Báo cáo khoa học: Interaction with model membranes and pore formation by human stefin B – studying the native and prefibrillar states docx

... 2008 FEBS Interaction < /b> with < /b> model < /b> membranes < /b> and < /b> pore < /b> formation < /b> by < /b> human < /b> stefin < /b> B – studying the native and < /b> prefibrillar states Sabina Rabzelj 1, * , †, Gabriella Viero 2, * ,à , Ion Gutie ´ rrez-Aguirre 3 , ... MA. Pore < /b> formation < /b> of stefin < /b> B into lipid membranes < /b> S. Rabzelj...

Ngày tải lên: 30/03/2014, 04:20

... FABP 1B (ZF Fabp 1B, DQ062096), FABP2 (ZF Fabp2, AAH75970), FABP3 (ZF Fabp3, NP_694493), FABP7A (ZF Fabp7A, NP_571680) and < /b> FABP 7B (ZF Fabp 7B, AAQ92970) and < /b> human < /b> FABP1 (Hu FABP1, M10617), FABP2 ... FABP 1B; DQ062096), FABP2 (ZF FABP2; AAH75970), FABP3 (ZF FABP3; NP_694493), FABP7A (ZF FABP7A; NP_571680), FABP 7B (ZF FABP 7B; AAQ92970), and < /b> human < /b> FABPs...

Ngày tải lên: 07/03/2014, 06:20

... with < /b> riboflavin, the protein was cocrystallised with < /b> riboflavin, and < /b> the crystals were subsequently soaked with < /b> riboflavin. Cocrystallization experiments with < /b> the substrate analogue NORAP a nd the product ... organisms studied [ 6–8 ,11], the enzyme from S. pombe binds riboflavin with < /b> relatively high affinity. This is believed to be due to a p...

Ngày tải lên: 30/03/2014, 15:20

... co-activators [34,35].TORCswereshowntobindtotheb-ZIPdomainof CREB, the binding being i ndependent of the Ser13 3– phosphorylation of C REB, and < /b> a ctivate CREB-mediated transcription. On the other hand, SIK1 was shown ... CAGGTCTG; the Bsp120I site is indicated by < /b> the underlined region). The cDNA fragments for the N -terminal regions and < /b> t he C-terminal re...

Ngày tải lên: 30/03/2014, 15:20

... that the membrane might be destabilized simply by < /b> surface interactions. However, the permeabilization by < /b> stefin < /b> B prefibrillar oligomers of vesicles made of acidic phospholipids resembles pore < /b> formation < /b> ... human < /b> stefin < /b> preparations. Cell viability was measured by < /b> the MTS test. Cells were exposed overnight to native stefin < /b>...

Ngày tải lên: 07/03/2014, 17:20

... at the membrane/water interface deepening the side-chains of hydrophobic residues at the tips of the loops 1–3 down to the boundary between the glycerol and < /b> acyl regions of the bilayer; (c) the ... viscotoxin and < /b> delta-haemo- lysin [37,38]. The data from this suggest that the modes of CTII interaction < /b> with < /b> the membranes < /b> can be describ...

Ngày tải lên: 17/03/2014, 03:20

... follows: bands in the regions 169 6–1 680 cm )1 and < /b> 167 0– 1660 cm )1 , b- turn; band at 1672 ± 2 cm )1 , antiparallel b- sheet; band at 1651 ± 3 cm )1 , a-helix; band at 1640 ± 2cm )1 , random coil; bands ... PtdIns 3-P NI – – Fatty acids Myristic acid 1.0 – – Palmitic acid 0.75 – – Stearic acid 2.0 – – Lipoproteins and < /b> plasma proteins Human...

Ngày tải lên: 23/03/2014, 21:20

... were removed at the time indicated and < /b> the reaction was stopped by < /b> the addition of phenylmethanesulfonyl fluoride, followed by < /b> boiling in the sample buffer. The samples obtained were subjected to quantitative SDS ... (Fig. 4B, curve 1). If actin heated at 60 °Cwas subjected to trypsinolysis under the same conditions, the band of actin disappeared in the first...

Ngày tải lên: 20/02/2014, 23:20

... availability in B. subtilis [1,9]. The feedback-inhibited form of GS binds tightly to TnrA, preventing its binding to DNA, with < /b> the most effective feedback inhibitors of GS being glutamine and < /b> ... to be regulated by < /b> complex formation < /b> with < /b> feedback- inhibited GS, and < /b> in the absence of a nitrogen source TnrA is eliminated from the cells by <...

Ngày tải lên: 06/03/2014, 00:21

... responsible for the actual membrane fusion step. However, the nature of the interaction < /b> of viral fusion proteins with < /b> membranes < /b> and < /b> the mechan- ism by < /b> which these proteins accelerate the formation < /b> of ... of HGV ⁄ GBV-C with < /b> model < /b> mem- branes, in order to understand the possible mode of penetration of HGV ⁄ GBV-C into the me...

Ngày tải lên: 07/03/2014, 17:20