Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf

... 27 1–2 80 ª 2007 The Authors Journal compilation ª 2007 FEBS Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior ... protein engineering of this enzyme. In the present study, the prolidase- coding gene, pepQ, was isolated from Lactococcus lactis NR...

Ngày tải lên: 30/03/2014, 04:20

... pathogens, the cell wall is involved in the interaction with the host cells and in virulence. The biogenesis of the extracellular matrix is a fascinating aspect of yeast m orphogenesis. The elucidation ... ollowed by the release of the cleaved product and stabilization of the carbon cation by the catalytic nucleophile. The new reducing end is then tra...

Ngày tải lên: 16/03/2014, 18:20

... up in the gel (Fig. 1B, lane 6). A subsequent incubation of the gel in DPI resulted in inhibition of the lower band only (Fig. 1B, lane 8). On the basis of the size of multimers of ferritin used ... oligomer of the small form. The different in uences of DPI on the two forms could be caused by its failure to access the flavin cofactor in the large fo...

Ngày tải lên: 07/03/2014, 09:20

... f isocitrate, and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23]. In addition, the alkylation of the adjacent M258 inactivated the IDH from A. vinelandii [26]. These ... ere not identified in the 5¢-flanking region of Cl-idh. No open reading frames were found in the immediate vicinity of the gene. The deduced amino-acid sequ...

Ngày tải lên: 08/03/2014, 10:20

... amino acids in the Triton-bound con- formation of SCP-2. The amino acids lining the cavit- ies in the E. lagascae and A. thaliana Tr-SCP-2 models and in the structure of the SCP-2-like domain of human ... mode of ergosterol in the structural model of A. thaliana SCP-2. The binding mode in (A) has higher fitness than the binding mode in (B). The pol...

Ngày tải lên: 16/03/2014, 12:20

... [1]. The variety of identified substrates indicates a critical role for PS in cell metabolism involving controlled cleavage of protein transmembrane domains and signal transduction. The role of ... trans- membrane domains six and seven to generate heterodimers of N-terminal fragments (NTF) and C-terminal fragments (CTF) [7]. Many binding partners for the PS proteins have...

Ngày tải lên: 16/03/2014, 16:20

... that, in PhoACY, the mutation of one zinc ligand allows the local loosening of Zn 2+ and the intervention of another Zn 2+ in the metal- binding pocket, resulting in the incorrect coordination of ... shown by a pink sphere. (B) Speculated zinc-binding domain of PhoACY. Zinc ion is shown by a pink sphere. The residues related to zinc binding are shown by...

Ngày tải lên: 23/03/2014, 07:20

... nonsteroidal agonists bind exclusively to the EcR ligand-binding domain (LBD), although the presence of USP increases ligand-binding affinity [1 2–1 5]. The diversity of ligand-responsive characteristics seen ... stretch of 3 5–3 7 amino acids that lie just to the N-terminal side of the DBD (Fig. 1C). The EcRA isoforms from the two species share a few similar motifs...

Ngày tải lên: 18/02/2014, 08:20

... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linked galactosyl residues, and a terminal-linked N-acetylglu- cosaminosyl ... deprotonated [M-H] – ions. The source of the ions was as indicated in the structure shown in the inset. The m ⁄ z 917.4 and 797.4 are due to sodiated and d...

Ngày tải lên: 07/03/2014, 05:20

... cellulose-binding domain (CBD) [ 7–1 0]. As the ini- tial step of the reaction, they are adsorbed on the surface of crystalline cellulose via the CBD, then glu- cosidic linkages are hydrolyzed by the ... adsorption of Cel7A after incubation for 120 min as representative results of four time points (120, 180, 240, and 320 min). The lines indicate the fitting of th...

Ngày tải lên: 23/03/2014, 09:21