Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot

... of muscarinic acetylcholine receptor -induced Ins(1,4,5)P 3 increases in PC12h cells, we utilized an Ins(1,4,5)P 3 imaging system based on fluorescence resonance energy trans- fer and using green ... containing CCh, which induced a second calcium increase and a further increase in Ins(1,4,5)P 3 (Fig. 3Aa). Both of these calcium increases were accompanied by increa...

Ngày tải lên: 30/03/2014, 03:20

... PBMC in rabbits was in parallel with the levels of interleukin-1b and interleukin-6 in the supernatants. The release of interleukin-1b and interleukin-6, nuclear translocation of NF-jB and its DNA ... of noradrenalin in the hypothalamus reduces the febrile response induced by prostaglandin E2, thyrotropin-re- leaseing hormone and b-endorphin. Neuropharmacology 24, 1039–104...

Ngày tải lên: 19/02/2014, 00:20

... was estimated from MDA measurements as shown in Fig. 1. At high Cu 2+ concentration (175 l M ),theMDA production decreases with increasing quercetin concentra- tion (Fig. 1A). It is worth noting ... Flavonoids and urate in copper -induced low-density lipoprotein oxidation (Eur. J. Biochem. 271) 1993 up to about 2 h. Finally, as shown in Fig. 2(C), slightly pro-oxidant concent...

Ngày tải lên: 23/03/2014, 12:20

... on the interaction between prealbumin, retinol-binding protein, and vitamin A. J Biol Chem 246, 44–49. 71 Bellovino D, Morimoto T, Tosetti F & Gaetani S (1996) Retinol binding protein and transthyretin ... Thyroxine -induced con- formational changes in prealbumin. Biochemistry 17, 5729–5733. 69 Kanai M, Raz A & Goodman DS (1968) Retinol-bind- ing protein: the transport protein...

Ngày tải lên: 07/03/2014, 10:20

... Scot- land. Minqing Hu and Bing Liu. 2004. Mining and sum- marizing customer reviews. In KDD’04: Proceed- ings of the Tenth ACM SIGKDD International Con- ference on Knowledge Discovery and Data Mining, pages ... match, and (iii) subset matching in which a span has to be contained in another span in order to be consid- ered as a match. 8 Agreement naturally increases as w...

Ngày tải lên: 16/03/2014, 23:20

... N-terminal segment [54]. Hexokinase-I is predominantly located in brain, kidney, retina and breast, whereas hexokinase-II is abundant in skeletal muscle and adipose tissue [8]. In tumor cells, ... citrate and l- lactate were determined by standard enzymatic assays [61]. Protein was determined by the biuret method using BSA as standard [62]. Determination of intracellular gl...

Ngày tải lên: 30/03/2014, 11:20

... catalyzed by GDP- L -fucose pyrophosphorylase. We report here the cloning and expression of murine L -fucokinase and GDP- L -fucose pyrophosphorylase. Murine L -fucokinase is expressed as two transcripts ... fucokinase in mammalian cells The two splice variants of the murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2- Tag present in the...

Ngày tải lên: 30/03/2014, 13:20

... an increase in [Ca 2+ ] i induced by extracellular calcium was again observed (Fig.7A).Furthermore,increasing[Ca 2+ ] o from 0–1.5 m M still significantly increased intracellular calcium in 30 cells from ... occurring polyamines, including spermine, spermidine and putrescine, are involved in the synthesis of nucleic acids and proteins in eukaryotic and prokaryotic cells. T...

Ngày tải lên: 31/03/2014, 01:20

... iron was removed by washing the cells with DMEM containing 50 l M DTPA and two more washings with DMEM alone. IVI induced cell injury was assessed by measuring leakage of lactate dehydrogenase ... IVI were added and changes in calcein -fluorescence were measured. Within the first 15 min of incubation with IVI, the LIP increased (i.e. baseline fluorescence decreased) between...

Ngày tải lên: 31/03/2014, 07:20

... FEBS protein in the two cell lines was determined as described in Materials and methods, and 81 lg of pro- tein was added to each well. The amount of S100A4 in the II-11b cells was 1.2% of that in the ... and TIMP-1. Previously, it has been shown that the reduced level of S100A4 in the II-11b cells compared with pHb-1 cells resulted in a large reduction of in viv...

Ngày tải lên: 18/02/2014, 11:20