Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf

... targets, the reconstruction of pathways and help to understand the mechanism of action and side-effects of therapeutic compounds. Correspondence to B. Kuster, Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, ... activity of the spliceosome is the recognition of splice sites in nuclear pre-mRNA and to catalyze the accurate removal of introns and ligation of exons to yiel...

Ngày tải lên: 31/03/2014, 07:20

... and anandamide oxidation FEBS Journal 275 (2008) 3706–3717 ª 2008 The Authors Journal compilation ª 2008 FEBS 3717 Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide Katarina ... assembly. Fig. S2. Comparison of rat P450 4X1 and mouse P450 4x1 microsomal sequences with human P450 4X1. Fig. S3. Steady-state kinetic...

Ngày tải lên: 30/03/2014, 04:20

... both directly and through activation of PLA2 ) may trigger enhanced mitochondrial production of superoxide and hydrogen peroxide, and cause the release of cytochrome c. Materials and methods Materials Chemicals ... and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Portugal; 3 James Graham Brown Cancer Center, University of Lou...

Ngày tải lên: 31/03/2014, 07:20

... for an understanding of the physiological role of epoxides of fatty acids in plants. In addition to cytochromes P450, two distinct types of plant enzyme, unrelated to cytochrome P450, with epoxidase ... expression of CYP77A4 in yeast For functional expression of the full-length CYP77A4 clone, we used a yeast expression system specifically developed for the expression o...

Ngày tải lên: 07/03/2014, 03:20

... of internal electron transfer in the presence of NADPH, NADH and fragments of nicotinamide coenzymes. We show that binding of the 2¢-phosphate of NADPH, and to a lesser extent other regions of ... relaxation spectroscopy. Members of the cytochrome P450 mono-oxygenase super- family catalyse the hydroxylation of a wide range of physiological and xenobiotic compounds;...

Ngày tải lên: 23/03/2014, 17:22

... Kinetic analysis of isoaspartyl peptidase activity of (a) EcAIII and (b) LlA. 3226 D. Borek et al. (Eur. J. Biochem. 271) Ó FEBS 2004 Expression, purification and catalytic activity of Lupinus luteus asparagine ... of Sciences, Poznan, Poland; 3 Molecular Medicine Unit, University of Leeds, UK; 4 Laboratory of Protein Engineering, Institute of Biochemistry and Molecular...

Ngày tải lên: 30/03/2014, 15:20

... Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein Alinda Nagy, Ma ´ ria Trexler and La ´ szlo ´ Patthy Institute of ... in ovary, testis and brain, the most significant expression of the WFIKKN gene is observed in adult pancreas, liver and thymus. In view of the presence of WAP-, Kazal-, Kunitz-...

Ngày tải lên: 31/03/2014, 01:20

... generous gift of RhoA DNA constructs and Dr L. Navarro and Dr J.E. Dixon (UCLA, USA) for sending us YpkA wild-type and D267A expression plasmids. We also thank Dr C. Wright (University of Oxford, ... Kessler Department of Clinical Medicine, University of Oxford, UK Introduction The genus Yersinia consists of three pathogenic species that are agents of a variety of diseas...

Ngày tải lên: 16/02/2014, 15:20

... and secretion of IL-10 Because of the lack of secretion of the inflammatory cytokines IL-1b and TNF-a from hypoxia ⁄ SD-stimu- lated MSCs, as well as the significant anti-inflamma- tory effects of MSCs, expression ... presence and absence of IL-10. *P < 0.05 and **P < 0.01 versus Cont group. (D) Representative western blots for collagen I and III in the presence an...

Ngày tải lên: 18/02/2014, 04:20

... Sciences, Turku, Finland). Estimation of expression of cytosolic regulatory proteins Total cell lysates of transfected CHO cells were used to esti- mate expression of Noxo1b, Noxo1c, and Noxo1a. The lysates ... 3673 Expression of Noxo1b and Noxo1c in various human tissues The expression patterns of the Noxo1b and Noxo1c mes- sengers were determined by PCR using Human...

Ngày tải lên: 19/02/2014, 06:20