... myokinase, enolase, lactate dehydrogenase, andglutamate dehydrogenase from Oriental Yeast, Osaka,Japan.Other analytical methods The N-terminal amino-acid sequence of the enzyme wasdetermined ... Toyobo (Osaka, Japan), and New EnglandBiolabs (Beverly, MA, USA). All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture ... use ammonia as a substrate and was distinctfrom alanine dehydrogenase [18] and N-methylalaninedehydrogenase found by Lin & Wagner [17]. Lysineand ornithine were inert as an amine substrate;...