate and the 5¢-hydroxyl termini of RNA fragments [22]. In its catalytic core, the dinucleotides A6G7 of a terminal AGC loop and C13G14 of a bulge lo...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... is a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ... structure and the ﬂexibility of Zn(II) and Cd(II) by NMR spectroscopy. Another interesting (and to my knowledge not yet reported) experiment would be assessment of the growth-inhibi-...

Ngày tải lên : 16/02/2014, 15:20

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