Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx
Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx
... Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content Betu¨l T. Yesilyurt1, Constant ... 2008)doi:10.1111/j.1742-4658.2008.06507.xThe thermal stability of the eight functional units of b -hemocyanin of thegastropodan mollusc Helix pomatia was investigated by FTIR spectros-copy. Molluscan hemocyanin functional units have ... the functional units of H. pomatia b -hemocyanin, designated HpH-a to HpH-h, differ considerablyin their carbohydrate content (0–18 wt%). Most functional units are excep-tionally stable with...
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Báo cáo khoa học: Dynamics, stability and iron-binding activity of frataxin clinical mutants pdf
... material is available as part of the online articlefrom http://www.blackwell-synergy.comPlease note: Wiley-Blackwell are not responsible forthe content or functionality of any supplementarymaterials ... of patients characterized.In preliminary studies, we addressed the question of whether prevalent mutations that result in classicalFRDA phenotypes were correlated with completeimpairment of ... and animpairment of the biosynthesis of iron-sulfur proteins[1–4]. The majority of FRDA patients (> 95%) arehomozygous for a GAA repeat expansion within thefirst intron of the frataxin gene...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... T (2005) Functional importance of Ca2+-deficient N-terminallobe of molluscan troponin C in troponin regulation.Arch Biochem Biophys 436, 83–90.H. Tanaka et al. Functional regions of molluskan ... (1971) The regulation of rabbitskeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin–troponin complex with actin and the proteolytic fragments of myosin.J Biol ... 375–380. Functional regions of molluskan TnI H. Tanaka et al.4486 FEBS Journal 272 (2005) 4475–4486 ª 2005 FEBSComparative studies on the functional roles of N- andC-terminal regions of molluskan...
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Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx
... comparison of Tm5a with smTm allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscleexon 1b, and comparison of Tm5a with Tm5b allowsthe effects of exchange of exon 6 ... presence of exon 1b dramatically decreases the measurablecalorimetric enthalpy of the thermal unfolding of Tm observed with DSC,although it has no influence on the a -helix content of Tm or on ... enthalpy of free Tm (DH3) with the enthalpy of peak 1 subtracted (DH3) DH1)(Table 2). (It is noteworthy that in the absence of actinthe enthalpy of reversible unfolding of Tm did not sig-nificantly...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... between 300 and 550 nm with a scanning speed of 150 nmÆs)1and an excitation slit of 5.0 nm.Calorimetric measurements Thermal analysis of protein denaturation was performed with DSC (a model 6100 ... terms of assembly of fragments is discussed.ResultsCD spectraSecondary structure of SNase and its mutants was deter-mined by CD with spectrapolorimetry measurements.The approximate fraction of ... thesecondary structure of the protein disintegrated. Usingthe peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...
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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... provide information Helix H Helix G Helix EHelix D Helix A Helix BHeme Helix C Helix FCCDBGHFNAEFig. 1. Three-dimensional structure (top) and amino acid sequence(bottom) of human Ngb. ... thermophilicAquifex aeolicus [54]. Functional stability of NgbThe conformational stability of the apoNgb structurein acid is in agreement with the enhanced functional acid stability of the holoprotein. In ... estimate of the stability of the Ngb fold at acidicpH was obtained by measuring the urea-mediatedFig. 4. Proteolysis of apoNgb and apoMb with pepsin. Proteolysis of the proteins by pepsin (E ⁄ S of...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... thanBsCsp (TM of 50 °C, pH 7; DGU of 11 kJÆmol)1,pH7,20 °C) [44]. This difference in thermodynamic stability correlates with two orders of magnitude slower unfold-ing of TmCsp as compared ... [15]. Analysis of a structural model of AmFd suggests that a combination of additional sur-face ion pairs, the zinc cofactor, and an efficientlypacked core govern the high stability of this protein[36]. ... Extrapolation of kineticdata in the presence of denaturants suggests thatunfolding of the hyperthermostable Fds at pH 7 inbuffer (20 °C) requires hundreds of years [35]. For the homologous [2Fe)2S]...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... whilemost of the mutations of negatively charged glutamicacid or aspartic acid residues to uncharged glycine resi-dues do not disrupt the secondary structure of SNase,replacement of E129 with G ... pro-tein secondary structure. However, this replacement of G at E129 does not significantly influence its wholeprotein stability (see Results of thermal analysis sec-tion below).Tryptophan fluorescence ... interactions of G75 with H121 and K9, and of G129 with K110 and K133. Other chargedamino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129.H M. Chen et al. Local stability...
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Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... of D276 and N305 in rat HDC) arepredicted to interact with the imidazole ring and thephosphate group of PLP, respectively [18,36,37]. It isnoteworthy that in spite of the high flexibility of ... inhibitor was not ableto protect the enzyme against thermal denaturation.The increased resistance of the a-FMH-treated protein to thermal denaturation would indicate that the covalentbinding of the ... adduct within the catalytic centerin addition to those established by the internal aldiminealone. From the shape of the a-FMH-treated proteinspectra, stabilization of some a -helix by the cofactor...
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Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx
... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV meltingcurves, from which the temperature of denaturation(Td) and the van’t Hoff enthalpy of denaturation(DH°vH )of1 7b-HSDcl were ... pH > 10. Of note, the observed thermody-namic stability of 17b-HSDcl at 25 °C, with a value of 15.3 kJÆmol)1(0.06 kJÆmol)1amino acid), is muchlower than for the majority of globular ... concentrations of 1.2and 0.4 m, respectively. Binding of the coenzyme NADPH to 17b-HSDclcauses local structural changes that do not significantly affect the thermal stability of this protein.AbbreviationsCd,...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015