Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx

... Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content Betu ¨ l T. Yesilyurt 1 , Constant ... 2008) doi:10.1111/j.1742-4658.2008.06507.x The thermal stability of the eight functional units of b -hemocyanin of the gastropodan mollusc Helix pomatia was investigated by FTIR spect...

Ngày tải lên: 30/03/2014, 04:20

... material is available as part of the online article from http://www.blackwell-synergy.com Please note: Wiley-Blackwell are not responsible for the content or functionality of any supplementary materials ... of patients characterized. In preliminary studies, we addressed the question of whether prevalent mutations that result in classical FRDA phenotypes were correlated with comp...

Ngày tải lên: 30/03/2014, 04:20

... T (2005) Functional importance of Ca 2+ -deficient N-terminal lobe of molluscan troponin C in troponin regulation. Arch Biochem Biophys 436, 83–90. H. Tanaka et al. Functional regions of molluskan ... (1971) The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin–troponin complex with actin and the proteolytic fragmen...

Ngày tải lên: 20/02/2014, 01:20

... comparison of Tm5a with smTm allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscle exon 1b, and comparison of Tm5a with Tm5b allows the effects of exchange of exon 6 ... presence of exon 1b dramatically decreases the measurable calorimetric enthalpy of the thermal unfolding of Tm observed with DSC, although it has no influence on the a -h...

Ngày tải lên: 19/02/2014, 07:20

... between 300 and 550 nm with a scanning speed of 150 nmÆs )1 and an excitation slit of 5.0 nm. Calorimetric measurements Thermal analysis of protein denaturation was performed with DSC (a model 6100 ... terms of assembly of fragments is discussed. Results CD spectra Secondary structure of SNase and its mutants was deter- mined by CD with spectrapolorimetry measurements. The...

Ngày tải lên: 20/02/2014, 01:20

... provide information Helix H Helix G Helix EHelix D Helix A Helix B Heme Helix C Helix F C C D B G H F N A E Fig. 1. Three-dimensional structure (top) and amino acid sequence (bottom) of human Ngb. ... thermophilic Aquifex aeolicus [54]. Functional stability of Ngb The conformational stability of the apoNgb structure in acid is in agreement with the enhanced functi...

Ngày tải lên: 07/03/2014, 02:20

... than BsCsp (T M of 50 °C, pH 7; DG U of 11 kJÆmol )1 ,pH7, 20 °C) [44]. This difference in thermodynamic stability correlates with two orders of magnitude slower unfold- ing of TmCsp as compared ... [15]. Analysis of a structural model of AmFd suggests that a combination of additional sur- face ion pairs, the zinc cofactor, and an efficiently packed core govern the high stab...

Ngày tải lên: 07/03/2014, 05:20

... while most of the mutations of negatively charged glutamic acid or aspartic acid residues to uncharged glycine resi- dues do not disrupt the secondary structure of SNase, replacement of E129 with G ... pro- tein secondary structure. However, this replacement of G at E129 does not significantly influence its whole protein stability (see Results of thermal analysis sec- ti...

Ngày tải lên: 07/03/2014, 21:20

... of D276 and N305 in rat HDC) are predicted to interact with the imidazole ring and the phosphate group of PLP, respectively [18,36,37]. It is noteworthy that in spite of the high flexibility of ... inhibitor was not able to protect the enzyme against thermal denaturation. The increased resistance of the a-FMH-treated protein to thermal denaturation would indicate that the cov...

Ngày tải lên: 08/03/2014, 08:20

... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV melting curves, from which the temperature of denaturation (T d ) and the van’t Hoff enthalpy of denaturation (DH° vH )of1 7b-HSDcl were ... pH > 10. Of note, the observed thermody- namic stability of 17b-HSDcl at 25 °C, with a value of 15.3 kJÆmol )1 (0.06 kJÆmol )1 amino acid), is much lower than for the m...

Ngày tải lên: 16/03/2014, 13:20