Báo cáo khoa học: Engineering triterpene production in Saccharomyces cerevisiae – b-amyrin synthase from Artemisia annua docx

... Engineering triterpene production in Saccharomyces cerevisiae – b-amyrin synthase from Artemisia annua James Kirby 1 , Dante W. Romanini 2 , Eric M. Paradise 1,3 and Jay D. Keasling 1,3,4,5 1 ... synthase gene from A. annua and its expression in S. cerevisiae. Our findings on engineering overproduction of b-amyrin in S. cerevisiae should be relevant t...

Ngày tải lên: 30/03/2014, 04:20

... channels by binding in deep crevices near the pore but intracellularly of the gate. J Gen Physiol, 133, 18 9–2 03. 70 Metelkin E, Demin O, Kovacs Z & Chinopoulos C (2009) Modeling of ATP–ADP steady-state ... dechorionated in modified antiformin solu- tion (1% hypochlorite from bleach, 60 mm NaCO 3 , 0.4 m NaOH) for 30 min, and this was followed by a rinse in 1% sodum thiosulfate...

Ngày tải lên: 29/03/2014, 00:20

... by these inhibitors. In addition, the inhibitor mode was also determined. The data collected at varied shikimate (or NADP) and inhibitor concentrations yielded a ser- ies of intersecting lines when ... AroE from several bacteria such as E. coli, Methanococcus jannaschii, and H. in uenzae, and YdiB from E. coli, including structures of enzyme–cofactor complexes, have been published [2...

Ngày tải lên: 19/02/2014, 05:20

... substitution of glycine by alanine and proline, respectively, or of proline by glycine (Table 1). Each individual mutation was verified by DNA sequencing using the dideoxynucleotide chain-termination method ... point mutations were initiated aiming at the substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the...

Ngày tải lên: 21/02/2014, 03:20

... discriminating correct parse trees from incorrect ones, while at the same time maintaining the tractability of learning. Some work in machine learning has taken an al- ternative approach to defining ... tags. 185 occur in the training set in order to be included ex- plicitly in the vocabulary. A frequency threshold of 200 resulted in a vocabulary of 508 tag-word pairs (including ta...

Ngày tải lên: 08/03/2014, 04:22

... the residues involved in binding of the [Fe–S] clus- ter. The canonical binding motif for the [4Fe–4S] 2+ cluster CXXCXXCX was found in many quinolinate synthases from bacteria (including E. coli ... quinolinate synthase, in particular for NadA proteins that do not contain a canonical binding motif for a [4Fe–4S] 2+ cluster; and (b) the identifica- tion of the residues involved in t...

Ngày tải lên: 30/03/2014, 02:20

... in l-asparaginase- induced antithrombin III deficiency. Blood 83, 38 6–3 91. 9 Moola ZB, Scawen MD, Atkinson T & Nicholls DJ (1994) Erwinia chrysanthemi l-asparaginase: epitope mapping and production ... (1999) Increasing protein stability by altering long- range columbic interactions. Protein Sci 8, 184 3–1 849. 44 Loladze VV, Ibarra-Molero B, Sanchez-Ruiz JM & Makhatadze GI (...

Ngày tải lên: 30/03/2014, 02:20

... followed their own definitions of different scoring values, such as excluding certain scores (see examples in table 1). They find the def- initions incorrect. If the veterinarian strictly follows ... extract as much information as possible from each con- text of interest, allowing in this case for a long interaction period between each interviewed cattle veterinarian work- ing with a he...

Ngày tải lên: 25/10/2012, 10:45

... GS staining (B3), and 4¢,6-diamidino-2-phenylindole (DAPI) staining (B4), respectively. (B5-8) High magnification images of the boxed area in (B4) showing RPE65c staining (B5), GS staining (B6), ... atRE, all-trans retinyl ester; atROL, all-trans retinol; CRALBP, cellular retinaldehyde-binding protein; GFP, green fluorescence protein; GS, glutamine synthetase; LRAT, lecithin retinol acyltrans...

Ngày tải lên: 14/02/2014, 14:20

... protein kinase; MCF, macrophare chemotactic factor; MCIP, modulatory calcineurin-interact- ing protein; MDCK, Madin Darby canine kidney; MEF, mouse embryonic fibroblast; MEK, MAP kinase/ERK kinase; ... phosphofructokinase; PFL, pyruvate formate- lyase; Pi, inorganic phosphate; PI3K, phosphatidylinositol 3-kinase; PIP, phosphatidylinositol 4,5-bisphosphate; PKA, protein kinase A; PKC, protein k...

Ngày tải lên: 14/02/2014, 14:20