Báo cáo khoa học: Putative prion protein from Fugu (Takifugu rubripes) ppt

... Putative prion protein from Fugu (Takifugu rubripes) Barbara Christen, Kurt Wu ¨ thrich and Simone Hornemann Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland Prion ... At the outset of this study, we tried to express and purify putative globular domains of Fugu (Takifugu rubripes) PrP1 (aa 298–423), Fugu PrP2 (aa 215–404), zebrafish (D. rerio...

Ngày tải lên: 30/03/2014, 04:20

... of Rieske proteins. Experimental procedures Bioinformatic tools Protein sequences were obtained from Swiss-Prot pro- tein database (http://www.expasy.org/sprot); (a) bacterial Rieske proteins: ... 15475–15483. Rieske protein from Paracoccus denitrificans J. Bachmann et al. 4830 FEBS Journal 273 (2006) 4817–4830 ª 2006 The Authors Journal compilation ª 2006 FEBS The Rieske protein fr...

Ngày tải lên: 07/03/2014, 11:20

... and photosys- tems I and II from hydrogen peroxide-mediated oxidative damage. Abbreviations Dps, DNA-binding proteins from starved cells; Dps-Te, DNA-binding protein from starved cells of Thermosynechococcus ... September 2006) doi:10.1111/j.1742-4658.2006.05490.x DNA-binding proteins from starved cells (Dps proteins) protect bacteria primarily from oxidative damage. They are comp...

Ngày tải lên: 16/03/2014, 12:20

... inferred from raw text. In addition, the number of iterations needed to reach a good grammar can be reduced; in extreme cases, a good solution is found from parsed text but not from raw text. ... ( (from SF0) (to San Francisco))))).) GR (Tell ((me (((about the) public) transportation)) ( (from SF0) ((to San) (Francisco .))))) GB ((Tell (me (about (((the public) transportation)...

Ngày tải lên: 20/02/2014, 21:20

... tree T dep being created, one par- ticular node s p h, from T p h, (the root of the phrase subtree to be processed), and node p dep from T dep (the future parent of the subtree being processed). The ... and (ii) a procedure as- signing analytical functions to the nodes of the dependency tree. Apart from an outline of the annota- tion schemes and a deeper insight into these proce...

Ngày tải lên: 22/02/2014, 02:20

... initial experiment used a database of over 100 000 proteins, including protein domain sequences of known structure from SCOP as well as protein sequences from SWISS- PROT. Because visualizing such a ... [1], yields good performance from rankprop but not from psi-blast. This protein is a member of the PYP-like sensor domain SCOP superfamily [16], which in our experiment contains...

Ngày tải lên: 07/03/2014, 21:20

... descrip- tions of images from computer vision de- tections. By leveraging syntactically in- formed word co-occurrence statistics, the generator filters and constrains the noisy detections output from a vision ... natural- sounding descriptions from computer vision de- tections. This commonality is our starting point: We aim to design a system capable of producing natural-sounding descrip...

Ngày tải lên: 31/03/2014, 21:20

... bacterial purified protein The GST–s-DAPK-1-transformed BL21 cells were induced with arabinose for 3 h and lysed with 0.2% Triton in NaCl ⁄ P i . The GST–s-DAPK-1 protein was then extracted from the lysate ... Authors Journal compilation ª 2008 FEBS An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane bleb...

Ngày tải lên: 18/02/2014, 17:20

... Reagent were purchased from Invitrogen (Carlsbad, CA). pGEM-T vector was obtained from Promega Corporation (Madison, WI, USA). The Cdk5 inhibitor, roscovitine, was obtained from Biomol Research ... Biochem. 269) Ó FEBS 2002 the supernatants were determined using BCA protein reagent. An equal amount of total protein (20 lgof protein per lane) was resolved on a 4–20% SDS-polyacryl-...

Ngày tải lên: 23/03/2014, 21:21

... component of the amyloid plaques, the amyloid-b protein (Ab), is a polypeptide of 39–43 amino-acid residues, which is derived from a larger amyloid-b protein precursor (APP) [1–4]. Ab can poly- merize ... Mechanisms of Ab aggregation (Eur. J. Biochem. 270) 4293 b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing...

Ngày tải lên: 31/03/2014, 07:20