... neither autophagy nor metabolismcould be the < /b> primary cause of < /b> the < /b> loss of < /b> fermentativecapacity. Rather, a subtle interplay between the < /b> twowas observed from the < /b> beginning. The < /b> diversity of < /b> ... understand the < /b> patterns of< /b> enzyme capacities over time, we should rather considerthem as the < /b> result of < /b> a balance between protein synthe-sis and < /b> breakdown from the < /b> beginning. The < /b> differencesbetween ... contained5 lL of < /b> SYBR Green PCR Core Kit (Bioke, Leiden, The< /b> Netherlands), 3 pmol of < /b> each primer (Isogen, De Meern, The < /b> Netherlands or Biolegio, Nijmegen, The < /b> Netherlands) and < /b> 3 lL of < /b> cDNA template...