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Báo cáo khoa học: Interaction with model membranes and pore formation by human stefin B – studying the native and prefibrillar states docx

Báo cáo khoa học: Interaction with model membranes and pore formation by human stefin B – studying the native and prefibrillar states docx

Báo cáo khoa học: Interaction with model membranes and pore formation by human stefin B studying the native and prefibrillar states docx

... 2008 FEBS Interaction < /b> with < /b> model < /b> membranes < /b> and < /b> pore < /b> formation < /b> by< /b> human < /b> stefin < /b> B studying the native and < /b> prefibrillar states Sabina Rabzelj1,*,†, Gabriella Viero2,*,à, Ion Gutie´rrez-Aguirre3, ... MA. Pore < /b> formation < /b> of stefin < /b> B into lipid membranes < /b> S. Rabzelj et al.2466 FEBS Journal 275 (2008) 245 5–2 466 ª 2008 The Authors Journal compilation ª 2008 FEBS Interaction < /b> with < /b> model < /b> membranes < /b> and < /b> ... a basic building block of the cellular membranes,< /b> and < /b> negatively charged lipids Pore < /b> formation < /b> of stefin < /b> B into lipid membranes < /b> S. Rabzelj et al.2456 FEBS Journal 275 (2008) 245 5–2 466 ª 2008 The...
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx

Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx

... FABP 1B (ZF Fabp 1B, DQ062096), FABP2 (ZF Fabp2,AAH75970), FABP3 (ZF Fabp3, NP_694493), FABP7A (ZF Fabp7A,NP_571680) and < /b> FABP 7B (ZF Fabp 7B, AAQ92970) and < /b> human< /b> FABP1 (Hu FABP1, M10617), FABP2 ... FABP 1B; DQ062096), FABP2 (ZF FABP2; AAH75970), FABP3 (ZF FABP3; NP_694493), FABP7A (ZF FABP7A; NP_571680), FABP 7B (ZFFABP 7B; AAQ92970), and < /b> human < /b> FABPs FABP1 (Hu FABP1; M10617), FABP2 (HU FABP2; ... Fabp6 sequence with< /b> mammalian FABP6 sequences (human,< /b> rat, mouse and< /b> pig) and < /b> to paralogs of other zebrafish Fabps and< /b> human < /b> FABPs showed that zebrafish Fabp6 sharedgreatest sequence identity with...
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Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx

Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx

... with < /b> riboflavin, the proteinwas cocrystallised with < /b> riboflavin, and < /b> the crystals weresubsequently soaked with < /b> riboflavin. Cocrystallizationexperiments with < /b> the substrate analogue NORAP a nd the product ... organismsstudied [ 6–8 ,11], the enzyme from S. pombe binds riboflavin with < /b> relatively high affinity. This is believed to be due to ap-complex formation < /b> between the bound ligand and < /b> the adjacent tryptophan ... A˚[5],Fig. 7). In the W27Y mutant structu re with < /b> boundriboflavin, however, the Ca-trace dev iates from the otherstructures by < /b> 0.6 A˚, and < /b> the aromatic r ing is very fl exible. In the substrate a...
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Báo cáo khoa học: Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm pdf

Báo cáo khoa học: Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm pdf

... co-activators[34,35].TORCswereshowntobindtotheb-ZIPdomainofCREB, the binding being i ndependent of the Ser13 3– phosphorylation of C REB, and < /b> a ctivate CREB-mediatedtranscription. On the other hand, SIK1 was shown ... CAGGTCTG; the Bsp120I site is indicated by < /b> the underlined region). The cDNA fragments for the N -terminal regions and < /b> t heC-terminal regions were digested with < /b> BamHI/Bsp120I and < /b> b y EcoRI/Bsp120I, ... the N-terminal regions and < /b> of the C-terminal regions were separately amplified by < /b> PCR and < /b> then ligated to these fragments. To amplify frag-ments c ontaining residues 1–5 72, 1–5 85 and < /b> 1–6 12, the common...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc

Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc

... that the membrane might bedestabilized simply by < /b> surface interactions. However, the permeabilization by < /b> stefin < /b> B prefibrillar oligomersof vesicles made of acidic phospholipids resembles pore< /b> formation < /b> ... human< /b> stefin < /b> preparations. Cell viability was measured by < /b> the MTS test.Cells were exposed overnight to native stefin < /b> A (pH 4.8), native stefin < /b> B (pH 7.3) and < /b> to prefibrillar aggregates of stefin < /b> B, both, ... toxicityFEBS Journal 272 (2005) 304 2–3 051 ª 2005 FEBS 3051 Interaction < /b> of human < /b> stefin < /b> B in the prefibrillar oligomericform with < /b> membranes< /b> Correlation with < /b> cellular toxicityGregor Anderluh1,...
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Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx

Báo cáo khoa học: Interaction of the P-type cardiotoxin with phospholipid membranes pptx

... at the membrane/water interface deepening the side-chains of hydrophobic residues at the tips of the loops 1–3 down to the boundary between the glycerol and< /b> acyl regions of the bilayer; (c) the ... viscotoxin and < /b> delta-haemo-lysin [37,38]. The data from this suggest that the modes ofCTII interaction < /b> with < /b> the membranes < /b> can be described asfollows (Table 1).Mode 1: Attracted to the membrane ... component in the spectra is shown with < /b> broken line, the isotropic signal is truncated. (B) The amount of the isotropic signal plotted vs. pH and < /b> approximated by < /b> the best nonlinear fit for the determination...
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Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx

Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx

... follows: bands in the regions 169 6–1 680 cm)1 and < /b> 167 0– 1660 cm)1, b- turn; band at 1672 ± 2 cm)1, antiparallel b- sheet; band at 1651 ± 3 cm)1, a-helix; band at 1640 ±2cm)1, random coil; bands ... PtdIns 3-PNI Fatty acidsMyristic acid 1.0 Palmitic acid 0.75 Stearic acid 2.0 Lipoproteins and < /b> plasma proteins Human < /b> plasma 1 : 8a Human < /b> LDL 90 15 40Myoglobin b NI Egg PtdCho ... h incubation in Eagle’s mini-mum essential medium, the first 2 h without fetal bovine serum and < /b> the rest with < /b> 10% fetal bovine serum, cell viability was estimated by < /b> the MTT assay (absorbance...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... wereremoved at the time indicated and < /b> the reaction was stopped by < /b> the addition of phenylmethanesulfonyl fluoride, followed by < /b> boiling in the sample buffer. The samples obtained were subjected to quantitativeSDS ... (Fig. 4B, curve 1). If actin heated at 60 °Cwassubjected to trypsinolysis under the same conditions, the band of actin disappeared in the first 1 5–3 0 s (Fig. 4B, curve2) and < /b> a number of faint bands ... wereobtained by < /b> Benndorf et al. [13], but only with < /b> the monomeric unphosphorylated form of HSP25. Theseresults were obtained with < /b> HSP25 purified from avian or human < /b> tissues. In both cases the...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... availability in B. subtilis [1,9]. The feedback-inhibited form of GSbinds tightly to TnrA, preventing its binding to DNA, with < /b> the most effective feedback inhibitors of GS beingglutamine and < /b> ... tobe regulated by < /b> complex formation < /b> with < /b> feedback-inhibited GS, and < /b> in the absence of a nitrogen sourceTnrA is eliminated from the cells by < /b> proteolysis[9,10,15]. The findings in the present study ... interaction)< /b> ltnrA binds to glnK by < /b> pull down (View interaction)< /b> ltnrA binds to GS by < /b> pull down (View interaction)< /b> lGS physically interacts with < /b> tnrA by < /b> anti bait coimmunoprecipitation (View interaction)< /b> lglnK...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... responsible for the actual membranefusion step. However, the nature of the interaction < /b> ofviral fusion proteins with < /b> membranes < /b> and < /b> the mechan-ism by < /b> which these proteins accelerate the formation< /b> of ... of HGV ⁄ GBV-C with < /b> model < /b> mem-branes, in order to understand the possible mode ofpenetration of HGV ⁄ GBV-C into the membrane cells.These synthetic peptides are characterized by < /b> the pres-ence ... interaction.< /b> Results The E2 peptides synthesized are amphiphilic becauseof the presence of hydrophobic and < /b> hydrophilic aminoacids in their composition which make them watersoluble and < /b> able to associate with < /b> model...
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