Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt

... N2-carboxyethyl-2¢-deoxyguanosine, a DNA glycation marker, in kidneys and aortas of diabetic and uremic patients. Kidney Int69, 388–392. DNA advanced glycation end-products in cells V. Breyer et al.924 ... al. DNA advanced glycation end-products in cells FEBS Journal 275 (2008) 914–925 ª 2008 The Authors Journal compilation ª 2008 FEBS 925 Analysis and biological relevance of advanced glycation end-products ... accumulate in genomic DNA of nongrowing cells, HSC-T6 cells werekept in minimal medium containing 1.5% fetal bovineserum for 2 weeks. The composition of the mediumwas adjusted to minimize growth and...

Tài liệu Báo cáo khoa học: "Analysis and Repair of Name Tagger Errors" pptx

... understanding of the benefits of joint inference is a prerequisite for further improvements in name tagging perform-ance. After summarizing some prior work in this area, describing our baseline ... part of common words. 3.1 Identification and Classification Errors We begin our error analysis with an investigation of the English and Chinese baseline taggers, de-composing the errors into ... reranking in the extraction pipeline, and analyze the results in terms of dif-ferent types of identification and classification errors. 2 Prior Work Some recent work has incorporated global infor-mation...

Báo cáo khoa học: "Analysis and Synthesis of the Distribution of Consonants over Languages: A Complex Network Approach" pptx

... of Figure 3(b) comprises of the rest of the consonants. The point marked as x in boththe ﬁgures indicates the breakpoint. Each of theregime in both Figure 3(a) and (b) exhibit a powerlaw of ... proper perception in a noisy environment. In fact the organization of the vowel inventoriesacross languages has been satisfactorily explained in terms of the single principle of maximal percep-tual ... 2000).Modeling of complex systems as networks hasproved to be a comprehensive and emerging way of capturing the underlying generating mecha-nism of such systems (for a review on complexnetworks and...

Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... Parameters of SP and SP analogues.Ó FEBS 2003 Eﬀects of b-amino acid insertion in Substance P (Eur. J. Biochem. 270) 949Structural and biological effects of a b2-orb3-amino acid insertion in a ... out at intervals of 30° for each torsion angle/, h,andw and were subsequently reﬁned by varying /, wangles in intervals of 10° and setting h angle to ) 60 ± 10° and 60 ± 10°.Each/, h,andw-value ... in blue, oxygens in red, hydrogens in grey. Carbon atoms of Pro are coloured in cyan, those of other amino acids in green. Theside chain methyl carbon of b-HAla and thepro R methyl carbon of...

Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx

... replicating TTV DNA from the input; and the otherlabelled with digoxigenin (DIG) additionally documentsthe susceptibility of the input DNA to DpnI. In cells transfected with linTTV, the input DNA ... replicating TTV DNA in the presentstudy was not produced with the help of endogenousTTV. Interestingly, the amount of replicatingTTV DNA was much lower in 293 than in 293T cells, suggesting that ... 293 and 293T cells in Fig. 3A,C). The non-transfected cells and the backbone plasmid-transfected cells were always negative for TTV DNA. In Southern analysis, the input DNA served as an internal...

Tài liệu Báo cáo khoa học: Diego and friends play again Old planar cell polarity players in new positions doc

... proteins; indeed, the In staininglargely overlaps with that of Fz. At present, it is notclear how In is recruited to the proximal side of thewing cells. One tempting possibility is that In isrecruited ... Eachommatidium consists of 20 cells including eight photo-receptor cells and 12 supporting cells. Sectioning of theadult eye reveals that the ommatidia are chiral struc-tures as photoreceptor cells aquire ... cellboundary in the wing. Consistent with the proteindistribution in the eye, for fz and dsh there are geneticrequirements in R3 [32], for stbm and pk in R4 [5,41], and for fmi in both R3 and R4...

Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of ﬁve novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx

... comparison of bindingafﬁnities of the inhibitors under study.The ﬁtting of the binding isotherms of all ﬁve com-pounds with a binding model assuming identical and independent binding sites ... main chain nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitro-gen of Asn114¢ of the other subunit. The contacts of the ribityl chain to ... range. The analysis of the struc-tures demonstrated the speciﬁc features of the binding of different inhibi-tors. The comparison of the structures and binding modes of ﬁve differentinhibitors...

Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx

... serine ⁄ threonine kinase domain in both proteins isencoded by two exons equivalent to kinase subdomainsX and XI [22]. In addition, the GS box and L45 loop of Cg-BMPR1, as well as the C-terminal ... resulting in the produc-tion of dorsalized phenotypes. Injection of relativelyhigh concentrations of mRNA encoding the C2domain, however, resulted in embryos with a combina-tion of features of ... amputa-ted of its highly variable C-terminal domain after theterminal conserved arginine of the cytoplasmic serine ⁄threonine kinase domain. Sequences for phylogenetic rela-tionships of the extracellular...

Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf

... doi:10.1046/j.1432-1033.2002.03129.xLeu-enkephalin from the enkephalin-containing peptides[22], and the speciﬁc TOP inhibition increased Met-enke-phalin antinociception in rodents [23]. TOP and neurolysinare able to hydrolyze the biologically ... the involvement of TOP in sorting and hydrolysis of the peptides generated by proteasomes [30–32].A detailed analysis of the substrate speciﬁcity of neu-rolysin and TOP was reported using internally ... TOP and neurolysin inhibitors [24] and a highlyspeciﬁc neurolysin inhibitor potentiated the neurotensin-induced antinociception of mice in the hot plate test whenadministrated intracerebroventricularly...

Báo cáo khoa học: A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus ppt

... Electrophoretic analysis was car-ried out using 5% SDS ⁄ PAGE to facilitate detection of high molecular mass proteins.MSPeptide mass ﬁngerprinting and LIFT-TOF ⁄ TOF MS analysis of trypsin-digested ... containing pes1E2was trans-formed into Escherichia coli strain DH5a. After conﬁrmat-ory DNA sequence analysis, expression of pes1E2wasinduced and recombinant Pes1E2puriﬁed [44]. RecombinantPes1TEA(Fig. ... resuspended in 500 lL of TM, and 50 lLaliquots were mixed with 10 mL of AMM (minus uracil and uridine) containing 1 m sorbitol as osmotic stabilizerplus 2% (w ⁄ v) molten agar, and then poured onto min-imal...

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