Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx
Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx
... centrifugation, and analyzed by SDS ⁄ PAGE and protein staining with Coomassie Brilliant Blue. The protein ladder was run on the same gel (lane 5). Pancreatic endoplasmic reticulum chaperone network A. ... major ER-lumi- nal heat shock protein (Hsp) 70, IgG heavy chain-binding protein (BiP), at least one of which also involves the second ER-luminal Hsp70, gluco...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx
... small heat shock protein with molecular mass 25 kDa (hsp2 5) with actin. Eur J Biochem 270, 892–901. 22 Brophy CM, Lamb S & Graham A (199 9) The small heat shock- related protein- 20 is an actin-associated ... before F-actin thermal denaturation (at 30–35 °C) (A) and after F-actin denaturation (at 70 °C) (B). Condi- tions were the same as in Fig. 1A. Each plot...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf
... 5¢-CACGTACAAAGAGAACGTCGACGACG-3¢ 5¢-CGTCGTCGACGTTCTCTTTGTACGTG-3¢ R11 4A 5¢-GAGAATTTCGAGCACGATACAGACTCCC-3¢ 5¢-GGGAGTCTGTATCGTGCTCGAAATTCTC-3¢ Y116D 5¢-CGACGACGAGACAGACTCCCAGAACATGTC-3¢ 5¢-GACATGTTCTGGGAGTCTGTCTCGTCGTCG-3¢ Y. ... H, The ´ riault JR & Landry J (200 5) Structural instability caused by a mutation at a conserved arginine in the a- crystallin domain of Chinese h...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein doc
... PDH, and induces aggregation of the T -protein [3]. An analogous bifunctional protein in E. coli, known as the P -protein, contains CM and prephenate dehydratase (PDT), and catalyzes the transformation ... Phe-binding domain, T -protein fragment analysis indicated that tyrosine binding and feedback inhibition could not be attributed to a structural domain that was separate from...
Ngày tải lên: 31/03/2014, 07:20
Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf
... chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked by a disulfide ... using the following protein standards (Amersham): RNase (13.7 kDa), chymotrypsino- gen (25 kDa), ovalbumin (43 kDa), BSA (67 kDa) and Blue Dextran (2000 kDa)....
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... ferritin (DAKO); 5F6, a monoclonal antibody to oxidatively modified proteins containing the aldehyde adduct acrolein (a gift from K. Uchida 8 , Nagoya University, Nagoya, Japan). Rabbit anti-mouse and ... and stored at )7 0 °C for further analysis. From each sample, total RNA was isolated and DNAse treated using the RNeasy kit according to the manufacture specifications (Qiagen, Vict...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... particular, most of the essential features of the catalytic site (i.e. a catalytic triad of Ser-His-Glu, an oxyanion hole, a p-cation-binding site, and an acyl-binding pocket) are the same in AChE and ... BuChE, as indicated. In each panel, the solid circles indicate the measured k cat values, the solid squares indicate the measured bk cat values, and the lines are th...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx
... 5¢-GTTCTCG AAGGTGTG TGCGAGCGAGTCGTCCGCGAAG-3¢ (C44S), 5¢-GTTGCGTGCGAGTCGAGCGCGAAGAC GGGCATG-3¢ (C47S), 5¢-CTG GACTACGAGTCGAG CAATGTGCTGGTTGCG-3¢ (C92S), 5¢-CAGTCGCC GGACATCAGCCAGGGTCTGGGCAAC-3¢ (C106S), 5¢-GGCCTGGCGCGCCGCAGCCTTGTGCAGCTCG CG-3¢ ... Thermus aquaticus (Taq) poly- merase was from Promega and Pyrococcus furiosus (Pfu) polymerase was from Stratagene (La Jolla, CA, USA). Leishmania...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
... part of the GAPDH (a PTS-1 protein) , ALD (a PTS-2 protein) and TIM (an I-PTS protein) is already released from the cells at concentrations of digitonin that leave the glycosomal membrane intact. ... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S. cerevisiae that both the N-terminal and the C-terminal dom...
Ngày tải lên: 23/03/2014, 17:21
Báo cáo khoa học: First evidence of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin models ppt
... of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin models Francesca d’Acunzo and Carlo Galli Dipartimento di Chimica, Universita ` ‘La Sapienza’, Roma, Italy; ... can be oxidatively converted into a reso- nance-stabilized phenoxy radical, performs as a mediator in the laccase-catalyzed oxidation of a nonphenolic substrate (4-methoxybenzyl alc...
Ngày tải lên: 23/03/2014, 21:20