Báo cáo khoa học: Monitoring the prevention of amyloid ﬁbril

Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... study by showing the direct interaction of Utp25p with SSU pro- cessome subunits, and the analysis of the 35S pre-rRNA cleavage reactions that are affected by the depletion of Utp25p. Furthermore, ... UTP-B and UTP-C [18,19]. t-UTP complex binds very early during transcription of the pre-rRNA, followed by the UTP-B complex, U3 snoRNP and the Mpp10p complex,...

Ngày tải lên : 22/03/2014, 21:21

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Báo cáo khoa học: Monitoring ligand-mediated nuclear receptor–coregulator interactions by noncovalent mass spectrometry pptx

... different species on the ESI mass spectrum reﬂect the actual distribution of these species in solution. The reproducibility of the determination of the relative p roportions of the different species ... bind to the HD in the absence of any ligand, and (b) ESI-MS data allow one to directly monitor the effect of ligand binding on the association/ dissocia...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Structural ﬂexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf

... of the atoms. The eigenvalues reflect the magnitude of the fluctuation along the respective EVs. Structural superpositions and other conformational analyses were performed using lsq- man [56] and ... represented, the former by PAH_1 and FPAH and the latter by PEP and PAH_2. This appears to be the first time that an enolase-PAH complex has crys- tallised in a non...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: Monitoring the prevention of amyloid ﬁbril formation by a-crystallin Temperature dependence and the nature of the aggregating species pdf

... Journal compilation ª 2007 FEBS 6305 Monitoring the prevention of amyloid ﬁbril formation by a-crystallin Temperature dependence and the nature of the aggregating species Agata Rekas 1,2 , Lucy Jankova 3 , ... detail, two aspects of a-crystallin s ﬁbril- suppressing ability: (a) its temperature dependence, and (b) the nature of the agg...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

... implemented to help explain their importance to the speciﬁcity and catalytic efﬁciency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an unreacted molecule of the mechanism-based ... solved the crystal structure of this GH5 enzyme in the presence of two different mechanism- based inhibitors, thereby revealing the close network o...

Ngày tải lên : 15/03/2014, 23:20

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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf

... is often characterized by an increase in both the size and the number of adipocytes. Previous data have shown that adipose tissues play crucial roles in the development of obesity, and that the ... promote the formation of lipid droplets. However, the detailed roles of human CIDEC in the differentiation of human adipocytes remain unknown. In the present stu...

Ngày tải lên : 23/03/2014, 03:20

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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... peptide was identified by the almost complete series of y-fragments and a long series of b-fragments (Fig. 4). From the difference of the precursor mass to the y-28 fragment and from the b1 fragment, ... labeling of P40 within the gel by the CAF reagent occurred in a very efficient way and allowed the identification of the new N-terminus after cleavage of...

Ngày tải lên : 19/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... high level of the polyunsaturated fatty acid linoleic acid [40,41]. They are prime target of ROS due to their location near to the site of ROS production [42] and could be the source of carbonyl adducts. ... at )80 °C for further determinations of protease activity and the level of carboxymethylated protein. Protein was assayed by the Bradford method. To estimate...

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... calculate the inﬂection point of the transition and hence the T m [14]. Assessment of the polymerization of wild-type and Ser52Arg neuroserpin Polymerization of wild-type, Ser49Pro and Ser52Arg ... 24 h. Assessment of the conformation of Ser52Arg neuroserpin The most likely cause for the inactivity of Ser52Arg neuroserpin and inaccessibility of the reac...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc

... releases the activator domain in the cytosol, which can then traffic to the nucleus to initiate the transcription of the green fluorescence protein (GFP) gene by binding to the 5Gal-E1b domain of the ... (kindly provided by F. Reinhard) and was cloned into pIRESpuro2. We thereby removed the N-terminal flag sequence. The last three mutations did not contain the VRSQ-s...

Ngày tải lên : 08/03/2014, 08:20

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