Báo cáo khoa học: Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium pptx

... (2007) 5727–5736 ª 2007 The Authors Journal compilation ª 2007 FEBS Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium Takuya ... 2007) doi:10.1111/j. 1742 -4658.2007.06092.x The basidiomycete Phanerochaete chrysosporium produces xyloglucanase Xgh74B, which has the glycoside hydrola...

Ngày tải lên: 30/03/2014, 03:20

... of family 13 (reviewedin[38]). The exo-acting glucoamylase s imilarly to the a-glucosid- ases catalyses the releases of glucose from the nonreducing ends of substrates, but with inversion of the ... for the yeast a-glucosidase and oligo-1,6-glucosidase of glycoside of hydrolase family 13 when compared to that of glucoamylase of glycoside hydrolase family 15 or of a...

Ngày tải lên: 23/03/2014, 21:21

... site lysine of the protein. In the second step, the AMP i s transferred from the protein to the 5¢ phosphate group of the nick on the DNA t o form DNA ±adenylate . In t he third step, the enzyme ... ligase (Lig Tk )froma hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. The enzyme is the only characterized ATP-depend- ent DNA ligase from a hyperthermophile,...

Ngày tải lên: 17/03/2014, 11:20

... nitrogen and the Asp146 carboxylate, which could weaken the Asp146–Asn91 interaction that decreases the Lewis acidity of the iron(II) ion and thereby the electrophilicity of the activated substrate molecule ... min), but even at the lowest pH the enzyme was stable over the time range measured. Although the k cat values have rather small errors, the plot of K M vs. pH s...

Ngày tải lên: 23/03/2014, 13:20

... lymphoma [7–9]. The gene HP1287 from H. pylori shows 33% sequence identity to the tenA gene from Bacillus subtilis. Furthermore, the tenA gene in B. subtilis is part of the thiazole biosynthetic operon, ... 33% identity and 51% similarity to the TenA protein from B. subtilis. The 3D structure of the monomer is quite similar to that of the other members of the TenA fam...

Ngày tải lên: 16/03/2014, 00:20

... response, thereby minimizing damage to the host induced by response to a pathogen or by the self-immune system. IL-10 inhibits the activation of macrophages/monocytes, thereby inhibiting cytokine synthesis, ... their positions. The arrowheads depict the residues important for the structural core of the IL-10 gene. The underlined amino acid residues are the signal sequenc...

Ngày tải lên: 20/02/2014, 02:21

... (TM0437), from the hyperther- mophilic bacterium Thermotoga maritima was functionally produced in Escherichia coli and purified to homogeneity. PelB belongs to family 28 of the glycoside hydrolases, ... end towards the N-terminus of the enzyme [31], this implies that PelB probably contains four sub- sites, from )1 to +3. Discussion The pectinolytic hydrolase PelB from th...

Ngày tải lên: 20/02/2014, 03:20

... enhancement mediated by introns. Indeed, the presence of the PAP Gld2 on the messen- ger, targeted by a protein of the Rbm9 family, may allow the polyadenylation of the messenger regulated by Rbm9, hence ... XGld2: the XGld2 ORF from pCRII XGld2 was inserted into the Cla I-KpnI sites of the pBSKS+ vector. The XGld2 ORF from pKS XGld2 was then inserted in frame w...

Ngày tải lên: 07/03/2014, 05:20

... First, we tested whether these messages are translatable. In the second step, we characterized their coupling to different signaling pathways and their modu- latory role on the CRFR1a activity. Materials ... terminator. These constructs were also assembled in three steps. In the first PCRweamplifiedexons1–4byprimersE3andE24 (5¢-CTCCTCATTGAGGATCTCCT-3¢). The second PCR amplified the cry...

Ngày tải lên: 07/03/2014, 15:20

... information to the thing • Identifying: identifies the thing among other possible referents • Quantifying: determines the quantity or amount of the thing. The main modifications from the existing ... input structure are the introduction of the re/-set feature and the update of the usage of the possessor feature. For both of these features, the main re- quire...

Ngày tải lên: 08/03/2014, 06:20