Báo cáo khoa học: Dynamics, stability and iron-binding activity of frataxin clinical mutants pdf

... frataxin mutants A. R. Correia et al. 3690 FEBS Journal 275 (2008) 3680–3690 ª 2008 The Authors Journal compilation ª 2008 FEBS Dynamics, stability and iron-binding activity of frataxin clinical mutants Ana ... performed a comparative study of the protein dynamics of frataxin variants carrying mutations of clinical interest. We focused on how the frataxin mut...

Ngày tải lên: 30/03/2014, 04:20

... degradation of the poly(A) tails of single-stranded mRNAs in a highly processive mode. PARN participates in diverse and important intracellular processes by act- ing as a regulator of mRNA stability and ... bind with the 3¢-poly(A) tail and the 5¢-cap of the mRNA, and may be important to the allosteric regulation of PARN [19–23]. In general, the structure and stability...

Ngày tải lên: 23/03/2014, 04:21

... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5. Structure of cyclic hexapeptides – cYT peptide. The NMR data of the cYT peptide wer e indicative of its flexible nature. ... structure of peptide cER consists of two b-turns, located at the N- and C-termini. A comparison of the b-turn structure of cER with the similar region in the crysta...

Ngày tải lên: 19/02/2014, 13:20

... specific 5¢ -and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no. D11350 and S70040). Primers for the short isoform of b-fructofuranosidase ... was calculated as percent of the total amount of histamine determined after lysis of the basophils by twofold freezing and thawing of the cells. A histamine release of...

Ngày tải lên: 21/02/2014, 00:20

... of the horseshoe crab Limulus polyphemus,to form a solid gel in the presence of minute amounts of endotoxins. The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of ... water-binding capa- city of lecithin headgroups [30]. Finally, MfGl-II exhibits a main band at 1245 cm )1 and further weak bands at 1220 and 1260 cm )1 . Thus, the phosphate grou...

Ngày tải lên: 21/02/2014, 00:20

... 4827 MINIREVIEW Efficient and targeted delivery of siRNA in vivo Min Suk Shim 1 and Young Jik Kwon 1,2,3 1 Department of Chemical Engineering and Materials Science, University of California, Irvine, ... with a strand of siRNA, and the other siRNA strand was subsequently annealed to the aptamer-conjugated strand. These aptamer–siRNA conjugates were able to access HIV- infected c...

Ngày tải lên: 06/03/2014, 01:23

... processing and evaluation were performed using the origin 7.5 software. Crystallization, data collection and refinement of the E542K and L537G mutants Crystals of the E542K, L537G and E542K ⁄ L537W mutants ... mm )1 Æcm )1 ). One unit of P2Ox activity was defined as the amount of enzyme necessary for oxidation of 2 lmolÆmin )1 of ABTS (which equals the consumption of...

Ngày tải lên: 07/03/2014, 03:20

... mimic some of the structural and functional proper- ties of the native proteins and thus may offer a useful basis for the design of agents for therapeutic interven- tion [7]. Studies of different ... effect of the lipid composition on KL 4 struc- ture, in the absence and presence of Ca 2+ , using CD and (c) the role of the lipid composition and peptide structure on sur...

Ngày tải lên: 30/03/2014, 11:20

... [15]. Analysis of a structural model of AmFd suggests that a combination of additional sur- face ion pairs, the zinc cofactor, and an efficiently packed core govern the high stability of this protein [36]. ... are similar [55]. The stability profiles of TkRNase HII and TtRNase HII are similar, although TkRNase HII exhibits a higher temperature of maximum stability and is...

Ngày tải lên: 07/03/2014, 05:20

... dis- tribution of the energy between the overall tumbling and the internal mobility, and is indicative of the degree of internal restraint of each 15 N– 1 H vector motion. In Fig. 4, most of the points ... graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relax...

Ngày tải lên: 18/02/2014, 16:20