... Putative prion protein from Fugu (Takifugu rubripes) Barbara Christen, Kurt Wu¨thrich and Simone HornemannInstitute of Molecular Biology and Biophysics, ETH Zurich, Switzerland Prion ... At the outsetof this study, we tried to express and purify putative globular domains of Fugu (Takifugu rubripes) PrP1 (aa298–423), Fugu PrP2 (aa 215–404), zebrafish (D. rerio)PrP1 (aa 389–581) ... with PrPs from mammals, birds, reptiles and amphibians.AbbreviationsGPI, glycosylphosphatidylinositol; IPTG, isopropyl thio-b-D-galactoside; mPrP, mouse prion protein; PrP, prion protein; tr1-PrP,...