Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
Báo cáo khoa học: Receptor- and calcium-dependent induced inositol 1,4,5-trisphosphate increases in PC12h cells as shown by fluorescence resonance energy transfer imaging pot
... of muscarinic acetylcholinereceptor -induced Ins(1,4,5)P3 increases in PC12h cells, we utilized anIns(1,4,5)P3 imaging system based on fluorescence resonance energy trans-fer and using green ... containing CCh, which induced a second calciumincrease and a further increase in Ins(1,4,5)P3(Fig. 3Aa). Both of these calcium increases wereaccompanied by increased Ins(1,4,5)P3, indicating ... (CFP–PHD and YFP–PHD, respectively) werecoexpressed in PC12h cells, and their interaction wasanalyzed by measuring changes in fluorescence inten-sity. Increases in cellular calcium and Ins(1,4,5)P3wereinduced...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... PBMC in rabbits was in parallel with thelevels of interleukin-1b and interleukin-6 in the supernatants. The releaseof interleukin-1b and interleukin-6, nuclear translocation of NF-jB and itsDNA ... ofnoradrenalin in the hypothalamus reduces the febrileresponse induced by prostaglandin E2, thyrotropin-re-leaseing hormone and b-endorphin. Neuropharmacology24, 1039–1042.C L. Su et al. SEC1 induces ... phenyl-methylsulfonyl fluoride and 1 lgÆmL)1each of antipain,leupeptin, aprotinin, and pepstatin A) and then incubatedfor 20 min on ice with occasional mixing. The nucleardebris was removed by centrifugation...
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Báo cáo khoa học: Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate potx
... was estimated from MDA measurements as shown in Fig. 1. At high Cu2+concentration (175 lM),theMDAproduction decreases with increasing quercetin concentra-tion (Fig. 1A). It is worth noting ... Flavonoids and urate in copper -induced low-density lipoprotein oxidation (Eur. J. Biochem. 271) 1993up to about 2 h. Finally, as shown in Fig. 2(C), slightlypro-oxidant concentrations of quercetin ... carotenoids and urate consumptionThe consumption of carotenoids was measured as an indexof the overall consumption of the endogenous antioxidantsof LDL during their Cu2+ -induced oxidation. As shown...
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Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf
... on the interaction betweenprealbumin, retinol-binding protein, and vitamin A.J Biol Chem 246, 44–49.71 Bellovino D, Morimoto T, Tosetti F & Gaetani S(1996) Retinol binding protein and transthyretin ... Thyroxine -induced con-formational changes in prealbumin. Biochemistry 17,5729–5733.69 Kanai M, Raz A & Goodman DS (1968) Retinol-bind-ing protein: the transport protein for vitamin A in human ... allostericeffect resulting from the occupancy of the first bindingsite [68]. TTR is also involved in the transportation ofretinol by forming a complex with the smaller retinol-binding protein (RBP) [69,70]....
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Báo cáo khoa học: "Sentence and Expression Level Annotation of Opinions in User-Generated Discourse" potx
... Scot-land.Minqing Hu and Bing Liu. 2004. Mining and sum-marizing customer reviews. In KDD’04: Proceed-ings of the Tenth ACM SIGKDD International Con-ference on Knowledge Discovery and Data Mining,pages ... match, and (iii) subset matching in which a span has tobe contained in another span in order to be consid-ered as a match.8Agreement naturally increases as we relax the matching constraints. ... subtasks including: creating opinion wordlexicons (Esuli and Sebastiani, 2006; Ding et al.,2008), identifying opinion expressions (Riloff and Wiebe, 2003; Fahrni and Klenner, 2008), identi-fying...
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Báo cáo khoa học: Determining and understanding the control of glycolysis in fast-growth tumor cells Flux control by an over-expressed but strongly product-inhibited hexokinase pdf
... N-terminal segment [54]. Hexokinase-I ispredominantly located in brain, kidney, retina and breast, whereas hexokinase-II is abundant in skeletalmuscle and adipose tissue [8]. In tumor cells, ... citrate and l- lactate were determined by standard enzymatic assays [61].Protein was determined by the biuret method using BSA as standard [62].Determination of intracellular glucose AS- 30D cells ... decrease the forwardreaction. In this regard, it is well documented thatGlc6P is a potent inhibitor of hexokinase-I, hexo-kinase-II and hexokinase-III [37]. In consequence, theincreased hexokinase...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... catalyzed by GDP-L-fucosepyrophosphorylase. We report here the cloning and expression of murineL-fucokinase and GDP-L-fucosepyrophosphorylase. MurineL-fucokinase is expressed as two transcripts ... fucokinase in mammalian cells The two splice variants of the murine fucokinase genes wereexpressed in COS-7 cells in frame with a 10-amino acid E2-Tag present in the pQM vector. The molecular massesof ... glycans,have an important role in inflammation. They initiateextravasation of leukocytes by mediating their tethering and rolling on the endothelium by decorating the leukocyte and endothelial cell counter...
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Báo cáo khoa học: Calcium and polyamine regulated calcium-sensing receptors in cardiac tissues doc
... an increase in [Ca2+]i induced by extracellular calcium was again observed(Fig.7A).Furthermore,increasing[Ca2+]ofrom 0–1.5 mMstill significantly increased intracellular calcium in 30 cells from ... occurring polyamines, including spermine,spermidine and putrescine, are involved in the synthesis ofnucleic acids and proteins in eukaryotic and prokaryotic cells. They play an important role in ... leading tointracellular calcium increase.Involvement of extracellular calcium entry in theextracellular calcium -induced increase in [Ca2+]iTo examine whether the increased [Ca2+]iwas...
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Báo cáo khoa học: Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar doc
... iron was removed by washing the cells withDMEM containing 50 lMDTPA and two more washingswith DMEM alone. IVI induced cell injury was assessed by measuring leakage of lactate dehydrogenase ... IVIwere added and changes in calcein -fluorescence weremeasured. Within the first 15 min of incubation with IVI,the LIP increased (i.e. baseline fluorescence decreased)between 8 and 25% depending on ... added. Control cells were incubated with cellculture medium alone. Iron taken up into the LIP was assessed by measuring the decrease in calcein fluorescence within 15 min at 37 °C. Shown are the...
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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... FEBSprotein in the two cell lines was determined as described in Materials and methods, and 81 lg of pro-tein was added to each well. The amount of S100A4 in the II-11b cells was 1.2% of that in the ... and TIMP-1.Previously, it has been shown that the reduced levelof S100A4 in the II-11b cells compared with pHb-1 cells resulted in a large reduction of in vivo and in vitroinvasive capacity, as well as in vitro ... MMPs are secreted as inactive proenzymes and latency is maintained by an interaction between acystein residue in the prodomain and Zn2+ in theactive site of the catalytic domain. Two major types...
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