Báo cáo khoa học: Brassinin oxidase, a fungal detoxifying enzyme to overcome a plant defense – purification, characterization and inhibition potx

... napus and Brassica rapa) and vegetables such as cabbage (Brassica oleraceae var. capitata), cauliflower (Brassica oleraceae var. botrytis) or broccoli (Brassica oleraceae var. italica). In addi- tion, ... FEBS 3705 Brassinin oxidase, a fungal detoxifying enzyme to overcome a plant defense – purification, characterization and inhibition M. S. C. Pedras, Zora...

Ngày tải lên: 30/03/2014, 04:20

... inter- action of RNase A and 3¢-nucleotides, and indicate that non-natural fura- nose rings can serve as the basis for more potent inhibitors of catalysis by RNase A. Abbreviations araUMP, arabinouridine ... (dU F MP) and arabinouridine 3¢-phosphate (araUMP) have non-natural furanose rings. dU F MP and araUMP were prepared by chemical synthesis and found to have three- to se...

Ngày tải lên: 16/03/2014, 18:20

... twice; that is, if Ala25 of chain A is close to Asn77 of chain B, then Asn77 of chain A is close to Ala25 of chain B. Chain A Chain B Hydrogen bonds Ala25 Asn77, Arg80 AlaO–ArgNH1 AlaO–ArgND2 Asn26 ... diffracts to a Table 1. Statistics on data collection and refinement. A wavelength of 0.8726 A ˚ was used. Rotations of 1° were performed. The Ramachan- dran plot was calcula...

Ngày tải lên: 16/02/2014, 14:20

... F. Barr and colleagues [35]. GRASP55F in pCDNA3.1 Zeo+ con- tained a C-terminal FLAG tag. Full-length GRASP55, GRASP55 PDZ1 (amino acids 1–1 07), GRASP55 PDZ2 (amino acids 8 4–1 72) and GRASP55 region ... 140 6–1 414. 54 Itoh Y, Takamura A, Ito N, Maru Y, Sato H, Suenaga N, Aoki T & Seiki M (2001) Homophilic complex for- mation of MT1-MMP facilitates proMMP-2 activation on th...

Ngày tải lên: 18/02/2014, 04:20

... mem- brane can lead to a more complete understanding of the membrane-aided assembly of Ab and the resulting damage to cell membranes. J. A. Lemkul and D. R. Bevan Membrane perturbation by Alzheimer’s ... the presence of rat synaptic plasma membranes has shown that monomeric Ab can intercalate into the bilayer interior and lead to decreased bilayer thick- ness [7]. The same stud...

Ngày tải lên: 18/02/2014, 08:20

... -ATC ATC TCC ATC GAC TAC TCC CTG-3¢, the antisense primer 5¢-AAG AAT TCT AGA TTA ATG GTG ATG ATG GTG ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... accelerating voltage (Jeol, Tokyo, Japan). Images were recorded with a Gatan Multiscan 791 CCD camera (Gatan UK, Abingdon, UK) [23]. Acknowledgements We would like to thank B. Danielsson and...

Ngày tải lên: 18/02/2014, 11:20

... Arg316 and Glu275 residues Xiaohui Liu, Ayami Matsushima, Hiroyuki Okada, Takatoshi Tokunaga, Kaname Isozaki and Yasuyuki Shimohigashi Laboratory of Structure–Function Biochemistry, Department ... recognized as an estrogenic chemical able to interact with human estrogen receptor (ER) [ 1–3 ], and recently was reported also to act as an antagonist for a human androgen receptor (A...

Ngày tải lên: 18/02/2014, 16:20

... lipid -to- protein ratios of 0.5 and 1.0 assembled into 2D crystals that were exam- ined by high-resolution atomic force microscopy (AFM). AFM topographs revealed crystalline and paracrystalline membrane patches ... ATP synthase – a marvellous rotary engine of the cell. Nat Rev Mol Cell Biol 2, 66 9–6 77. 4 Sambongi Y, Iko Y, Tanabe M, Omote H, Iwamoto- Kihara A, Ueda I, Yanagida...

Ngày tải lên: 18/02/2014, 17:20

... growth factors and proteinase inhibitors. Biol Chem 380, 47 3–4 83. 17 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- sis of tissue factor and tissue ... the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the extracellular matrix...

Ngày tải lên: 19/02/2014, 00:20

... the samples was assayed according to Bradford [28], with BSA as the standard. Extraction and analysis of cytokinins Two grams of frozen plant material (barley kernels, 7- and 14-day-old barley ... of barley and demonstrate functionality for one of them in transgenic tobacco an d Arabidopsis plants. Materials and methods Plant materials Commercial barley (H. vulgare cv. Luxor) a...

Ngày tải lên: 19/02/2014, 16:20