Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf

... this protease at pH9 [10]. Further interest in the aromatic stacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple ... position. In the struc- tural deviation, the solvent ASA of the side-chain of His210 increased with the decrease in size of the side-chain at r...

Ngày tải lên: 21/02/2014, 03:20

... be involved in governing the activation mechanism of fumarate in the human and A. suum m-NAD-ME [27,32]. In our previous work, we delineated the functional role of Asp102, which is close to the ... Fumarate-binding site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate- binding site of m-NAD-ME. The corresponding ami...

Ngày tải lên: 23/03/2014, 06:20

... This kinetic analysis provided new insight into the role of the linker region. In addition, we fur- ther analyzed the functional role of the linker region based on the crystal structures of a ... exclude the effects of interacting proteins but can also be used to perform kinetic analy- ses of the native and linker-cleaved P-gp. In the present study, we inve...

Ngày tải lên: 29/03/2014, 23:20

... situated in the cC-domain of the fibrinogen ⁄ fibrin molecule [7]. On protofibril formation, polymerization site ‘B’ (Bb15– 18) is exposed at the N-terminal region of the fibrin b-chain, when fibrinopeptide ... BbArg14 is involved in another fibrin poly- merization site localized at the N-terminal fragment of the fibrin Bb-chain. This site is operationa...

Ngày tải lên: 30/03/2014, 03:20

... in these proteins. The results from the soluble mutants support the hypothesis that motif I interacts with galactose and that motif V plays a role in maintaining the structural integrity of the ... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis. That five of the 10 disease-causing mutations r...

Ngày tải lên: 08/03/2014, 02:20

... inhibi- tors in the solutions used during purification. Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table ... be the case with most of the mutations in GALE. Although all the mutant proteins were active, some of their kinetic properties differed from that of t...

Ngày tải lên: 23/03/2014, 11:20

... determine the affinity of the latent state (E ¼ in the above scheme) of the ATPase for IF 1 :K d is %1 l M .This value is much higher than the K d for the IF 1 –ATPase interaction in the absence of ... states of ATPase (or GTPase): where E is the only active form of ATPase. During ATP hydrolysis, IF1 can bind to the ATPase both in the absence and in the...

Ngày tải lên: 23/03/2014, 12:20

... Uridine phosphorylase () ⁄ )) murine embryonic stem cells clarify the key role of this enzyme in the regu- lation of the pyrimidine salvage pathway and in the acti- vation of fluoropyrimidines. ... enzymes, including uridine kinase, UMPK, and UMPS, in C. elegans to test their roles in 5-FU function and were able to identify homologs for most of them in C. elegans. T...

Ngày tải lên: 30/03/2014, 01:20

... strains revealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine from the start codon was substituted by thymine and none of the other sites ... acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the inve...

Ngày tải lên: 18/02/2014, 04:20

... chemokines stimu- lated mainly by in ammatory cytokines, including IL-1b, TNF-a and IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into the joints. In addition to functioning in ... polyarticular joint dis- ease that primarily affects the small joints of the hands and feet. The in ammatory process is characterized by in ltration of in ammatory cells...

Ngày tải lên: 18/02/2014, 18:20